Caffeine alters mitochondrial dehydrogenase and alkaline phosphatase activity of human gingival fibroblasts in vitro

Abstract

These results provided evidence that caffeine in high concentrations can decrease cellular viability and ALP activity in HGF.

Caffeine administration modulates TGF-β signaling but does not attenuate blunted alveolarization in a hyperoxia-based mouse model of bronchopulmonary dysplasia

“…A higher caffeine concentration (5 mmol/l) was tolerated by ATII cells, but not fibroblasts, and 25 mmol/l was not tolerated by either cell-type, where pronounced cell death was noted (data not shown). This caffeine range for cell viability is in line with the reports of others documenting safe doses of caffeine up to 1 mmol/l for fibroblasts and epithelial cells (9,23). Caffeine administration increased mRNA expression of the type I TGF-β receptor (TGFβR1, Tgfbr1; Figure 1a) and the TGFβ3 ligand (Tgfb3; Supplementary Figure S1 online) in mouse lung fibroblasts.…”

Autophagy and glycolysis independently attenuate silibinin-induced apoptosis in human hepatocarcinoma HepG2 and Hep3B cells

“…The reduction of 3-[4,5-dimethylthiazole-2yl]-2,5-diphenyltetrazolium bromide (MTT) to MTTformazan is catalyzed by mitochondrial succinate dehydrogenase. 28 HepG2 or Hep3B cells were seeded in 96-well cell culture plates (Corning, NY, USA) at a density of 4.0 � 10 3 cells/well and further cultured for 24 h. After the cells were subjected to the indicated treatments for 24 h, the cells were rinsed twice with ice-cold PBS. They were incubated with 100 mL of 0.5 mg/mL MTT solution at 37 � C for 3 h. Then the supernatant was discarded, and the formazan dye product of residual cell layer was solubilized with 150 mL of DMSO.…”