Calbindin-D28k buffers intracellular calcium and promotes resistance to degeneration in PC12 cells

McMahon, Anne; Wong, Brendan S.; Iacopino, Anthony M.; Ng, May C.; Chi, Susan; German, Dwight C.

doi:10.1016/s0169-328x(97)00305-7

Cited by 89 publications

(58 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Notably, THexpressing neurons that also express CB and CR (Liang et al, 1996a;Nemoto et al, 1999;Gonzalez-Hernandez and Rodriguez, 2000) are known to be more resistant to 6Ј-OHDA and MPTP toxicity and are less affected in Parkinson's disease (Yamada et al, 1990;Liang et al, 1996b;Isaacs et al, 1997;McMahon et al, 1998;Damier et al, 1999a;Kim et al, 2000;Tsuboi et al, 2000) than DA neurons lacking expression of these Ca 2ϩ -binding proteins. CB and CR expression within the SNC of Wnt-BDNF KO mutant and control mice were analyzed.…”

Section: Wnt-bdnfmentioning

confidence: 99%

Brain-Derived Neurotrophic Factor Is Required for the Establishment of the Proper Number of Dopaminergic Neurons in the Substantia Nigra Pars Compacta

Baquet¹,

Bickford²,

Jones³

2005

J. Neurosci.

272

190

View full text Add to dashboard Cite

Brain-derived neurotrophic factor (BDNF) has been implicated in regulating neuronal survival, differentiation, and synaptic plasticity. Reduced expression of BDNF within the substantia nigra accompanies the deterioration of dopaminergic neurons in Parkinson's disease (PD) patients. Analysis of the effects of long-term BDNF absence from the CNS has been difficult because of the early postnatal lethality of BDNF Ϫ/Ϫ mice. Mice with a floxed BDNF allele were bred with Wnt1-Cre mice to generate Wnt-BDNF KO mice that lack BDNF from the midbrain-hindbrain (MHB). These mice are viable but exhibit hindlimb clutching and poor rotarod performance. Tyrosine hydroxylase (TH)-positive neuron numbers in the substantia nigra pars compacta (SNC) were estimated using stereological methods, revealing a persistent ϳ23% reduction of these cells at postnatal day 21 (P21) in Wnt-BDNF KO mice compared with controls. The diminishment of TH-expressing neurons was present at birth and continued through P120. This deficit appears selective for the dopaminergic population, because at P21, total neuron number within the SNC, defined as neuronal nuclei protein-positive cells, was not significantly reduced. Interestingly, and similar to observations in PD patients, SNC neuron subpopulations are not equally affected. Calbindin-and calretininexpressing SNC populations show no significant difference between Wnt-BDNF KO mice and controls. Thus, BDNF depletion from the MHB selectively leads to reduced TH expression in a subpopulation of neurons, but it remains unclear whether these cells are lost.

show abstract

Section: Wnt-bdnfmentioning

confidence: 99%

Brain-Derived Neurotrophic Factor Is Required for the Establishment of the Proper Number of Dopaminergic Neurons in the Substantia Nigra Pars Compacta

Baquet¹,

Bickford²,

Jones³

2005

J. Neurosci.

272

190

View full text Add to dashboard Cite

show abstract

“…protein is suggested by the finding that overexpression of calbindin decreased serum withdrawal-induced degeneration 8 and decreased the damage seen after hypoglycemic and glutamatergic challenge in vitro. 9,10 In vivo, calbindin overexpression decreased both excitotoxic and metabolic damage.…”

Section: +mentioning

confidence: 99%

Gene therapy effectiveness differs for neuronal survival and behavioral performance

et al. 2001

View full text Add to dashboard Cite

“…Calbindin-D 28k and other proteins (such as calretinin and parvalbumins) with calcium buffering capacities are reported to protect cells against calcium overload, and thereby from apoptosis. 40 For example, calbindin-D 28k expression interferes with the apoptotic pathway in lymphocytes, 41 osteoblastic cells, 42 neural cells, 43 pheochromocytoma PC-12 cells exposed to neurotoxin, 44 and C6 rat astrocytoma cells exposed to calcium ionophore 45 (for a review see Christakos et al 40 ). Calbindin-D 28k expression was also found in a subset of medulloblastomas presenting poorly differentiated cells but was absent in medulloblastomas with desmoplastic characteristics.…”

Section: Discussionmentioning

confidence: 99%

Calbindin‐D_28k

Pelc¹,

Vincent

Ruchoux

et al. 2002

Cancer

View full text Add to dashboard Cite

BACKGROUNDThe expression of the Ca2+‐binding protein calbindin‐D28k was analyzed in medulloblastomas in relation to clinical features and other biologic markers related to cell proliferation, differentiation, p53, and cerebellar developmental regulated gene expression.METHODSImmunohistochemistry was carried out on histologic slides from a first retrospective series of 29 nonmetastatic and 10 metastatic medulloblastoma formalin‐fixed, paraffin‐embedded tissues, using specific antibodies against calbindin‐D28k, calretinin, α‐parvalbumin and β‐parvalbumin, and S100 proteins. Informed consent was obtained from the subjects and/or guardians. Other biologic markers for differentiation, cell proliferation, the expression of the p53 tumor suppressor gene protein, and cerebellar developmental regulated genes were similarly investigated. A second series of 16 medulloblastomas from young patients (younger than 15 years) was added in order to validate the results obtained in the first series.RESULTSOf all the markers investigated, only calbindin‐D28k was significantly associated with prognosis. Survival and remission (i.e. recurrence free) time analysis performed on all the cases (n = 55) confirmed a high risk of death (P = 0.004) and recurrence (P = 0.003) associated with calbindin‐positivity. As calbindin‐positivity was predominantly observed in tumors from young patients, the authors confirmed its prognostic value in the subgroup of patients younger than 15 years (n = 37). Cox regression analysis showed a significant and independent prognostic value for calbindin expression and, to a lesser extent, the type of surgery (total or subtotal). Three risk groups were thus identified, distinguishing among the cases characterized by a total resection and calbindin‐negativity (good prognosis), by a subtotal resection and calbindin‐negativity (intermediary), and by calbindin‐positivity (bad prognosis).CONCLUSIONSThe current study suggests that calbindin‐positive medulloblastomas represent a subclass of aggressive tumors more frequently seen in younger patients. Cancer 2002;95:410–9. © 2002 American Cancer Society.DOI 10.1002/cncr.10666

show abstract

Calbindin-D28k buffers intracellular calcium and promotes resistance to degeneration in PC12 cells

Cited by 89 publications

References 27 publications

Brain-Derived Neurotrophic Factor Is Required for the Establishment of the Proper Number of Dopaminergic Neurons in the Substantia Nigra Pars Compacta

Brain-Derived Neurotrophic Factor Is Required for the Establishment of the Proper Number of Dopaminergic Neurons in the Substantia Nigra Pars Compacta

Gene therapy effectiveness differs for neuronal survival and behavioral performance

Calbindin‐D_28k

Contact Info

Product

Resources

About

Calbindin-D28k buffers intracellular calcium and promotes resistance to degeneration in PC12 cells

Cited by 89 publications

References 27 publications

Brain-Derived Neurotrophic Factor Is Required for the Establishment of the Proper Number of Dopaminergic Neurons in the Substantia Nigra Pars Compacta

Brain-Derived Neurotrophic Factor Is Required for the Establishment of the Proper Number of Dopaminergic Neurons in the Substantia Nigra Pars Compacta

Gene therapy effectiveness differs for neuronal survival and behavioral performance

Calbindin‐D28k

Contact Info

Product

Resources

About

Calbindin‐D_28k