cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection

Gómez, Eliana Rocio Rodríguez; Regino, William Otero; Monterrey, Pedro A.; Rangel, Alba Alicia Trespalacios

doi:10.1371/journal.pone.0227275

Cited by 10 publications

(10 citation statements)

References 36 publications

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“…[ 14 ] There is evidence in Western countries that the number of EPIYA motifs, especially section C, increased the risk of intestinal metaplasia and GC. [ 34 ] For instance, the frequency of strain rate exceeding one EPIYA-C (ABCC) replication was 51.1% in Colombia and 33.3% in Italy, which is much higher as compared to the present findings. [ 35 ] This would explain those countries as higher GC risk regions than Iran.…”

Section: Discussioncontrasting

confidence: 70%

EPIYA Motif Genetic Characterization from Helicobacter pylori Isolates in Distinct Geographical Regions of Iran

Moghim

Estaji

Esfahani

et al. 2022

Advanced Biomedical Research

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Background: This study aimed to determine the current EPIYA motifs of the cag A gene in Helicobacter pylori isolates from patients with gastric disorders, and evaluate the association between these patterns and the clinical outcome of H. pylori infection in different geographical regions of Iran. Materials and Methods: We examined 150 patients with gastrointestinal disorders from the central and eastern regions of Iran. The detection of H. pylori and screening of cag A was performed by polymerase chain reaction (PCR). The pattern of the motifs was determined by PCR followed by sequencing. Results: The overall prevalence of H. pylori was 66.3% in eastern (Mashad) and 50.6% in the central (Isfahan) part of Iran. The frequency of cag A-positive strains in Mashad and Isfahan were 63.4% and 56.7%, respectively. The pattern of EPIYA motif was as follows: 43 (79.6%) ABC, 7 (12.9%) AB, 4 (7.4%) ABCC, and one (1.9%) ABCCC. We also identified a novel EPIYA C sequence motif which showed association with gastric cancer (GC). The relationship between the frequency of specific EPIYA motifs and GC was statistically significant ( P < 0.05). Conclusions: This is the first report for the determination of the cag A EPIYA motif of H. pylori in the Northeast and center of Iran. The prevalence of cag A positive H. pylori between the two regions was significant ( P ≤ 0.05). All isolates of the H. pylori cag A were western type (ABC). The increase in the number of EPIYA-C repeats was associated with GC ( P ≤ 0.01).

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Section: Discussioncontrasting

confidence: 70%

EPIYA Motif Genetic Characterization from Helicobacter pylori Isolates in Distinct Geographical Regions of Iran

Moghim

Estaji

Esfahani

et al. 2022

Advanced Biomedical Research

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“…However in this study a significant correlation is seen between the number of EPIYA-C replicates and the age of the patients. The similarity of the prevalence and distribution of this motif in Iran and Colombia shows the same geographical distribution of this bacterium in the two countries and it can be concluded that the distribution in our study is mainly similar to the distribution in South America [45].…”

Section: The Association Between the Number Of The Epiya Motifs And The Icea2 Genesupporting

confidence: 76%

Determination of CagA EPIYA motif in Helicobacter pylori strains isolated from patients with digestive disorder

Khaledi

Bagheri

Validi

et al. 2020

Heliyon

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This study was conducted to identify patterns of cagA EPIYA motifs in H . pylori strains isolated from patients with gastrointestinal diseases in Hospitals of Shahrekord, and investigate the association between these biomarkers and clinical outcomes of gastrointestinal diseases due to H . pylori . In this study, 253 patients with gastrointestinal diseases were studied within 1395–1396. Histopathological investigations and urease test showed that 207 isolates were H . pylori -positive. Then, screening using a molecular technique, PCR, confirmed that 159 isolates had cagA. Finally, the pattern and prevalence of the motifs were determined by PCR and identified a number of motifs were sequenced. Results of this study showed that the pattern of motifs was as follows: ABC (140 isolates) (93/7%), ABCC (6 isolates) (3/77%), ABCCC (4 isolates) (2/5%), AB (7 isolates) (4/4%), AC (1 isolate) (0/6%), and BC (1 isolate) (0/6%). Sequencing results showed the presence of changed EPIYA motif in some isolates. CM motif sequence was also seen in all isolates. In this study, no significant association was seen between the prevalence rate of different patterns and clinical symptoms ( p = 0.71). There is a slight association between the presence of ABC motifs and the type of digestive disorder (p = 0.056). Results indicated that ABC was the most frequently seen pattern however, in such that positive cases of ABC motifs were more common in gastritis. All isolates had kinase phosphorylation region, and the observed pattern in this region was a generally western type (ABC).

