Calcium absorption and calcium-binding protein in chicks on differing calcium and phosphorus intakes

Morrissey, R.L.; Wasserman, R H

doi:10.1152/ajplegacy.1971.220.5.1509

Cited by 209 publications

(64 citation statements)

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“…In an in-vitro trial, using ligated duodenal loops, Morrissey & Wasserman (1971) observed that broiler chicks absorbed a higher percent of a labeled 47 Ca (ranging from 70 to 90%) when diets low in Ca (0.08%) were fed for eight d prior to intestinal sampling regardless of dietary P levels, or when low P (0.25%) diets were fed regardless of dietary Ca levels. Chickens receiving a diet with normal P (0.65%) and normal Ca (1.20%) absorbed less than 50% of 47 Ca.…”

Section: Early Dietary Adaptationmentioning

confidence: 99%

Nutritional genomics: a practical approach by early life conditioning with dietary phosphorus

Ashwell¹,

Angel

2010

R. Bras. Zootec.

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-The recent technologies that have led to the new field of functional genomics (how the genome of an organism regulates homeostasis and responds to stimuli) are providing a clearer understanding of how organisms interact with their environment and in particular their diet. We are beginning to learn how the diet may have long-term influence on performance and health. A form of epigenetic regulation has been recently described called fetal "programming". Fueled by epidemiological data the "fetal origins" hypothesis suggests that a poor in utero environment resulting from maternal dietary or placental insufficiency may "program" susceptibility in the fetus to cardiovascular or metabolic disorders. We have observed similar apparent programming by dietary manipulation in the chicken. When birds are challenged with a diet low in phosphorus (P) for 90 hours post-hatch they obtain the ability to better utilize P later in life. This increased retention of P from the diet can partially be explained by an enduring increase in the expression of the intestine-specific Na/P cotransporter (NaPcoT) gene during programming as well as later in life when fed P restricted diets. The resulting data provide the first evidence for neonatal programming of gene expression in an oviparous species.Key Words: epigenetics, functional genoimcs, nutrition, nutritional conditioning, poultry Genômica nutricional: uma abordagem prática pelo condicionamento com fósforo dietético nos primeiros anos de vida RESUMO -As tecnologias recentes que levaram ao novo campo da genômica funcional (como o genoma de um organismo regula a homeostase e responde aos estímulos) estão proporcionando uma compreensão mais clara de como os organismos interagem com seu ambiente e em especial a sua dieta. Nós estamos começando a aprender como a dieta pode ter influência a longo prazo sobre o desempenho e a saúde. Uma forma de regulação epigenética foi descrita recentemente chamada de "programação fetal". Alimentados por dados epidemiológicos da "origem fetal" hipótese sugere que um pobre no útero ambiente decorrentes da insuficiência alimentar ou placentária materna pode programar "suscetibilidade do feto de doenças cardiovasculares ou metabólicas. Tem-se observado programação aparente similar por manipulação dietética da galinha. Quando as aves são desafiadas com uma dieta baixa em fósforo (P) por 90 horas pós-eclosão, elas obtêm a capacidade de utilizar P melhor mais tarde na vida. Esta maior retenção de P da dieta pode ser parcialmente explicada por um aumento permanente na expressão do gene do cotransoprtador Na/P intestinal durante a programação, bem como mais tarde na vida quando alimentadas com dietas P restrito. Os dados obtidos fornecem a primeira evidência para a programação neonatal da expressão gênica em uma espécie ovípara.

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Section: Early Dietary Adaptationmentioning

confidence: 99%

Nutritional genomics: a practical approach by early life conditioning with dietary phosphorus

Ashwell¹,

Angel

2010

R. Bras. Zootec.

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“…Ca absorption was estimated by an already described modification (Bonjour et al 1973a) of the in situ ligated loop method (Wasserman, 1963;Morrissey & Wasserman, 1971). The animals were anaesthetized with an i.P.…”

Section: Measurement Of Ca Absorptionmentioning

confidence: 99%

Calcium absorption in diphosphonate‐treated rats: effect of parathyroid function, dietary calcium and phosphorus.

