Calcitonin-Derived Carrier Peptides

Neundorf, Ines; Ag, Beck-Sickinger

doi:10.2174/138161205774580723

Cited by 6 publications

(3 citation statements)

References 51 publications

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“…Owing to a high permeability through the nasal epithelium, it is applied as an intranasal therapeutic . Structure−activity studies revealed that the truncated sequence hCT(9−32) can translocate through cell membranes, although the calcitonin receptor activating N-terminus (aa 1−8) of hCT is lacking , . By using hCT(9−32), efficient transport of different cargoes into cells like the antiproliferative drug daunorubicin, fluorophors, or even large proteins has been achieved , .…”

Section: Introductionmentioning

confidence: 99%

Quantum Dot−Carrier Peptide Conjugates Suitable for Imaging and Delivery Applications

et al. 2008

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We developed multifunctional fluorescent nanoparticles suitable for the nonviral delivery of negatively charged molecules like RNA. Therefore, we incorporated the recently developed branched hCT-derived carrier peptide hCT(18-32)-k7 on the surface of luminescent quantum dots (QDs). Besides detailed characterization of our QD-peptide conjugates concerning stability, toxicity, and uptake mechanism. we used them for efficient RNA delivery into different cell lines. The results of our studies indicate the involvement of more than one endocytotic uptake pathway in the internalization process. Furthermore, we could show that the QD-peptide bioconjugates exhibit no effect on cell viability and possess high stability inside living cells. The efficacy of our newly designed constructs for oligonucleotide drug delivery is highlighted by the successful intracellular transport of Cy-3 labeled RNA. Moreover, by using the chemotherapeutic chloroquine the efficient release of the assemblies out of endosomes was demonstrated. These results prove that our multifunctional platforms are versatile tools for diagnostic and therapeutic imaging purposes applicable for biologically active siRNA or aptamer sequences.

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Section: Introductionmentioning

confidence: 99%

Quantum Dot−Carrier Peptide Conjugates Suitable for Imaging and Delivery Applications

et al. 2008

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“…9 Recently, truncated sequences of the human peptide hormone calcitonin (hCT) were successfully applied as delivery vehicle for different substances, like proteins, oligonucleotides, and small organic molecules. 10 Since then, very promising branched structures have been developed and have served for the delivery of plasmid DNA as well as nanoparticles. 11,12 The side sequence of these branched peptides contains positive charged amino acids for complexation of negatively charged DNA or RNA molecules.…”

Section: Introductionmentioning

confidence: 99%

Specific labeling with potent radiolabels alters the uptake of cell‐penetrating peptides

et al. 2009

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Radiolabeled peptides play an important role in radiopharmacy not only as tumor markers but also as transport vectors. Therefore, cell-penetrating peptides (CPP) may serve as very effective delivery tools, as well. Recently, CPP based on the human hormone calcitonin (hCT) have been developed. Especially, branched hCT-peptide sequences turned out to have highly efficient internalization capacities. Labeling these peptides with radionuclides would generate promising new tools for imaging and therapy applications in radiopharmacy. However, the influence of the metal complexation on the internalization capacity of CPP has not been elucidated yet in detail. In this study we quantified the uptake of Ga-DOTA modified hCT-carrier peptides in HeLa cells by using atomic absorption spectroscopy (AAS) and compared the results to the uptake of fluorescently-labeled peptides. Interestingly, we measured different uptake rates depending on the attached label. Unexpectedly, modification with a Ga-DOTA complex can have tremendous effects on the uptake efficiency. The results of these studies support the need of detailed analysis of each carrier peptide/cargo construct, especially in the field of metal complex modified CPP.

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“…Recently, CPPs based on the human calcitonin have been described . Calcitonin is a 32 amino acid long peptide hormone that is physiologically secreted by the thyroid gland and involved in the regulation of the calcium homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Cyclization of a cell‐penetrating peptide via click‐chemistry increases proteolytic resistance and improves drug delivery

Reichart

Horn

Neundorf

2016

Journal of Peptide Science

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In this work we report synthesis and biological evaluation of a cell-penetrating peptide (CPP), that is partly cyclized via a triazole bridge. Recently, beneficious properties have been reported for cyclized peptides concerning their metabolic stability and intracellular uptake. A CPP based on human calcitonin was used in this study, and side chain cyclization was achieved via copper catalyzed alkyne-azide click reaction. Cell viability studies in several cell-lines revealed no cytotoxic effects. Furthermore, efficient uptake in breast cancer MCF-7 cells could be determined. Moreover, preliminary studies using this novel peptide as drug transporter for daunorubicin were performed. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd.

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Calcitonin-Derived Carrier Peptides

Cited by 6 publications

References 51 publications

Quantum Dot−Carrier Peptide Conjugates Suitable for Imaging and Delivery Applications

Quantum Dot−Carrier Peptide Conjugates Suitable for Imaging and Delivery Applications

Specific labeling with potent radiolabels alters the uptake of cell‐penetrating peptides

Cyclization of a cell‐penetrating peptide via click‐chemistry increases proteolytic resistance and improves drug delivery

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