Calcium Carbonate Nanoparticles: Synthesis,Characterization and Biocompatibility

Abstract

The synthesis of nanoparticles and their functionalization to effectively utilize them in biological applications including drug delivery is currently a challenge. Calcium carbonate among many other inorganic nanosized particles offers promising results for such applications. We have synthesized calcium carbonate nanoparticles using polymer mediated growth technique, where one of the ions bound within polymer matrix and the other diffuses and reacts to form desired compound. The synthesized nanoparticles are characterized using X-ray diffraction, Scanning Electron Microscopy and spectroscopic techniques such as Fourier-Transform Infra-red spectroscopy and UV-Vis spectroscopy. The diameter of the calcium carbonate nanoparticles is estimated to be 39.8 nm and their biocompatibility studies showed no significant induction of oxidative stress or cell death even at higher concentrations (50 microg) upon exposure to HeLa and LE cells. Here, we report that the synthesized calcium carbonate nanosized particles using polymer mediated growth technique are biocompatible and can be safely used for biomedical applications.

3D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivo

“…The aim of this paper is to use, for the first time, 3D chitin scaffolds isolated from the giant verongiid demosponge A. archeri ( Figure 1) for molluscs hemolymph-associated biomineralization ex vivo. This experimental study was additionally motivated by the fact that both amorphous and crystalline phases (i.e., calcite) of calcium carbonates are known to be bio-compatible [87][88][89], biodegradable [90][91][92] and osteoinductive substrates for tissue engineering of bones and other hard tissues [93][94][95][96][97][98][99][100][101][102][103][104][105].…”

Progressive slowdown/prevention of cellular senescence by CD9-targeted delivery of rapamycin using lactose-wrapped calcium carbonate nanoparticles

“…Therefore, this concentration was used in further experiments. Rapa loading can be ascribed to the porous structure of the NPs that prevented any alterations in the particle sizes of NPs after drug loading17.…”

Cockle Shell-Derived Calcium Carbonate (Aragonite) Nanoparticles: A Dynamite to Nanomedicine

“…Nanoparticles have the ability to cross the brain barriers especially the blood brain barrier thereby shielding the encapsulated therapeutic agent and efficiently delivering them to the targeted areas, thus, it is considered suitable for drug delivery systems for the treatment of neurodegenerative diseases [38]. Nanomaterials are indeed very effective and have potential abilities to alter and modify a drug's mechanisms of action, bone reinforcement, DNA structure probes, tissue engineering, drug and gene delivery, protein detection and tumor destruction, molecular imaging, diagnostics, regenerative medicine, pharmaceutical sciences, engineering and industrial chemistry [39].…”

“…CaCO 3 in aragonite crystals form differs from calcite by its nature of occurrence, the aragonite exists in an orthorhombic system with crystals which results in the formation of pseudo-hexagonal crystals due to continuous twinning [9]. In addition, the aragonite polymorph is more dense than calcite and so can be unravelled, synthesized and replaced by bones [15] however, aragonite has been applicable in tissue engineering, bone repairs and anticancer drug carrier [39].…”