Ca<sup>2+</sup>/H<sup>+</sup>exchange by acidic organelles regulates cell migration in vivo

Melchionda, Manuela; Pittman, Jon K.; Mayor, Roberto; Patel, Sandip

doi:10.1084/jem.2134oia28

Cited by 14 publications

(21 citation statements)

References 18 publications

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“…Metal tolerance assays were performed essentially as described previously [33] on solid SD -Ura medium with or without 3 mM MnCl2 or 100 µM CdCl2 metal salts, or in liquid yeast-peptone-dextrose (YPD) medium with or without 100 µM Al2(SO4)3, 75 µM CdCl2, 1 mM CoCl2, 1 mM CuSO4, 5 mM MnCl2, or 5 mM ZnSO4 metal salts. Internal Cd and Mn content in yeast grown in liquid YPD containing 30 µM CdCl2 or 2 mM MnCl2, respectively, was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES) as described previously [34].…”

Section: Yeast Heterologous Expression and Metal Tolerance Analysismentioning

confidence: 99%

Metal bioremediation by CrMTP4 over-expressing Chlamydomonas reinhardtii in comparison to natural wastewater-tolerant microalgae strains

et al. 2017

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Metal pollution in freshwater bodies is a long-standing challenge with large expense required to clean-up pollutants such as Cd. There is widespread interest in the potentially low-cost and sustainable use of biological material to perform bioremediation, such as the use of microalgae. Efficient metal bioremediation capacity requires both the ability to tolerate metal stress and metal accumulation. Here, the role of a Chlamydomonas reinhardtii metal tolerance protein (MTP) was examined for enhanced Cd tolerance and uptake. The CrMTP4 gene is a member of the Mn-CDF clade of the cation diffusion facilitator family of metal transporters but is able to provide tolerance and sequestration for Mn and Cd, but not other metals, when expressed in yeast. Over-expression of CrMTP4 in C. reinhardtii yielded a significant increase in tolerance to Cd toxicity and increased Cd accumulation although tolerance to Mn was not increased. In comparison, the metal tolerance of three chlorophyte microalgae strains (Chlorella luteoviridis, Parachlorella hussii, and Parachlorella kessleri) that had previously been adapted to wastewater growth was examined. In comparison to wild type C. reinhardtii, all three natural strains showed significantly increased tolerance to Cd, Cu, Al and Zn, and furthermore their Cd tolerance and uptake was greater than that of the CrMTP4 over-expression strains. Despite CrMTP4 gene over-expression being a successful strategy to enhance the Cd bioremediation potential of a metal-sensitive microalga, a single gene manipulation cannot compete with naturally adapted strain mechanisms that are likely to be multigenic and due in part to oxidative stress tolerance.

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Section: Yeast Heterologous Expression and Metal Tolerance Analysismentioning

confidence: 99%

Metal bioremediation by CrMTP4 over-expressing Chlamydomonas reinhardtii in comparison to natural wastewater-tolerant microalgae strains

et al. 2017

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“…95 Recent work has demonstrated that lysosomes are also responsible for Ca 2C release. 96,97 Ca 2C influx through plasma membrane channels is crucial for repulsion. 98 Therefore, we hypothesize that Reelin binds to ApoER2 and VLDLR on migrating neurons, triggers downstream signaling activation including SFKs, PI3K, FAK, PKC, ATP, NHE1 and AE1, eventually resulting in endocytosis of Reelin and its receptor complex.…”

Section: E1242455-6mentioning

confidence: 99%

How does Reelin signaling regulate the neuronal cytoskeleton during migration?

Chai¹,

Frotscher²

2016

Neurogenesis

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Neuronal migration is an essential step in the formation of laminated brain structures. In the developing cerebral cortex, pyramidal neurons migrate toward the Reelin-containing marginal zone. Reelin is an extracellular matrix protein synthesized by Cajal-Retzius cells. In this review, we summarize our recent results and hypotheses on how Reelin might regulate neuronal migration by acting on the actin and microtubule cytoskeleton. By binding to ApoER2 receptors on the migrating neurons, Reelin induces stabilization of the leading processes extending toward the marginal zone, which involves Dab1 phosphorylation, adhesion molecule expression, cofilin phosphorylation and inhibition of tau phosphorylation. By binding to VLDLR and integrin receptors, Reelin interacts with Lis1 and induces nuclear translocation, accompanied by the ubiquitination of phosphorylated Dab1. Eventually Reelin induces clustering of its receptors resulting in the endocytosis of a Reelin/receptor complex (particularly VLDLR). The resulting decrease in Reelin contributes to neuronal arrest at the marginal zone.

