Calcium-dependent ABA signaling functions in stomatal immunity by regulating rapid SA responses in guard cells

Ou, Xiaobin; Li, Tianqi; Zhao, Yanling; Chang, Yuankai; Wu, Lie; Chen, Guoqingzi; Day, Brad; Jiang, Kun

doi:10.1016/j.jplph.2021.153585

Cited by 14 publications

(9 citation statements)

References 57 publications

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“…With the measurement of SA and JA contents after fungal invasion, our results revealed that SA accumulated significantly and the JA level decreased significantly upon C. fulvum invasion, indicating the existence of crosstalk between SA and JA signaling pathway at the early stage of invasion. In addition, our results revealed that C. fulvum invasion also activated the ABA signaling pathway, which may be induced to trigger the ABA-dependent stomata immune response against pathogen entry ( Ou et al., 2022 ). There are also many DEGs involved in the auxin signaling pathway.…”

Section: Discussionmentioning

confidence: 89%

Physiological and transcriptome profiling revealed defense networks during Cladosporium fulvum and tomato interaction at the early stage

Peng

Sun

et al. 2022

Front. Plant Sci.

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Tomato leaf mold caused by Cladosporium fulvum (C. fulvum) is a serious fungal disease which results in huge yield losses in tomato cultivation worldwide. In our study, we discovered that ROS (reactive oxygen species) burst was triggered by C. fulvum treatment in tomato leaves. RNA-sequencing was used to identify differentially expressed genes (DEGs) induced by C. fulvum inoculation at the early stage of invasion in susceptible tomato plants. Gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to annotate functions of DEGs in tomato plants. Based on our comparative analysis, DEGs related to plant-pathogen interaction pathway, plant hormone signal transduction pathway and the plant phenylpropanoid pathway were further analyzed. Our results discovered that a number of core defense genes against fungal invasion were induced and plant hormone signal transduction pathways were impacted by C. fulvum inoculation. Further, our results showed that SA (salicylic acid) and ABA (abscisic acid) contents were accumulated while JA (jasmonic acid) content decreased after C. fulvum inoculation in comparison with control, and quantitative real-time PCR to detect the relative expression of genes involved in SA, ABA and JA signaling pathway further confirmed our results. Together, results will contribute to understanding the mechanisms of C. fulvum and tomato interaction in future.

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Section: Discussionmentioning

confidence: 89%

Physiological and transcriptome profiling revealed defense networks during Cladosporium fulvum and tomato interaction at the early stage

Peng

Sun

et al. 2022

Front. Plant Sci.

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“…The Osaim1 mutant had c. 90% less SA than WT in shoot and displayed increased stomatal aperture and reduced shoot temperatures relative to those in WT. NPR1 is the master regulator of SA‐mediated responses (Dong, 2004), and SA induces stomatal closure in an NPR1‐dependent manner in Arabidopsis (Ou et al ., 2022). In rice, however, both OsNPR1 and OsWRKY45 play a crucial role in SA signaling (Shimono et al ., 2007).…”

Section: Discussionmentioning

confidence: 99%

AIM1‐dependent high basal salicylic acid accumulation modulates stomatal aperture in rice

Zhao

Wang

et al. 2023

New Phytologist

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The basal levels of salicylic acid (SA) vary dramatically among plant species. In the shoot, for example, rice contains almost 100 times higher SA levels than Arabidopsis. Despite its high basal levels, neither the biosynthetic pathway nor the biological functions of SA are well understood in rice.Combining with metabolite analysis, physiological, and genetic approaches, we found that the synthesis of basal SA in rice shoot is dependent on OsAIM1, which encodes a betaoxidation enzyme in the phenylalanine ammonia-lyase (PAL) pathway.Compromised SA accumulation in the Osaim1 mutant led to a lower shoot temperature than wild-type plants. However, this shoot temperature defect resulted from increased transpiration due to elevated steady-state stomatal aperture in the mutant. Furthermore, the high basal SA level is required for sustained expression of OsWRKY45 to modulate the steadystate stomatal aperture and shoot temperature in rice.Taken together, these results provide the direct genetic evidence for the critical role of the PAL pathway in the biosynthesis of high basal level SA in rice, which plays an important role in the regulation of steady-state stomatal aperture to promote fitness under stress conditions.

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“…The Osaim1 mutant had about 90% less SA than WT in shoot and displayed increased stomatal aperture and reduced shoot temperatures relative to those in WT. NPR1 is the master regulator of SA-mediated responses (39) and SA induces stomatal closure in an NPR1-dependent manner in Arabidopsis (40). In rice, however, both OsNPR1 and OsWRKY45 play a crucial role in SA signaling (20).…”

Section: Discussionmentioning

confidence: 99%

AIM1-dependent high basal SA accumulation modulates stomatal aperture in rice

Zhao

Wang

et al. 2022

Preprint

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The basal levels of salicylic acid (SA), an important plant hormone, vary dramatically among plant species. In the shoot, for example, the monocot plant rice contains almost 100 times higher SA levels than the dicot model plant Arabidopsis. Despite its high basal levels, neither the biosynthetic pathway nor the biological functions of SA is well understood in rice. Here, we report that the synthesis of basal SA in rice shoot is not altered in the mutant for the ISOCHORISMATE SYNTHASE (ICS) gene, but drastically reduced in the mutant for OsAIM1, which encodes a beta-oxidation enzyme in the phenylalanine ammonia-lyase (PAL) pathway. Analogous to its role in thermogenesis, compromised SA accumulation in the Osaim1 mutant led to a lower shoot temperature than wild-type plants. However, this shoot temperature defect was resulted from increased transpiration due to elevated steady-state stomatal aperture in the mutant. Furthermore, the high basal shoot SA level is required for sustained expression of WRKY45 to modulate the steady-state stomatal aperture and shoot temperature in rice. Taken together, these results provide the direct genetic evidence for the critical role of the PAL pathway in the biosynthesis of high levels of basal SA, which play an important role in the regulation of steady-state stomatal aperture to promote fitness under both normal and stress conditions.

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Calcium-dependent ABA signaling functions in stomatal immunity by regulating rapid SA responses in guard cells

Cited by 14 publications

References 57 publications

Physiological and transcriptome profiling revealed defense networks during Cladosporium fulvum and tomato interaction at the early stage

Physiological and transcriptome profiling revealed defense networks during Cladosporium fulvum and tomato interaction at the early stage

AIM1‐dependent high basal salicylic acid accumulation modulates stomatal aperture in rice

AIM1-dependent high basal SA accumulation modulates stomatal aperture in rice

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