Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells

Pei, Zhe; Murata, Yoshiyuki; Benning, Gregor; Thomine, Sébastien; Klüsener, Birgit; Allen, Grant; Grill, Erwin; Schroeder, Julian

doi:10.1038/35021067

Cited by 1,908 publications

(1,929 citation statements)

References 32 publications

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“…Intracellular Ca 2+ increases were reported to be a consequence of an uptake from the extracellular space or due to Ca 2+ liberation from intracellular stores. Therefore, NO Results of the present study show that NO and H 2 O 2 mediate SO 2 toxicity via Ca 2+ -dependent pathway, which is consistent with previous reports showing that NO and H 2 O 2 act via Ca 2+ signaling pathways in plants responding to abiotic stress (Pei et al 2000;Qiao and Fan 2014;Niu and Liao 2016). These results will broaden our understanding on how plants respond to environmental stress.…”

Section: Discussionsupporting

confidence: 92%

“…When [Ca 2+ ] cyt elevation in guard cells was blocked by LaCl 3 , SO 2 toxicity was blocked. Furthermore, suppression of the elevation of NO (Pei et al 2000;Lamotte et al 2006); meanwhile, NO also selectively activates intracellular Ca 2+ channels via a cGMPdependent pathway in V. faba guard cells (Garcia-Mata et al 2003). Intracellular Ca 2+ increases were reported to be a consequence of an uptake from the extracellular space or due to Ca 2+ liberation from intracellular stores.…”

Section: Discussionmentioning

confidence: 99%

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NO and H2O2 contribute to SO2 toxicity via Ca2+ signaling in Vicia faba guard cells

Bai

Xue

et al. 2017

Environ Sci Pollut Res

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NO and H 2 O 2 have been implicated as important signals in biotic and abiotic stress responses of plants to the environment. Previously, we have shown that SO 2 exposure increased the levels of NO and H 2 O 2 in plant cells. We hypothesize that, as signaling molecules, NO and H 2 O 2 mediate SO 2 -caused toxicity. In this paper, we show that SO 2 hydrates caused guard cell death in a concentration-dependent manner in the concentration range of 0.25 to 6 mmol L −1 , which was associated with elevation of intracellular NO, H 2 O 2 , and Ca 2+ levels in Vicia faba guard cells. NO donor SNP enhanced SO 2 toxicity, while NO scavenger c-PTIO and NO synthesis inhibitors L-NAME and tungstate significantly prevented SO 2 toxicity. ROS scavenger ascorbic acid (AsA) and catalase (CAT), Ca 2+ chelating agent EGTA, and Ca 2+ channel inhibitor LaCl 3 also markedly blocked SO 2 toxicity. In addition, both c-PTIO and AsA could completely block SO 2 -induced elevation of intracellular Ca 2+ level. Moreover, c-PTIO efficiently blocked SO 2 -induced H 2 O 2 elevation, and AsA significantly blocked SO 2 -induced NO elevation. These results indicate that extra NO and H 2 O 2 are produced and accumulated in SO 2 -treated guard cells, which further activate Ca 2+ signaling to mediate SO 2 toxicity. Our findings suggest that both NO and H 2 O 2 contribute to SO 2 toxicity via Ca 2+ signaling.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

NO and H2O2 contribute to SO2 toxicity via Ca2+ signaling in Vicia faba guard cells

Bai

Xue

et al. 2017

Environ Sci Pollut Res

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“…These results indicate that MYB30 most likely acts independent of auxin and cytokinin in root growth but MYB30 plays some regulatory roles in root growth under ABAs signaling. ABA is known to induce ROS cellular level in guard cells and the mitochondria at the root tip [12,15]. In addition, MYB30 is reported to be involved in seed germination under ABA signaling [17].…”

Section: Interaction Between Myb30 and Plant Hormones In Root Growthmentioning

confidence: 99%

“…However, further analyses are still required to elucidate the relationship between ROS and plant hormones. Especially, ABA is known as a regulator of ROS production under abiotic stresses [12,14,15]. In this study, we analyzed the relationship between MYB30 gene regulatory network and plant hormones such as auxin, cytokinin, and ABA.…”

mentioning

confidence: 99%

MYB30 regulates root cell elongation under abscisic acid signaling

Sakaoka

Mabuchi

Morikami

et al. 2018

Communicative & Integrative Biology

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Reactive oxygen species (ROS) and plant hormones play important roles in regulating plant growth and stress responses as signaling molecules. Abscisic acid (ABA) is known as the key regulator of both abiotic and biotic stress responses. During stress responses, ABA is known to regulate ROS production, indicating that important crosstalk occurs between ROS and ABA signaling. We recently reported that MYB30, an MYB-type transcription factor, regulates root cell elongation under ROS signaling. In this study, we analyzed the molecular interaction between ROS and ABA signal during for root development, which is mediated through MYB30 transcriptional regulation. We showed that MYB30-regulated root cell elongation was mediated by ROS production under ABA signaling. Our findings will provide one piece of evidence of the complex cross talk between ROS and hormone signaling that regulates root development.

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“…Blocking H 2 O 2 production in guard cells inhibits ABA-induced stomatal closure emphasizing the role of ROS during this process [117]. The PM ABA-activated Ca 2+ currents of guard cells are likewise activated by H 2 O 2 [116,117].…”

Section: Ca 2+ Channels: Roles In Guard Cell Signal Transductionmentioning

confidence: 99%

Roles of ion channels and transporters in guard cell signal transduction

2007

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Stomatal complexes consist of pairs of guard cells and the pore they enclose. Reversible changes in guard cell volume alter the aperture of the pore and provide the major regulatory mechanism for control of gas exchange between the plant and the environment. Stomatal movement is facilitated by the activity of ion channels and ion transporters found in the plasma membrane and vacuolar membrane of guard cells. Progress in recent years has elucidated the molecular identities of many guard cell transport proteins, and described their modulation by various cellular signal transduction components during stomatal opening and closure prompted by environmental and endogenous stimuli.

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Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells

Cited by 1,908 publications

References 32 publications

NO and H2O2 contribute to SO2 toxicity via Ca2+ signaling in Vicia faba guard cells

NO and H2O2 contribute to SO2 toxicity via Ca2+ signaling in Vicia faba guard cells

MYB30 regulates root cell elongation under abscisic acid signaling

Roles of ion channels and transporters in guard cell signal transduction

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