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“…All these major steps in cell migration are orchestrated by numerous scaffold, adaptor and adhesion proteins (e.g., actin, myosin, integrin, paxillin and tensin) in concerted actions that are regulated by various signalling molecules, including protein kinase C (PKC), mitogen-activated protein kinases [MAPK; c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38], Rho GTPase, Rho kinase, and focal adhesion kinase [ 1 , 7 – 9 ]. As the ubiquitous second messenger, cytosolic Ca 2+ plays an important role in regulating many cell functions, including cell migration, in response to diverse physical, chemical and biological clues from the surrounding environments [ 10 – 20 ].…”

Section: Introductionmentioning

confidence: 99%

ATP-induced Ca2+-signalling mechanisms in the regulation of mesenchymal stem cell migration

Jiang

Mousawi

Yang

et al. 2017

Cell. Mol. Life Sci.

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The ability of cells to migrate to the destined tissues or lesions is crucial for physiological processes from tissue morphogenesis, homeostasis and immune responses, and also for stem cell-based regenerative medicines. Cytosolic Ca2+ is a primary second messenger in the control and regulation of a wide range of cell functions including cell migration. Extracellular ATP, together with the cognate receptors on the cell surface, ligand-gated ion channel P2X receptors and a subset of G-protein-coupled P2Y receptors, represents common autocrine and/or paracrine Ca2+ signalling mechanisms. The P2X receptor ion channels mediate extracellular Ca2+ influx, whereas stimulation of the P2Y receptors triggers intracellular Ca2+ release from the endoplasmic reticulum (ER), and activation of both type of receptors thus can elevate the cytosolic Ca2+ concentration ([Ca2+]c), albeit with different kinetics and capacity. Reduction in the ER Ca2+ level following the P2Y receptor activation can further induce store-operated Ca2+ entry as a distinct Ca2+ influx pathway that contributes in ATP-induced increase in the [Ca2+]c. Mesenchymal stem cells (MSC) are a group of multipotent stem cells that grow from adult tissues and hold promising applications in tissue engineering and cell-based therapies treating a great and diverse number of diseases. There is increasing evidence to show constitutive or evoked ATP release from stem cells themselves or mature cells in the close vicinity. In this review, we discuss the mechanisms for ATP release and clearance, the receptors and ion channels participating in ATP-induced Ca2+ signalling and the roles of such signalling mechanisms in mediating ATP-induced regulation of MSC migration.

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Ca²⁺/H⁺exchange by acidic organelles regulates cell migration in vivo

Cited by 14 publications

References 18 publications

Metal bioremediation by CrMTP4 over-expressing Chlamydomonas reinhardtii in comparison to natural wastewater-tolerant microalgae strains

Metal bioremediation by CrMTP4 over-expressing Chlamydomonas reinhardtii in comparison to natural wastewater-tolerant microalgae strains

How does Reelin signaling regulate the neuronal cytoskeleton during migration?

ATP-induced Ca2+-signalling mechanisms in the regulation of mesenchymal stem cell migration

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Ca2+/H+exchange by acidic organelles regulates cell migration in vivo

Cited by 14 publications

References 18 publications

Metal bioremediation by CrMTP4 over-expressing Chlamydomonas reinhardtii in comparison to natural wastewater-tolerant microalgae strains

Metal bioremediation by CrMTP4 over-expressing Chlamydomonas reinhardtii in comparison to natural wastewater-tolerant microalgae strains

How does Reelin signaling regulate the neuronal cytoskeleton during migration?

ATP-induced Ca2+-signalling mechanisms in the regulation of mesenchymal stem cell migration

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Ca²⁺/H⁺exchange by acidic organelles regulates cell migration in vivo