Improving the luminescence properties of aequorin by conjugating to CdSe/ZnS quantum dot nanoparticles: Red shift and slowing decay rate

Jalilian, Nezam; Shanehsaz, Maryam; Sajedi, Reza H.; Gharaat, Morteza; Ghahremanzadeh, Ramin

doi:10.1016/j.jphotobiol.2016.06.038

Cited by 6 publications

(3 citation statements)

References 68 publications

(105 reference statements)

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“…Another approach to obtain red-emitting photoproteins is to fuse them with fluorescent proteins or chemically attach them to fluorophores which act as BRET acceptors. For instance, aequorin has been conjugated to quantum-dot particles (CdSe/ZnS), resulting in the limited energy transfer with the presence of an emission peak around 540 nm [ 38 ]. The largest emission shift has been produced by fusing photoproteins with different variants of GFP (yellow, red, orange, etc.)…”

Section: Analytical Application Of Ca 2+ -Regulmentioning

confidence: 99%

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Krasitskaya

Bashmakova

Frank

2020

IJMS

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: The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.

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Section: Analytical Application Of Ca 2+ -Regulmentioning

confidence: 99%

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Krasitskaya

Bashmakova

Frank

2020

IJMS

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“…Some reports hint that the change in such emission decay is related to the structural rigidity of the photoprotein . In fact, the conjugation of quantum dots with aequorin increased its conformational rigidity, resulting in longer emission decay . On the one hand, mutation studies in aequorin showed that there is a relationship between sensitivity to Ca 2+ and reaction kinetics .…”

Section: Engineering Fp–photoprotein Sensors For Calcium Based On Bretmentioning

confidence: 99%

“…More recently, aequorin has been conjugated to quantum‐dot particles (CdSe/ZnS), resulting in limited energy transfer with the presence of an emission peak around 540 nm. In addition, a four‐fold increase in the decay time was observed, which was attributed to increased conformational rigidity . Further studies are needed in order to increase the BRET efficiency from aequorin to these conjugated fluorophores, for example using aequorin mutants to find the most suitable orientation/distance configuration for label attachment.…”

Section: Engineering Fp–photoprotein Sensors For Calcium Based On Bretmentioning

confidence: 99%

Fluorescent Protein–photoprotein Fusions and Their Applications in Calcium Imaging

Bakayan

Domingo

Vaquero

et al. 2017

Photochem & Photobiology

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Calcium‐activated photoproteins, such as aequorin, have been used as luminescent Ca2+ indicators since 1967. After the cloning of aequorin in 1985, microinjection was substituted by its heterologous expression, which opened the way for a widespread use. Molecular fusion of green fluorescent protein (GFP) to aequorin recapitulated the nonradiative energy transfer process that occurs in the jellyfish Aequorea victoria, from which these two proteins were obtained, resulting in an increase of light emission and a shift to longer wavelength. The abundance and location of the chimera are seen by fluorescence, whereas its luminescence reports Ca2+ levels. GFP‐aequorin is broadly used in an increasing number of studies, from organelles and cells to intact organisms. By fusing other fluorescent proteins to aequorin, the available luminescence color palette has been expanded for multiplexing assays and for in vivo measurements. In this report, we will attempt to review the various photoproteins available, their reported fusions with fluorescent proteins and their biological applications to image Ca2+ dynamics in organelles, cells, tissue explants and in live organisms.

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CdTe quantum dots with green fluorescence generated by bioluminescence resonance energy transfer from aequorin

Jalilian

Sajedi

Shanehsaz³

et al. 2016

Microchim Acta

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Improving the luminescence properties of aequorin by conjugating to CdSe/ZnS quantum dot nanoparticles: Red shift and slowing decay rate

Cited by 6 publications

References 68 publications

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Fluorescent Protein–photoprotein Fusions and Their Applications in Calcium Imaging

CdTe quantum dots with green fluorescence generated by bioluminescence resonance energy transfer from aequorin

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