Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa

Ulloa, Rita M.; Torres, Héctor N.; Ochatt, Claudia M.; Téllez-Iñón, Marı́a T.

doi:10.1007/bf00234573

Cited by 18 publications

(7 citation statements)

References 16 publications

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“…After incubating at room temperature for 10 min, 10 mL of the mixture were spotted onto P81 phosphocellulose squares (Whatman, Springfield Mill, Maidstone, UK) and the reaction was stopped by immersion in 150 mM H 3 PO 4 . After washing extensively with 150 mM H 3 PO 4 , the paper squares were dried and counted as described (Ulloa et al, 1991).…”

Section: In Vitro Protein Kinase Activity Assaymentioning

confidence: 99%

Nitric Oxide Mediates the Indole Acetic Acid Induction Activation of a Mitogen-Activated Protein Kinase Cascade Involved in Adventitious Root Development

et al. 2004

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Recently, it was demonstrated that nitric oxide (NO) and cGMP are involved in the auxin response during the adventitious rooting process in cucumber (Cucumis sativus; Pagnussat et al., 2002Pagnussat et al., , 2003. However, not much is known about the complex molecular network operating during the cell proliferation and morphogenesis triggered by auxins and NO in that process. Anatomical studies showed that formation of adventitious root primordia was clearly detected in indole acetic acid (IAA)-and NO-treated cucumber explants, while neither cell proliferation nor differentiation into root primordia could be observed in control explants 3 d after primary root was removed. In order to go further with signal transduction mechanisms that operate during IAA-and NO-induced adventitious root formation, experiments were designed to test the involvement of a mitogenactivated protein kinase (MAPK) cascade in that process. Cucumber explants were treated with the NO-donor sodium nitroprusside (SNP) or with SNP plus the specific NO-scavenger cPTIO. Protein extracts from those explants were assayed for protein kinase (PK) activity by using myelin basic protein (MBP) as substrate in both in vitro and in-gel assays. The activation of a PK of approximately 48 kD could be detected 1 d after NO treatment with a maximal activation after 3 d of treatment. In control explants, a PK activity was detected only after 4 d of treatment. The MBP-kinase activity was also detected in extracts from IAA-treated explants, while no signal was observed in IAA 1 cPTIO treatments. The PK activity could be inhibited by the cell-permeable MAPK kinase inhibitor PD098059, suggesting that the NO-dependent MBP-kinase activity is a MAPK. Furthermore, when PD098059 was administered to explants treated with SNP or IAA, it produced a delay in root emergence and a dose-dependent reduction in root number. Altogether, our results suggest that a MAPK signaling cascade is activated during the adventitious rooting process induced by IAA in a NO-mediated but cGMP-independent pathway. The activation of MAPKs is discussed in relation to the cell responses modulating mitotic process.Auxins play a central role in numerous developmental processes functioning as a signal for cell division, elongation and differentiation. Among auxin actions, an indole acetic acid (IAA) dependence of root formation, apical dominance, and tropic responses have been described (Theologis, 1986;Estelle, 1992;Davies, 1995). The molecular basis of auxin action is an area of intense study. Genetic approaches to dissect how this hormone triggers a variety of effects in plants rely on the analysis of mutants modified in their response to auxin (Walden and Lubenow, 1996). The available mutants display an array of phenotypes that indicates changes in auxin action or response including altered growth, changes in root length and apical dominance, and reduced gravitropism (Lincoln et al., 1990;Pickett et al., 1990;Wilson et al., 1990;Hobbie and Estelle, 1995;Leyser et al., 1996).Adventitious root for...

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Section: In Vitro Protein Kinase Activity Assaymentioning

confidence: 99%

Nitric Oxide Mediates the Indole Acetic Acid Induction Activation of a Mitogen-Activated Protein Kinase Cascade Involved in Adventitious Root Development

et al. 2004

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“…19 and 20). CaM kinases with properties similar to the mammalian CaM kinase II have been found in a number of species (21)(22)(23) including the sea urchin (24). Inhibition of the sea urchin CaM kinase II using either anti-CaM kinase antibodies or an inhibitory peptide was found to block nuclear envelope breakdown (24).…”

Section: -3-fold Treatment Of a Synchronous Population Of Hela Cellmentioning

confidence: 99%

Calcium/Calmodulin-dependent Phosphorylation and Activation of Human Cdc25-C at the G2/M Phase Transition in HeLa Cells

Patel

Holt

Philipova³

et al. 1999

Journal of Biological Chemistry

127

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“…Reactions were initiated by the addition of [ y-32P]ATP and stopped as described previously (Ulloa et al, 1991). Assays were performed at 3OoC for 10 min.…”

Section: Protein Kinase Activity Assaysmentioning

confidence: 99%

Changes in Calcium-Dependent Protein Kinase Activity during in Vitro Tuberization in Potato

et al. 1996

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Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa

Cited by 18 publications

References 16 publications

Nitric Oxide Mediates the Indole Acetic Acid Induction Activation of a Mitogen-Activated Protein Kinase Cascade Involved in Adventitious Root Development

Nitric Oxide Mediates the Indole Acetic Acid Induction Activation of a Mitogen-Activated Protein Kinase Cascade Involved in Adventitious Root Development

Calcium/Calmodulin-dependent Phosphorylation and Activation of Human Cdc25-C at the G2/M Phase Transition in HeLa Cells

Changes in Calcium-Dependent Protein Kinase Activity during in Vitro Tuberization in Potato

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