Ca lectin gene insertion has the structural features of a transposable element

Vodkin, Lila O.; Rhodes, Patsy R.; Goldberg, Robert B.

doi:10.1016/0092-8674(83)90560-3

Cited by 265 publications

(147 citation statements)

References 30 publications

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“…These transposon tagging experiments indicate that mutated plant genes are preferably isolated from lines which 1) carry mutations that confer a clearly distinguishable phenotype and 2) have a low copy number of the active transposable element in their genome. However, until now, active transposons have been cloned only from Antirrhinum majus and Zea mays (for a review see Nevers et al [25]), although transposon-like elements have been isolated from several other plant species [7,14,30,34]. To extend the possibilities of transposon tagging to other plant species experiments have been performed to test the activity of the maize transposable element Ac in heterologous plant species.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

et al. 1989

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Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Ac excises from the HPTII gene with comparable frequencies (30~o) in both plant species. When the /~-glucuronidase (GUS) gene was used to detect transposition of Tam3, a significantly lower excision frequency (13~o) was found in both plant species. It could be shown that deletion of parts of the transposable elements Tam3 and Ac, removing either one of the terminal inverted repeats (TIR) or part of the presumptive transposase coding region, abolished the excision from the marker genes. This demonstrates that excision of the transposable element Tam3 in heterologous plant species, as documented for the autonomous element Ac, also depends on both properties. Southern blot hybridization shows the expected excision pattern and the reintegration of Tam3 and Ac elements into the genome of tobacco plants.

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Section: Introductionmentioning

confidence: 99%

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

et al. 1989

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“…The first transposable element discovered in soybean was Tgm (Vodkin et al, 1983), and consists of 7 different classes ranging in size from 1.6 kb to more than 12 kb (Rhodes and Vodkin, 1988). The copy number of this element is difficult to determine because of the variation in structure and organization of each class type.…”

Section: Current Status Of Soybean Genomicsmentioning

confidence: 99%

National Science Foundation-Sponsored Workshop Report. Draft Plan for Soybean Genomics

et al. 2004

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Recent efforts to coordinate and define a research strategy for soybean (Glycine max) genomics began with the establishment of a Soybean Genetics Executive Committee, which will serve as a communication focal point between the soybean research community and granting agencies. Secondly, a workshop was held to define a strategy to incorporate existing tools into a framework for advancing soybean genomics research. This workshop identified and ranked research priorities essential to making more informed decisions as to how to proceed with large scale sequencing and other genomics efforts. Most critical among these was the need to finalize a physical map and to obtain a better understanding of genome microstructure. Addressing these research needs will require pilot work on new technologies to demonstrate an ability to discriminate between recently duplicated regions in the soybean genome and pilot projects to analyze an adequate amount of random genome sequence to identify and catalog common repeats. The development of additional markers, reverse genetics tools, and bioinformatics is also necessary. Successful implementation of these goals will require close coordination among various working groups.

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“…En and Spm are functionally and structurally homologous maize transposons first identified in the early 1950s (Peterson, 1953;McClintock, 1954). They are archetypes of a family of related higher plant transposons: Mpil in maize (Weydemann et al, 1987), Tgml in soybean (Vodkin et al, 1983), Pisl in pea (Shirsat, 1988), and severa1 of the Tam elements in snapdragon (Bonas et al, 1984a(Bonas et al, , 1984bUpadhyaya et al, 1985); all members of the family cause 3-bp target site duplications and have TlRs nearly identical to the TlRs of EnlSpm. The 13-bp TlRs of EnlSpm have the sequence 5'-CACTACAAGAAAA-3' (Pereira et al, 1985), which matches four of the first five bases in the Jordan TIR, but only two of seven bases thereafter.…”

Section: Jordan 1s a Classical Eukaryotic Transposon Similar To Some mentioning

confidence: 99%

Jordan, an active Volvox transposable element similar to higher plant transposons.

1993

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We have isolated a 1595-bp transposable element from the multicellular green alga Volvox carteri following its insertion into the nitrate reductase (nitA) locus. This element, which we have named Jordan, has short (12-bp) terminal inverted repeats and creates a 3-bp target site duplication, like some higher plant transposons of the classic type. Contained within the first 200 bp of one end of the element are 55-bp inverted repeats, one of which begins with the terminal inverted repeat. Revertants of the transposon insertion into the nitA locus were obtained at a rate of . u~O -~ per Volvox embryo per generation. In each revertant examined, all transposon sequences were completely excised, but footprints containing both sets of duplicated bases, in addition to three to nine extra bases, were left behind. Jordan contains no significant open reading frames and so appean to be nonautonomous. DNA gel blot analysis indicates that Jordan is a member of a large family of homologous elements in the Volvox genome. We have isolated and characterized several of these homologs and found that they contain termini very similar to those of Jordan. Efforts to utilize Jordan and its homologs as tools to tag and clone developmentally interesting genes of Volvox are discussed.

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Ca lectin gene insertion has the structural features of a transposable element

Cited by 265 publications

References 30 publications

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

National Science Foundation-Sponsored Workshop Report. Draft Plan for Soybean Genomics

Jordan, an active Volvox transposable element similar to higher plant transposons.

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