Caffeine effects on meiotic maturation in hamster oocytes in vitro

Prather, Angela L.; Racowsky, Catherine

doi:10.1016/0890-6238(92)90193-w

Cited by 10 publications

(6 citation statements)

References 33 publications

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“…The majority of caffeine-treated oocytes had a germinal vesicle with a nucleolus surrounded by ring-or horseshoe-shaped chromatin. Moreover, our data also support the results of a study using hamster oocytes, where caffeine at the concentrations of 0.17-2 mM induced meiotic arrest dosedependently (Prather & Racowsky, 1992). In these earlier studies, the presence of dbcAMP in the medium completely inhibited the meiotic resumption of cumulus-enclosed porcine oocytes for 20 h, and its inhibitory effect was reversible.…”

Section: Discussionsupporting

confidence: 91%

“…The authors found that doses of caffeine higher than 450 µg/ml in the culture medium inhibited oocyte maturation. Recently, similar inhibitory effects of caffeine were observed in hamster oocytes (Prather & Racowsky, 1992). These results suggest that caffeine, like other purine derivatives, probably inhibits cAMP phosphodiesterase, Cdc2 kinase activation (Racowsky, 1993;Chesnel et al, 1994) and meiotic maturation.…”

Section: Introductionsupporting

confidence: 71%

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Effect of caffeine on meiotic maturation of porcine oocytes

Kren¹,

Ogushi

Miyano

2004

Zygote

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This study was conducted to evaluate the effect of caffeine on the meiotic maturation of porcine oocytes. Oocyte-cumulus complexes were collected from slaughterhouse-derived ovaries and cultured for 24, 32 or 48 h in medium 199 supplemented with 10% fetal calf serum, 10 µg/ml FSH, 50 µg/ml sodium pyruvate and 50 µg/ml gentamicin in the presence or absence of 2.5 mM caffeine. Caffeine inhibited the meiotic resumption of pig oocytes effectively after 24 h of culture, and 95.5% of oocytes were arrested at the germinal vesicle (GV) stage (control 17.8%, p < 0.05). Prolonged culture with caffeine up to 32 h or 48 h, however, resulted in a significant decrease in the inhibitory effect (GV: 13.8% and 8.2%). The number of oocytes at metaphase II after 48 h of culture in the presence of caffeine was significantly lower than that in the control medium (65.3% vs 94.7%, p < 0.05). The withdrawal of caffeine after 24 h of culture resulted in the resumption of meiotic maturation, and the oocytes reached metaphase II after 48 h. However, the ability of caffeine-treated oocytes to develop to blastocysts after artificial activation was lower than that of the control (5.5% vs 9.1%, p < 0.05). Caffeine treatment significantly increased cAMP levels in the oocytes after 24 h of culture, while both Cdc2 kinase and MAP kinase activation were inhibited in the oocytes. These results suggest that caffeine, similarly to other purine derivatives, prolongs the meiotic arrest of porcine oocytes at the GV stage, perhaps by its action of increasing the cAMP level and by the suppression of Cdc2 kinase and MAP kinase activities in the oocytes.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionsupporting

confidence: 71%

Effect of caffeine on meiotic maturation of porcine oocytes

Kren¹,

Ogushi

Miyano

2004

Zygote

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“…These results suggest that fragmentation can be suppressed by treatment with caffeine, which is quite in agreement with our previous report after prolonged maturation (up to 72 h) by the maturation system, whereby the decrease in MPF activity was suppressed by caffeine supplementation (Kikuchi et al, 2000(Kikuchi et al, , 2002a. Although caffeine has been reported to have an inhibitory effect on oocyte maturation at the earlier stages (Jagiello et al, 1972;Prather & Racowsky, 1992;Racowsky, 1993;Chesnel et al, 1994;Kren et al, 2004), to our knowledge no study of the effect of caffeine on the parthenogenetic developmental ability of aged porcine oocytes has previously been reported.…”

Section: Activation and Development Of Aged Porcine Oocytessupporting

confidence: 93%

“…This has also been reported in cultured mammalian cells (Steinmann et al, 1991;Poon et al, 1997;Matsumoto et al, 2000), Xenopus laevis oocytes (Smythe & Newport, 1992) and porcine oocytes (Kikuchi et al, 2000(Kikuchi et al, , 2002a, but only in regard to the events of porcine oocyte activation and not in terms of further development to the early embryonic stages. The effects of caffeine on cAMP phosphodiesterase or MPF activation in in vitro cultured mammalian oocytes have been confirmed (Racowsky, 1993;Chesnel et al, 1994, Kren et al, 2004, and this chemical probably inhibits meiotic maturation (Jagiello et al, 1972;Prather & Racowsky, 1992). However, the effects of caffeine during oocyte aging on embryonic development have not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

Effects of caffeine treatment on aged porcine oocytes: parthenogenetic activation ability, chromosome condensation and development to the blastocyst stage after somatic cell nuclear transfer

