2004
DOI: 10.1242/jeb.01152
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Cadmium-induced apoptosis in oyster hemocytes involves disturbance of cellular energy balance but no mitochondrial permeability transition

Abstract: SUMMARY Exposure to environmentally prevalent heavy metals such as cadmium can have detrimental effects on a variety of commercially and ecologically important species such as oysters. Since Cd2+ is known to induce apoptosis in immune cells of vertebrates, we have investigated the effects of this metal on isolated oyster hemocytes, the main cellular immune defense in mollusks. Enhanced apoptosis of these cells could conceivably create immunosuppressed conditions in these organisms and result in … Show more

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Cited by 192 publications
(120 citation statements)
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“…The first two mechanisms are unlikely to play a significant role in our in vitro system that was devoid of free fatty acids (FFAs) due to the presence of fatty acid-free BSA used to scavenge FFAs during mitochondrial isolation and assays, and did not contain AMP, the most potent allosteric stimulator of ANT (53). Our earlier studies also indicate that Cd does not induce opening of the MPT pore in oyster mitochondria (67). We did not measure the rates of Ca 2ϩ cycling in Cd-exposed oyster mitochondria; however, earlier studies in mammalian and plant systems show that Cd 2ϩ and other bivalent metals such as Hg 2ϩ may interfere with Ca 2ϩ homeostasis stimulating Ca 2ϩ efflux from intracellular storage sites (27,45,63,74).…”
Section: R26mentioning
confidence: 90%
“…The first two mechanisms are unlikely to play a significant role in our in vitro system that was devoid of free fatty acids (FFAs) due to the presence of fatty acid-free BSA used to scavenge FFAs during mitochondrial isolation and assays, and did not contain AMP, the most potent allosteric stimulator of ANT (53). Our earlier studies also indicate that Cd does not induce opening of the MPT pore in oyster mitochondria (67). We did not measure the rates of Ca 2ϩ cycling in Cd-exposed oyster mitochondria; however, earlier studies in mammalian and plant systems show that Cd 2ϩ and other bivalent metals such as Hg 2ϩ may interfere with Ca 2ϩ homeostasis stimulating Ca 2ϩ efflux from intracellular storage sites (27,45,63,74).…”
Section: R26mentioning
confidence: 90%
“…Noteworthy, in marine invertebrates Cd-triggered apoptosis can take place in the absence of significant alterations of mitochondrial membrane permeability [52]. On the other hand, the prolonged production of ROS can cause the disruption of the mitochondrial electron transport and strongly interfere with mitochondrial permeability transition pores, as reported in other model systems [48,50].…”
Section: Mitochondria-related Activitymentioning
confidence: 93%
“…Next, 400 ml 1Â Annexin-V Binding Buffer was added to each tube. Finally, the hemocytes were incubated at room temperature for 15 min in the dark and then analyzed by flow cytometry for fluorescence in the FL-1 (Annexin V) and FL-2 (PI) channels [29].…”
Section: Apoptosis Detectionmentioning
confidence: 99%