Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters

Abstract

Ca2+-regulated photoproteins of luminous marine coelenterates are of interest and a challenge for researchers as a unique bioluminescent system and as a promising analytical instrument for both in vivo and in vitro applications. The proteins are comprehensively studied as to biochemical properties, tertiary structures, bioluminescence mechanism, etc. This knowledge, along with available recombinant proteins serves the basis for development of unique bioluminescent detection systems that are “self-contained”, triggerable, fast, highly sensitive, and non-hazardous. In the paper, we focus on the use of photoproteins as reporters in binding assays based on immunological recognition element—bioluminescent immunoassay and hybridization immunoassay, their advantages and prospects.

Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins

“…We examined binding affinities of individual aptamers by the solid-phase bioluminescent sandwich microassay. A bioluminescent photoprotein obelin was chosen as a fast, triggerable, and highly sensitive reporter for the assay [20]. 3'-Biotinylated aptamers were immobilized on the streptavidinactivated surface (thus, employed as capture components of the sandwich) and titrated with total or glycated Hb.…”

Ultraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study

“…This is a basis for medico-biological application of the photoprotein bioluminescence in different types of diagnostics: for monitoring the Ca 2 + content, protein location in cells and tissues, and others. Additionally, bioluminescence of photoproteins is used as an intracellular marker [15,16]. These are reasons for intensive investigations of the photoprotein bioluminescence mechanism.…”

Improving the luminescence properties of aequorin by conjugating to CdSe/ZnS quantum dot nanoparticles: Red shift and slowing decay rate

“…Aequorin as a Ca 2+ -activation type is used for its unique advantages, such as detection within few seconds at the attomole level, high sensitivity and low background noise, high quantum yield, harmless application, lack of cellular toxicity, being active at physiological pH, hazard-free handling and noninvasive nature [16][17][18].…”