Calcification in Pulmonary Heart Valve Tissue Engineering

Valk, Dewy C. van der; Fomina, Aleksandra; Uiterwijk, Marcelle; Hooijmans, Carlijn R.; Akiva, Anat; Kluin, Jolanda; Bouten, Cvc Carlijn; Smits, Anthal I.P.M.

doi:10.1016/j.jacbts.2022.09.009

Cited by 10 publications

(9 citation statements)

References 144 publications

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“…In support of this, a recent meta‐analysis on tissue‐engineered pulmonary valve calcification potential, reported an initial calcification surge within 1‐month after implantation, which then decreased between 1 and 3 months follow‐up. [ 62 ] However, when persistent activation of VICs occurs, due to insufficient valve design, material properties, or improper hemodynamical loading, maladaptive remodeling of the valve may also take place. Future studies may try to build on the current work to combine VICs and VECs and observe the potential resolution of calcific deposits.…”

Section: Discussionmentioning

confidence: 99%

Xenogeneic Serum‐Free Human Cell‐Derived Tissue Engineered Matrices for the Development of Clinical‐Grade Biomimetic Cardiovascular Devices

Zaytseva

Visser

Ehterami

et al. 2023

Advanced Therapeutics

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The development of next‐generation biomimetic cardiovascular implants using tissue engineering concepts can address the existing shortcomings of the clinically available prostheses, offering the possibility to generate life‐long, native‐analogous constructs with self‐remodeling and regenerative capacities. Scaffolds for tissue‐engineered cardiovascular prostheses can be obtained from allogenic cell sources, that can then produce human tissue‐engineered matrices (hTEMs) in vitro. Traditionally, fetal bovine serum (FBS) is used as a universal cell growth supplement. However, concerns regarding its biosafety remain a challenge for clinical translation. The aim of this study is to develop a novel xenogeneic serum‐free approach for the manufacturing of clinical grade hTEMs. To achieve this, decellularized hTEMs are generated under xenogeneic serum‐free conditions and have subsequently demonstrated hTEMs perform similarly to the FBS‐supplemented control group in terms of extracellular matrix (ECM) composition, hemocompatibility, thrombogenicity, and calcification potential. Finally, the xenogeneic serum‐free protocol is successfully adapted to the development of hTEM‐based tissue‐engineered heart valves for the systemic circulation, showing proof‐of‐concept functionality in vitro. Overall, the data suggest the effectiveness of xenogeneic serum‐free culture method as a valid alternative to FBS for the production of hTEM for cardiovascular applications.

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Section: Discussionmentioning

confidence: 99%

Xenogeneic Serum‐Free Human Cell‐Derived Tissue Engineered Matrices for the Development of Clinical‐Grade Biomimetic Cardiovascular Devices

Zaytseva

Visser

Ehterami

et al. 2023

Advanced Therapeutics

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show abstract

“…Second, the calcification scoring system applied appears equivocal, as it subsumes the authors’ own analysis of published images and, in its absence, the description provided by the authors of the respective papers. 1 Supporting the concern of interpretation variance, the calcification example in Figure 2 in the paper may well qualify as microcalcification rather than mild calcification, as described in the original publication.…”

mentioning

confidence: 82%

“…Van der Valk et al 1 assessed calcification in pulmonary tissue-engineered heart-valves (TEHVs) and reported occurrence in 35% of large animal implants mainly on a mild to microcalcific level. Further subgroup analyses showed no significant differences.…”

mentioning

confidence: 99%

“…Yet, although only 35% of implants presented microspots to mild calcification, the value of this meta-analysis lies in creating awareness for the end goal of tissue engineering beyond functional living leaflets, which is the avoidance of calcific degeneration. While awareness for using “true” calcification models may follow in the wake, Van der Valk et al 1 also highlighted the need for standardized classification of tissue-engineered approaches.…”

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confidence: 99%

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