Mucilage fractions extracted from Opuntiaficus-indicacactus were captured in calcium alginate biobeads. The beads were tested as eco-friendly adsorbents to removearsenic (As) from water, and their Asremoval efficiency was evaluated. Batch adsorption studies were performed using an incomplete factorial design by varying the concentrations of mucilage (0.5-2 mgL-1), sodium alginate (3-5%), and calcium chloride (0.5-1 molL-1) incorporated in the biobeads. The optimal formulation of these adsorbentbiobeads varied depending on the mucilage fraction. Thus, biocompositeformulated with 1.25 mgL-1 of gelling mucilage, 4% of sodium alginate, and 0.75 molL-1 of calcium chloride eliminated up to 63% of As from water, with a capacity of 101.6 mgg-1 , whereas the composite biobeadsformulated with 1.25 mgL-1 of non-gelling mucilage reduced59.8% of As from water, using the least amounts of sodium alginate and calcium chloride in the range tested, with a capacity of 97.1 mgg-1 .