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“…It has been demonstrated that EPIYA-D has a high affinity to bind to SHP-2 (pY-(V/T/A/I/S)-X-(L/I/V)-X-(F/W) (, whereas EPIYA-C has less affinity for SHP-2 (merely one amino acid in pY+5th position) compared with EPIYA-D [ 76 , 77 ]. However, according to literature, the presence of a higher number of EPI-X repeats is associated with the greater tendency of binding to SHP-2 [ 78 ]. Recently, in a study on the Mexican population, it was demonstrated that the risk of gastric cancer due to strains containing two or more repeats of EPIYA-C can be increased in infected patients [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although both ABCC and ABCCC genotypes are more prevalent in Iran in the occurrence of gastric ulcer and gastric cancer, according to our statistical analysis, only the ABCCC genotype was significantly associated with gastric cancer. Similarly, studies in South America, North America and Europe have shown that there is a significant relationship between infection by multiple EPIYA-C strains and gastric cancer in patients [ 74 , 78 ]. Interestingly, according to our results, it seems that infection by strains possessing the EPIYA-ABC motif has a preventive role against gastritis, peptic ulcer and gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

“…However, why is the number of gastric cancer cases in Iran as high as that in Japan, South Korea and China? It seems that the presence of the EPIYA-C motif, especially in strains with more than one repeat of EPIYA-C, can enhance the binding to SHP-2 and is considered a risk factor for gastric cancer [ 78 , 90 ]. According to our study, the presence of EPIYA-ABCCC was significantly related to gastric cancer in the Iranian population.…”

Section: Discussionmentioning

confidence: 99%

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EPIYA motifs of Helicobacter pylori cagA genotypes and gastrointestinal diseases in the Iranian population: a systematic review and meta-analysis

Keikha

2021

New Microbes and New Infections

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Helicobacter pylori is one of the best risk factors for gastric cancer. Recent studies have examined the relationship between virulence factors, in particular CagA toxin, and the development of gastrointestinal diseases. According to the literature, there is a significant relationship between the polymorphism of cagA -EPIYA motifs and progression to severe clinical outcomes. The main goal of our study was to determine the possible association between cagA genotypes and the risk of severe clinical outcomes in the Iranian population. We investigated these ambiguities using a comprehensive meta-analysis study, in which we evaluated data from 1762 Iranian patients for a potential correlation between all cagA gene genotypes and gastrointestinal diseases. According to statistical analysis, the frequencies of cagA genotypes including ABC, ABCC, AB and ABCCC in the Iranian population were estimated at 80.18%, 22.81%, 5.52% and 2.76%, respectively; the ABD genotype was not detected in these PCR-based studies. There was a significant relationship between cagA genotypes ABCC and ABCCC and severe clinical outcomes of infection such as peptic ulcer and gastric cancer. Overall, it can be concluded that there is a positive correlation with the number of copies of EPIYA-C and the increase of gastric cancer. Therefore, according to our results, it seems that the EPIYA-ABCCC motif has a strong positive relationship with gastric cancer in the Iranian population.

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cagA gene EPIYA motif genetic characterization from Colombian Helicobacter pylori isolates: Standardization of a molecular test for rapid clinical laboratory detection

Cited by 10 publications

References 36 publications

EPIYA Motif Genetic Characterization from Helicobacter pylori Isolates in Distinct Geographical Regions of Iran

EPIYA Motif Genetic Characterization from Helicobacter pylori Isolates in Distinct Geographical Regions of Iran

Determination of CagA EPIYA motif in Helicobacter pylori strains isolated from patients with digestive disorder

EPIYA motifs of Helicobacter pylori cagA genotypes and gastrointestinal diseases in the Iranian population: a systematic review and meta-analysis

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