Iwamoto

Ōnishi

Fuchimoto

et al. 2005

Zygote

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The possibility of using aged porcine oocytes treated with caffeine, which inhibits the decrease in M-phase promoting factor activity, for pig cloning was evaluated. Cumulus-oocyte complexes (COCs) were cultured initially for 36 h and subsequently with or without 5 mM caffeine for 24 h (in total for 60 h: 60CA+ or 60CA- group, respectively). As a control group, COCs were cultured for 48 h without caffeine (48CA-). The pronuclear formation rates at 10 h after electrical stimulation in the 60CA+ and 60CA- groups decreased significantly (p < 0.05) compared with the 48CA- group. However, the fragmentation rate was significantly higher (p < 0.05) in the 60CA- group than in the 60CA+ and 48CA- groups. When the stimulated oocytes were cultured for 6 days, the 60CA+ group showed significantly lower blastocyst formation and higher fragmentation or degeneration rates (p < 0.05) than the 48CA- group. However, the number of total cells in blastocysts was not affected by maturation period or caffeine treatment. When somatic cell nuclei were injected into the non-enucleated oocytes and exposed to cytoplasm for a certain duration (1-11 h) before the completion of maturation (48 or 60 h), the rate of nuclear membrane breakdown after exposure to cytoplasm for 1-2 h in the 60CA- oocytes was significantly lower (p < 0.05) than in the other experimental groups. The rate of scattered chromosome formation in the same 60CA- group tended to be lower (p = 0.08) than in the other groups. After the enucleation and transfer of nuclei, blastocyst formation rates in the 60CA+ and 60CA- groups were significantly lower (p < 0.05) than in the 48CA- group. Blastocyst quality did not differ among all the groups. These results suggest that chromosome decondensation of the transplanted somatic nucleus is affected by both the duration of exposure to cytoplasm and the age of the recipient porcine oocytes, and that caffeine treatment promotes nuclear remodelling but does not prevent the decrease in the developmental ability of cloned embryos caused by oocyte aging.

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“…Following a surge of luteinizing hormone, or separation from follicular cells in vitro, the level of cAMP in oocytes declines, meiosis resumes, and oocytes advance to metaphase II (MII) and await fertilization (Mehlmann, 2005). Maturation of oocytes removed from their follicles can be inhibited by membrane permeant analogs of cAMP (Sato et al, 1985;Warikoo and Bavister, 1989) or by inhibitors of cAMP-specific phosphodiesterases, such as hypoxanthine (Warikoo and Bavister, 1989;Fagbohun and Downs, 1990) or the methylxanthines caffeine (Jagiello et al, 1972;Prather and Racowsky, 1992;Kren et al, 2004) or 3isobutyl-1-methylxanthine (Fagbohun and Downs, 1990;Tsafriri and Reich, 1999). Due to the potential inhibitory effects of activator compounds on oocyte maturation, practitioners of monkey IVF select oocytes retrieved from superovulated females that have advanced to metaphase II (Wolf, 2004).…”

Section: Discussionmentioning

confidence: 99%

Release of DEFB126 from macaque sperm and completion of capacitation are triggered by conditions that simulate periovulatory oviductal fluid

Tollner¹,

VandeVoort²,

Yudin³

et al. 2008

Molecular Reproduction Devel

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Capacitation of macaque sperm in vitro has been achieved efficiently only with the addition of both cyclic nucleotides and methylxanthines. The use of these exogenous sperm activators clouds an understanding of the normal mechanisms underlying capacitation and may slow early embryo development following in vitro fertilization (IVF). We demonstrate that culture medium which simulates periovulatory oviductal fluid with respect to bicarbonate (HCO(3)(-)) and glucose concentration induces capacitation in a high percentage of macaque sperm as determined by the ability of sperm to undergo both the release of coating protein DEFB126 and the zona pellucida-induced acrosome reaction (AR). Few sperm were able to undergo the AR following 6 hr incubation in medium containing either 35 mM HCO(3)(-) (approximately 7.2 pH) or 90 mM HCO(3)(-) (approximately pH 7.8) with 5 mM glucose. When glucose concentration was lowered to 0.5 mM to match levels reported for women at midcycle, the AR rate increased significantly in sperm incubated in both levels of HCO(3)(-), indicating that glucose interferes with sperm responsiveness to increasing HCO(3)(-) concentration observed in the primate oviduct during ovulation. Even greater synchronization of capacitation could be achieved with nonphysiologic extremes of alkalinity or energy substrate deprivation. In the latter case, sperm achieved high rates of IVF. A shift in pH from 7.2 to 7.8 in a HEPES-buffered medium was sufficient to remove DEFB126 from the surface of most sperm after only 3 hr. The loss of DEFB126 from sperm under periovulaory fluid conditions has implications for the timing of release of sperm from the oviductal reservoir.

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Caffeine effects on meiotic maturation in hamster oocytes in vitro

Cited by 10 publications

References 33 publications

Effect of caffeine on meiotic maturation of porcine oocytes

Effect of caffeine on meiotic maturation of porcine oocytes

Effects of caffeine treatment on aged porcine oocytes: parthenogenetic activation ability, chromosome condensation and development to the blastocyst stage after somatic cell nuclear transfer

Release of DEFB126 from macaque sperm and completion of capacitation are triggered by conditions that simulate periovulatory oviductal fluid

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