Cadherin and vimentin immunoexpression in the testis of normal and induced infertility models of albino rats

ElGhamrawy, T. A.; Helmy, Dina Omar; Elall, H. F.A.

doi:10.5603/fm.2014.0050

Cited by 16 publications

(17 citation statements)

References 26 publications

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“…That is in agreement with previous investigators who mentioned that, decreased vimentin filaments in Sertoli cell leads to detachment of spermatogenic cells from Sertoli cells with loss of the support and nutrition provided by Sertoli [34] . In addition, vimentin was reported to be one of cell junction proteins which is important for normal spermatogenesis and hence, the decreased density of vimentin could lead to blood-testis barrier disruption with spermatogenesis impairment [35] .…”

Section: Discussionmentioning

confidence: 99%

Role of bone marrow derived mesenchymal stem cells in protection of spermatogenic and Sertoli cells against histological alterations induced by torsion/detorsion in rats

Reda

Hashem

Elnegris

et al. 2017

Journal of Medical Histology

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Background and Objectives: Testicular torsion is an emergency. Thus, early diagnosis and appropriate treatment are fundamental to avoid irreversible testicular damage. The present study aimed to throw more light on the histological alterations in adult albino rat's ipsilateral testis after unilateral testicular torsion and detorsion (T/D) and to demonstrate the effect of mesenchymal stem cells (MSCs) injection on these alterations. Materials and Methods: Twenty seven adult healthy male albino rats were divided into 3 main equal groups: Group I (Control group), Group II (T/D): the rats were surgically operated to perform T/D and Group III (T/D+MSCs): the animals were injected with MSCs in tail vein at the end of the surgery. All rats were sacrificed ten weeks from the start of the experiment and testis specimens were processed to be examined by light and electron microscope. Blood specimens were taken to measure serum testosterone level. Results: Degenerative changes were observed in spermatogenic and Sertoli cells of T/D group and were associated with statistical significant reduction in serum testosterone level, height of germinal epithelium, proliferating cell nuclear antigen (PCNA) and vimentin immunoexpression. These changes were observed to be reduced in T/D+MSCs group. Conclusions: MSCs treatment could protect the spermatogenic and Sertoli cells from degenerative changes in testis after T/D in adult albino rats. Hence, MSCs therapy could be considered as a promising therapeutic tool to preserve spermatogenesis in cases of testicular T/D and should be more studied either on experimental animals or human.

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Section: Discussionmentioning

confidence: 99%

Role of bone marrow derived mesenchymal stem cells in protection of spermatogenic and Sertoli cells against histological alterations induced by torsion/detorsion in rats

Reda

Hashem

Elnegris

et al. 2017

Journal of Medical Histology

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“…PBS 0.01 mmol/l was applied instead of primary antibodies as a negative control. Positive control : human rhabdosarcoma cell line JR1 [24] .…”

Section: Immunohistochemical Studymentioning

confidence: 99%

The Possible Role of Curcumin against Changes Caused by Paracetamol in Testis of Adult Albino Rat ( Histological, Immunohistochemical and Biochemical Study)

Mohammed

Sabry

2019

Egyptian Journal of Histology

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Background: Paracetamol is widely used analgesic which was wrongly thought to be entirely harmless by lots of people. Curcumin has many pharmacological uses such as antibacterial, antiviral, antifungal and anticancer. The aim of the Work: This study aimed to evaluate the histopathological effects of paracetamol on testicular tissue and how far curcumin can protect testicular tissue from these changes. Material and Methods: Twenty eight adult rats were used in this work. Rats were equally divided into four groups. Group I: the control group was divided into(subgroup a,b given distilled water ,corn oil respectively ),Group II: was given curcumin 50mg\kg body weight, Group III: was given single daily dose of paracetamol 500mg\ kg for five days ,Group IV: pretreated with curcumin 50mg\kg\body weight for 10 days then received single daily dose of paracetamol 500mg\kg in association with curcumin for five days. In the present work, the sperm count, histological and immunohistochemical,biochemical and morphometric studies were evaluated. Results: Paracetamol caused loss of normal architecture of testicular tissue, wide interstitial spaces , loss of stratal arrangement of germinal epithelium with intercellular spacing. Also,reduction in number of +ve vimentin staining Sertoli cells and marked decrease in number and intensity of PCNA positive cells with increased oxidative stress in testicular tissue. Curcumin protected testis against these alterations. Conclusion: Curcumin, is effective in reducing paracetamol testicular toxicity in albino rat, by ameliorating oxidative stress, histopathological and immunhistochemical changes and restoring the normal testicular tissue architecture and function and this can be considered for humans.

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“…Upon continuous treatment with busulfan in adult rats, the apical VFs in the SCs gradually collapse towards the nuclei, becoming disorganized in the basal region of the SCs. After the effect of busulfan effect subsides, spermatogenesis begins to be restored and the VFs reorganize in the basal and perinuclear regions of the SCs among the spermatogonia and spermatocytes (ElGhamrawy et al 2014;Kopecky et al 2005). ICAM-1 is expressed by several cell types (such as: SCs and germ cells), and it regulates the movement of preleptotene/leptotene spermatocytes across the BTB and the release of elongated spermatids into the tubule lumen (Mruk and Cheng 2004;Xiao et al 2012).…”

Section: Busulfan Destroys Vimentin Filaments (Vfs) and Intercellular Cell Adhesion Molecule-1 (Icam-1)mentioning

confidence: 99%

Progress on the study of the mechanism of busulfan cytotoxicity

2018

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The preparation of spermatogonial stem cell (SSC) transplant recipients laid the technical foundation for SSC transplant technology and the understanding of spermatogenesis mechanisms. Busulfan is commonly used to prepare recipients for mouse SSC transplantation; however, its safety and efficiency have been questioned. This review summarizes the relationship between SSCs and Sertoli cells (SCs), and the mechanism of busulfan toxicity against sperm cells. We concluded that the proliferation, differentiation, and apoptosis of SSCs are regulated by SCs. The endogenous spermatogenic cells are depleted by busulfan treatment via alkylation of DNA, destruction of vimentin filament distribution, disruption of SSC differentiation, promotion of SSC dormancy, and generation of oxidative stress. However, the mechanisms require further exploration. The recent establishment of a model in vitro culture system has provided a good technical foundation to further explore these mechanisms, which will help us to find more efficient methods of recipient preparation and optimal transplantation times.

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Cadherin and vimentin immunoexpression in the testis of normal and induced infertility models of albino rats

Abstract: (Folia Morphol 2014; 73, 3: 339-346)

Cited by 16 publications

References 26 publications

Role of bone marrow derived mesenchymal stem cells in protection of spermatogenic and Sertoli cells against histological alterations induced by torsion/detorsion in rats

Role of bone marrow derived mesenchymal stem cells in protection of spermatogenic and Sertoli cells against histological alterations induced by torsion/detorsion in rats

The Possible Role of Curcumin against Changes Caused by Paracetamol in Testis of Adult Albino Rat ( Histological, Immunohistochemical and Biochemical Study)

Progress on the study of the mechanism of busulfan cytotoxicity

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