Cadmium and zinc-mediated oxidative burst in tobacco BY-2 cell suspension cultures

Źróbek-Sokolnik, Anna; Asard, Han; Górska-Koplińska, Kamilla; Górecki, Ryszard J.

doi:10.1007/s11738-008-0197-8

Cited by 16 publications

(9 citation statements)

References 41 publications

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“…Seven days after exposure to both Zn concentrations, significantly higher carbonyl content was measured in comparison to control and other treatments. These results are in accordance with elevated MDA values in abovementioned treatments and could be explained by the findings that Zn in higher concentrations can also induce oxidative stress in various plant species (Drost et al 2007;Ź róbek-Sokolnik et al 2009). Moreover, exposure to combinations of Cd and Zn in our experiment resulted in lower carbonyl content in comparison to plants treated with Zn alone, probably reflecting the reduced concentration of Zn in plants.…”

Section: Discussionsupporting

confidence: 88%

Biochemical responses of Lemna minor experimentally exposed to cadmium and zinc

et al. 2011

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The effects of 5 μM cadmium (Cd), a non-essential toxic element and 25 and 50 μM zinc (Zn), an essential micronutrient, were investigated in aquatic plant Lemna minor L. after 4 and 7 days of exposure to each metal alone or to their combinations. Both metals showed tendency to accumulate with time, but when present in combination, they reduced uptake of each other. Cd treatment increased the lipid peroxidation and protein oxidation indicating appearance of oxidative stress. However, Zn supplementation in either concentration reduced values of both parameters, while exposure to Zn alone resulted in elevated level of lipid peroxidation and protein oxidation but only on the 7th day. Enhanced DNA damage, which was found on the 4th day in plants treated with Cd alone or in combination with Zn, was reduced on the 7th day in combined treatments. Higher catalase activity obtained in all treated plants on the 4th day of experiment was reduced in Zn-treated plants, but remained high in plants exposed to Cd alone or in combination with Zn after 7 days. Cd exposure resulted in higher peroxidase activity, while Zn addition prominently reduced peroxidase activity in the plants subjected to Cd stress. In conclusion, Cd induced more pronounced oxidative stress and DNA damage than Zn in applied concentrations. Combined treatments showed lower values of oxidative stress parameters--lipid peroxidation, protein oxidation and peroxidase activity as well as lower DNA damage, which indicates alleviating effect of Zn on oxidative stress in Cd-treated plants.

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Section: Discussionsupporting

confidence: 88%

Biochemical responses of Lemna minor experimentally exposed to cadmium and zinc

et al. 2011

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“…The presence of extracellular ROS has been demonstrated in green algae (Knauert & Knauer, 2008), plants (Langebartels et al, 2002;#róbek-Sokolnik et al, 2009), fungi (Jarosz-Wilkolazka et al, 1998) and lichens (Beckett et al, 2003) exposed to heavy metals and other stress conditions. Oidiodendron maius Zn also releases OmSod1 in the extracellular environment, where it could deal with heavy metals and exogenous sources of superoxide.…”

Section: Discussionmentioning

confidence: 99%

“…Upon exposure to different stress, ROS can form not only in the cell, from where they could diffuse through the plasma membrane, but also directly in the extracellular compartment. The presence of extracellular ROS has been demonstrated in green algae (Knauert & Knauer, 2008), plants (Langebartels et al, 2002;#róbek-Sokolnik et al, 2009), fungi (Jarosz-Wilkolazka et al, 1998) and lichens (Beckett et al, 2003) exposed to heavy metals and other stress conditions. Growth performance of transformed OmSOD1-carrying yeasts plated on medium containing different heavy metals vs. vector-carrying yeasts.…”

Section: Discussionmentioning

confidence: 99%

Cu,Zn superoxide dismutase and zinc stress in the metal-tolerant ericoid mycorrhizal fungusOidiodendron maiusZn

Vallino¹,

Martino²,

Boella³

et al. 2009

FEMS Microbiology Letters

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The sequence encoding a superoxide dismutase (SOD) was isolated from the cDNA library of a zinc-tolerant strain of the ericoid mycorrhizal fungus Oidiodendron maius, grown under zinc-stress conditions. Sequence homology to other SODs strongly suggests that it is a copper- and zinc-containing SOD. Functional complementation assays showed that the gene confers increased tolerance to zinc and copper stress to a Cu,ZnSOD-defective yeast mutant. Monitoring of transcript and protein levels following zinc stress suggests that OmSOD1 expression is controlled at the transcriptional level. The OmSod1 protein was found both in the cell extract and in the growth medium of viable fungal cultures. This is the first characterization of an extracellular Cu,ZnSOD in a mycorrhizal fungus. In nature, the presence of OmSod1 in the extracellular environment may also extend the protective role of this enzyme to the plant symbiont. This may be of particular interest from the perspective of using mycorrhizal fungi in bioremediation programmes.

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“…The induction of ROS production due to metals (cadmium and zinc) in Nicotiana tabacum L. cv. Bright Yellow 2 (TBY-2) cells in suspension cultures showed properties comparable to the elicitor-induced oxidative burst in other plant cells [261]. Redox-active metals, such as iron, copper, and chromium, undergo redox cycling producing ROS, whereas redox-inactive metals, such as lead, cadmium, mercury, and others, deplete cell's major antioxidants, particularly thiolcontaining antioxidants and enzymes [6,11,[14][15][16][262][263][264].…”

Section: Metal Toxicitymentioning

confidence: 99%

Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

et al. 2012

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Reactive oxygen species (ROS) are produced as a normal product of plant cellular metabolism. Various environmental stresses lead to excessive production of ROS causing progressive oxidative damage and ultimately cell death. Despite their destructive activity, they are well-described second messengers in a variety of cellular processes, including conferment of tolerance to various environmental stresses. Whether ROS would serve as signaling molecules or could cause oxidative damage to the tissues depends on the delicate equilibrium between ROS production, and their scavenging. Efficient scavenging of ROS produced during various environmental stresses requires the action of several nonenzymatic as well as enzymatic antioxidants present in the tissues. In this paper, we describe the generation, sites of production and role of ROS as messenger molecules as well as inducers of oxidative damage. Further, the antioxidative defense mechanisms operating in the cells for scavenging of ROS overproduced under various stressful conditions of the environment have been discussed in detail.

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Cadmium and zinc-mediated oxidative burst in tobacco BY-2 cell suspension cultures

Cited by 16 publications

References 41 publications

Biochemical responses of Lemna minor experimentally exposed to cadmium and zinc

Biochemical responses of Lemna minor experimentally exposed to cadmium and zinc

Cu,Zn superoxide dismutase and zinc stress in the metal-tolerant ericoid mycorrhizal fungusOidiodendron maiusZn

Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions

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