Calcium Carbide-Induced Haematological Alterations In The Albino Mice- Mus musculus

Ogbuagu, Dike Henry; Ujowundu, C. O.; Izunobi, Lucy Chinomso

doi:10.9790/2402-101001100104

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…Leukopenia in mice administered with CaC 2 is indicative of CaC 2 -induced severe suppression of lymphoproliferative processes. Similar findings have been reported in other related studies [11]. Notably, other studies have shown contradictory findings in rats fed on fruits ripened with CaC 2 [10].…”

Section: Discussionsupporting

confidence: 91%

“…Decrease in PCV may be attributed to harmful effect of calcium carbide on the bone marrow, alteration of micronutrients for RBC synthesis, inhibition of erythropoietin and elevated haemolysis of the red cells due to noxious consequence. A similar study in which mice were fed on fruits ripened by CaC 2 showed reduced PCV levels [11]. Indeed, a decline in PCV is one of the key features of exposure to toxic chemicals that is associated with generation of ROS and lipid peroxidation [16].…”

Section: Discussionmentioning

confidence: 95%

“…It is well established that CaC 2 impacts the sensory system by limiting oxygen supply to the brain, with potential consequence of neural damage. In addition, evidence shows involvement in lung failure, renal failure, dermal diseases and heart conditions [10,11]. There is a need for detailed studies to elucidate and characterize the negative physiological and biochemical process affected by CaC 2 in humans to aid in diagnosing exposure and development of pharmaceutical intervention.…”

Section: Introductionmentioning

confidence: 99%

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Calcium carbide–induced derangement of hematopoiesis and organ toxicity ameliorated by cyanocobalamin in a mouse model

et al. 2022

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Background Calcium carbide (CaC2) is a chemical primarily used in the production of acetylene gas. The misuse of CaC2 to induce fruit ripening is a global challenge with a potential adverse effects to human health. Additionally, CaC2 is known to contain some reasonable amount of arsenic and phosphorous compounds that are toxic and pose a danger to human health when ingested. The current study sought to characterize CaC2 toxicity and elucidate any protective effects by cyanocobalamin (vitamin B12), a well-established antioxidant and anti-inflammatory bio-molecule. Female Swiss white mice were randomly assigned into three groups; the first group was the control, while the second group was administered with CaC2. The third group received CaC2 followed by administration of vitamin B12. The mice were sacrificed at 60 days post treatment, hematological, biochemical, glutathione assay, cytokine ELISA and standard histopathology was performed. Results CaC2 administration did not significantly alter the mice body weight. CaC2 administration resulted in a significant decrease in packed cell volume (PCV), hemoglobin (Hb), red blood cells (RBCs) and RBC indices; indicative of CaC2-driven normochromic microcytic anaemia. Further analysis showed CaC2-driven leukopenia. Evidently, vitamin B12 blocked CaC2-driven suppression of PCV, Hb, RBCs and WBCs. Monocytes and neutrophils were significantly up-regulated by CaC2. CaC2-induced elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and bilirubin signaled significant liver damage. Notably, vitamin B12 stabilized AST, ALT and bilirubin in the presence of CaC2, an indication of a protective effect. Histopathological analysis depicted that vitamin B12 ameliorated CaC2-driven liver and kidney injury. CaC2 resulted in the depletion of glutathione (GSH) levels in the liver; while in the brain, kidney and lungs, the GSH levels were elevated. CaC2 administration resulted in elevation of pro-inflammatory cytokines TNF-α and IFN-γ. Vitamin B12 assuaged the CaC2-induced elevation of these pro-inflammatory cytokines. Conclusions These findings demonstrate for the first time that oral supplementation with vitamin B12 can protect mice against CaC2-mediated toxicity, inflammation and oxidative stress. The findings provide vital tools for forensic and diagnostic indicators for harmful CaC2 exposure; while providing useful insights into how vitamin B12 can be explored further as an adjunct therapy for CaC2 toxicity.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Calcium carbide–induced derangement of hematopoiesis and organ toxicity ameliorated by cyanocobalamin in a mouse model

et al. 2022

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“…Few laboratorial studies have also reported the toxicological effects associated with consumption of calcium carbide-ripened fruits that include changes in some hormonal parameters, oxidative stress enzymes, haematological indices, and morphologies of ovaries and testes in rats [15,16,17,18,19]. These studies also reported that calcium carbide-ripened fruits could accelerate puberty onset as well as reduce sperm count.…”

Section: Introductionmentioning

confidence: 99%

Alterations in Some Haematological and Histological Parameters on Consumption of Calcium Carbide-Ripened Banana in Male Albino Mice

Kalita,

Hazarika

2023

UPJOZ

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The aim of this study was to investigate the changes in several haematological and histopathological parameters in male Swiss albino mice fed on diets with calcium carbide-ripened banana. Fruits were collected from Khahigarh village, located in Raha subdivision of Nagaon district in Assam, and were artificially ripened using calcium carbide. The calcium carbide ripened banana was fed to albino mice, two times in a day for 14 consecutive days. Naturally ripened banana was used as positive control, and treated same way in another group of mice. The results showed that calcium carbide ripened banana induced significant decrease in RBCs count and Haemoglobin concentration; and significant increase in the WBCs. Histopathological observations of liver, kidney and testes also revealed the hazardous effect of the chemical. However, no genotoxic effect was observed with the chemical. The results suggest that consumption of banana ripened with calcium carbide can lower the body’s ability to resist some alterations of normal functions.

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The use of calcium carbide in food and fruit ripening: Potential mechanisms of toxicity to humans and future prospects

et al. 2022

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Calcium Carbide-Induced Haematological Alterations In The Albino Mice- Mus musculus

Cited by 8 publications

References 12 publications

Calcium carbide–induced derangement of hematopoiesis and organ toxicity ameliorated by cyanocobalamin in a mouse model

Calcium carbide–induced derangement of hematopoiesis and organ toxicity ameliorated by cyanocobalamin in a mouse model

Alterations in Some Haematological and Histological Parameters on Consumption of Calcium Carbide-Ripened Banana in Male Albino Mice

The use of calcium carbide in food and fruit ripening: Potential mechanisms of toxicity to humans and future prospects

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