Calcium-controlled peroxidase secretion by sugarbeet cell suspensions in relation to habituation

Kevers, Claire; Sticher, Liliane; Penel, Claude; Greppin, Hubert; Gaspar, Th.

doi:10.1007/bf00024222

Cited by 31 publications

(7 citation statements)

References 20 publications

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“…One would expect a strong decrease in activity of all isoenzymes due to dilution with fresh medium, as observed in several other species (ARNISON & BOLL 1976b, BREDEMEIJER et al 1985. Possibly, the decrease in activity caused by dilution was for certain peroxidase isoenzymes compensated by a rapid release from the cells, for example by a higher level of calcium in fresh medium (KEVERS et al 1982). The decrease in activity of the cellular fraction of isoenzyme B2 caused by subculture supports this assumption.…”

Section: Discussionmentioning

confidence: 59%

Peroxidase in Suspension Cultures of Haplopappus Gracilis

Bredemeijer¹,

Burg²

1986

Acta Botanica Neerlandica

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Peroxidase activity and isoenzyme composition of cells and culture medium were analysed in a cell suspension culture of Haplopappus gracilis. The results revealed marked changes in the activity and isoenzyme patterns of peroxidase during the culture cycle. During the exponential growth phase the peroxidase activity of the cells increased and a considerable amount of peroxidase was released into the culture medium. Several peroxidase isoenzymes in the medium increased in activity continuously throughout the whole exponential growth phase while others decreased during the second half of this phase. The decrease of the latter was caused by diffusion of the isoenzymes back into the cell walls as well as by inhibitors. During the stationary phase of the cell cycle only small changes occurred in the peroxidase activity and isoenzyme patterns.

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Section: Discussionmentioning

confidence: 59%

Peroxidase in Suspension Cultures of Haplopappus Gracilis

Bredemeijer¹,

Burg²

1986

Acta Botanica Neerlandica

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“…It has also been noted that POD release from cultured cells of various plant species was regulated by calcium/calmodulin (Kevers et al, 1982;Sticher et al, 1981;Xu et al, 1993). Whether or not the induction of POD by Fe 2+ is also mediated by calcium/calmodulin was investigated here.…”

Section: Discussionmentioning

confidence: 99%

Induction of peroxidase by Fe2+ in detached rice leaves

Peng

et al. 1996

Plant Soil

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Peroxidase (POD) activity was significantly stimulated in detached rice leaves by Fe 2+. By electric focusing with thin-layer scanning, three new bands were identified in Fe2+-treated leaves, and the activities of other isozymes were also drastically increased, of which a band with a pI of ca. 9.8 was enhanced from trace amount (invisible) in the control to a large band in the FeZ+-treated leaves. How Fe z+ induced POD activity was investigated. Cycloheximide completely blocked the stimulation of POD activity by Fe 2+, but actinomycin D could not inhibit it effectively, indicating that the stimulated activity resulted from inducing de novo biosynthesis of the enzyme at translational level rather than at transcriptional level. When same molar concentration of H202 as Fe 2+ and sublethal and lethal concentrations of paraquat were introduced to the rice leaves, no significant stimulation of POD activity was observed compared with Fe 2+-induced activity, and scavengers of free radicals still could not inhibit the induction of POD by Fe 2+. Several chemicals such as EGTA, LaC13, CPZ, verapamil, which were known to interfere with the action of calcium/calmodulin, were not able to retard POD induction by Fe z+. From these results it appears that the process of Fe2+-induced POD may not be mediated by free radicals or calciundcalmodulin action.

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“…The predominant part of the peroxidase in the medium appears to be the result of an active secretion (STICHER et al 1981;KEVERS et al 1982; VAN HUYSTEE & LoBARZEWSKI 1982). Further, it is known that the release of peroxidase is dependent on several endogenous and exogenous factors, such as culture age (OLSON et al 1969), temperature (DE lONG et al 1968) and growth hormone balance ( ARNISON & BOLL 1976).…”

Section: Introductionmentioning

confidence: 94%

Release of Peroxidases by Cultured Potato Cells

Bredemeijer¹,

Burg²,

Ramulu³

et al. 1985

Acta Botanica Neerlandica

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Electrophoretic analysis of peroxidase isoenzymes and other proteins present in the culture medium of potato (Solanum tuberosum L. cv. Bintje) cells in suspension culture was carried out by isoelectric focusing and starch gel electrophoresis. The results showed that during the exponential growth phase, the number of peroxidase isoenzymes as well as their activities increased. In addition, several other proteins have increased in the culture medium.The high specific activity of medium peroxidase as compared to that of cellular peroxidase and differences in the release of various peroxidase isoenzymes suggest that the peroxidase was released by specific secretion.

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Calcium-controlled peroxidase secretion by sugarbeet cell suspensions in relation to habituation

Cited by 31 publications

References 20 publications

Peroxidase in Suspension Cultures of Haplopappus Gracilis

Peroxidase in Suspension Cultures of Haplopappus Gracilis

Induction of peroxidase by Fe2+ in detached rice leaves

Release of Peroxidases by Cultured Potato Cells

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