Role of bone marrow derived mesenchymal stem cells in protection of spermatogenic and Sertoli cells against histological alterations induced by torsion/detorsion in rats

Abstract

Background and Objectives: Testicular torsion is an emergency. Thus, early diagnosis and appropriate treatment are fundamental to avoid irreversible testicular damage. The present study aimed to throw more light on the histological alterations in adult albino rat's ipsilateral testis after unilateral testicular torsion and detorsion (T/D) and to demonstrate the effect of mesenchymal stem cells (MSCs) injection on these alterations. Materials and Methods: Twenty seven adult healthy male albino rats were divided into 3 main equal groups: Group I (Control group), Group II (T/D): the rats were surgically operated to perform T/D and Group III (T/D+MSCs): the animals were injected with MSCs in tail vein at the end of the surgery. All rats were sacrificed ten weeks from the start of the experiment and testis specimens were processed to be examined by light and electron microscope. Blood specimens were taken to measure serum testosterone level. Results: Degenerative changes were observed in spermatogenic and Sertoli cells of T/D group and were associated with statistical significant reduction in serum testosterone level, height of germinal epithelium, proliferating cell nuclear antigen (PCNA) and vimentin immunoexpression. These changes were observed to be reduced in T/D+MSCs group. Conclusions: MSCs treatment could protect the spermatogenic and Sertoli cells from degenerative changes in testis after T/D in adult albino rats. Hence, MSCs therapy could be considered as a promising therapeutic tool to preserve spermatogenesis in cases of testicular T/D and should be more studied either on experimental animals or human.

The effect of chronic stress on the testis of adult albino Rats and the possible protective effect of astaxanthin supplementation (Histological, immunohistochemical and biochemical studies)

“…In the present work, some sloughed spermatids with eccentric irregular shrunken pyknotic nuclei were noticed. Similar findings were observed by Reda et al [34] . Also, some sperms appeared deformed and degenerated with vacuolated mitochondria.…”

“…In the current study, statistical analysis of the results showed a significant decrease in area % of Vimentin of stress group when compared with control group. Similar results were previously described by Chen et al [35] and Reda et al [34] who suggested that oxidative stress may reduce vimentin filaments in the testis.…”

“…Vimentin is an intermediate filament protein expressed in Sertoli cells providing them structural and functional support. So, the study of vimentin expression could be useful to understand the changes in Sertoli cells [34] . In the current study, statistical analysis of the results showed a significant decrease in area % of Vimentin of stress group when compared with control group.…”

Impact of chitosan administration on titanium dioxide nanoparticles induced testicular dysfunction

“…PCNA immune-expression in the testis is used as a proliferative marker for spermatogenesis estimation 45 , 46 . It is considered a rapid, reliable, sensitive, and quantitative approach for determining and detecting early testicular toxicity 47 , 48 . Spermatogenesis is a dynamic and synchronized process of maturation of stem spermatogonia into mature spermatozoa, which takes place in the seminiferous tubules 49 .…”