Bonjour¹,

Fleisch²,

Trechsel³

1977

The Journal of Physiology

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SUMMARY1. The role of parathyroid hormone (PTH) and 1,25-dihydroxycholecalciferol (1,25-(OH)2D3) in modulation of intestinal Ca absorption was studied in rats, using disodium ethane-l-hydroxy-l,1-diphosphonate (EHDP), which is known to reduce 1,25-(OH)2D3 formation.2. EHDP decreased intestinal Ca absorption. This effect could be abolished by small amounts of 1,25-(OH)2D3, whereas even large doses of PTH were ineffective. EHDP also decreased Ca absorption in thyroparathyroidectomized (TPTX) rats. Therefore the effect of EHDP on 1,25-(OH)2D3 production is unlikely to be mediated through PTH.3. The correction by PTH of the decreased Ca absorption in TPTX rats was inhibited by EHDP. Since EHDP inhibits formation of 1,25-(OH)2D3 the effect of PTH on Ca absorption is likely to be mediated through this vitamin D3 metabolite. 4. In normal rats both a low Ca and a low P diet stimulated Ca absorption. In EHDP-treated intact rats low Ca still stimulated Ca absorption, whereas the effect of low P was abolished. This indicates that low Ca and low P diets affect Ca absorption through different mechanisms.5. Intestinal adaptation to a low Ca diet was still observed in EHDPtreated TPTX rats. Thus, in the rat, intestinal adaptation to low Ca diet can occur without PTH.

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“…Further, animals fed diets deficient in calcium or phosphorus adapt to these deficiencies by an increase in the synthesis of 1,25(0H)2D3 (17)(18)(19)(20)(21), resulting in an increase in the efficiency of calcium and phosphorus absorption. These adaptations are associated with an increased synthesis of calbindin-D and its mRNA (17)(18)(19)(20)(22)(23)(24)(25)(26)(27)(28) …”

Section: Introductionmentioning

confidence: 99%

Vitamin D and adaptation to dietary calcium and phosphate deficiencies increase intestinal plasma membrane calcium pump gene expression.

Cai

Chandler

Wasserman

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

157

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The effect of vitamin D and other variables on the synthesis of the chicken intestinal plasma membrane calcium pump (PMCA) mRNA was assesed. The DNA probe for Northern analysis was obtained by reverse transcription and PCR with intestinal poly(A)+ RNA, using two 20-mer oligonucleotide primers homologous to the 3' coding region of the human teratoma PMCA. An EcoRI restriction fragent of the PCR product was cloned into the pBluescript I KS(-) phagemid vector, and the chimeric plasmid was used to transform Escherichia coli. The amino acid sequence deduced from the nucleotide DNA sequence of the PCR product and the cloned DNA were 96% homologous with the teratoma sequence. Northern blots of intestinal poly(A)+ RNA with 32P-labeled DNA showed the presence of three major species of chicken PMCA mRNAs at about 6.6, 5.4, and 4.5 kb. Northern analysis with the chicken PMCA DNA indicated that repletion of vitamin D-deficient chickens with vitamin D increased PMCA mRNAs in the duodenum, jejunum, ileum, and colon. After injection of 1,25-dihydroxyvitamin D3 intravenously into vitamin D-deflcient chickens, duodenal PMCA mRNA tended to increase by 2 hr, reached a maximum at about 16 hr, and returned to baseline levels at 48 hr. Adaptation of chickens to either a calcium-or phosphorus-deficient diet resulted in a 2-to 3-fold increase in duodenal PMCA mRNA. These results indicate that vitamin D and specific variables that affect calcium absorption through the vitamin D-endocrine system increase intestinal PMCA gene expression.A major factor controlling the efficiency of calcium absorption is the vitamin D hormone, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] (1, 2). 1,25(OH)2D3 elicits both genomic (3) and nongenomic (4-10) effects on the intestinal cell. Among the former effects are the induction of the synthesis of intestinal calbindin-D9k in mammalian species and intestinal calbindin-D28k in avian species (3). In addition, we recently demonstrated another apparent genomic response, a vitamin D-dependent increase in the amount ofcalcium pump units in the basolateral membrane of the avian enterocyte (11). This finding is consistent with previous observations (12)(13)(14)(15)(16) showing that repletion of vitamin D-deficient animals with vitamin D or 1,25(OH)2D3 results in an enhancement of the ATP-dependent uptake of Ca2+ by isolated basolateral membrane vesicles. Further, animals fed diets deficient in calcium or phosphorus adapt to these deficiencies by an increase in the synthesis of 1,25(0H)2D3 (17)(18)(19)(20)(21), resulting in an increase in the efficiency of calcium and phosphorus absorption. These adaptations are associated with an increased synthesis of calbindin-D and its mRNA (17)(18)(19)(20)(22)(23)(24)(25)(26)(27)(28)

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Calcium absorption and calcium-binding protein in chicks on differing calcium and phosphorus intakes

Cited by 209 publications

References 0 publications

Nutritional genomics: a practical approach by early life conditioning with dietary phosphorus

Nutritional genomics: a practical approach by early life conditioning with dietary phosphorus

Calcium absorption in diphosphonate‐treated rats: effect of parathyroid function, dietary calcium and phosphorus.

Vitamin D and adaptation to dietary calcium and phosphate deficiencies increase intestinal plasma membrane calcium pump gene expression.

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