Calcium ions rescue human lung epithelial cells from the toxicity of zinc oxide nanoparticles

Hanagata, Nobutaka; Morita, Hiromi

doi:10.2131/jts.40.625

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…The maximum concentration of Zn +2 ion released by present multi‐ion doped HA samples was ~2.4 ppm and at this concentration no cytotoxicity on NIH3T3 cells can be seen from Figure . This understandable as the Zn +2 ion concentration up to 8 ppm do not induced any cell death and presence of Ca +2 in the culture media can upregulate the cell cycle function leading to significant decrease in the inhibitory effect of Zn +2 if any . Zinc ions also found to protect the mouse fibroblast cells from toxicity of other ions such as Co +2 .…”

Section: Resultsmentioning

confidence: 81%

In vitro cytotoxicity and ion release of multi‐ion doped hydroxyapatite

Reger

Kundu

Balla

et al. 2018

Int J Applied Ceramic Tech

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Multi-ion doped hydroxyapatite (HA) is gaining more attention due to its potential in enhancing multifunctional biological, structural, and mechanical properties for orthopedic and dental applications. In this study, HA doped with multiple cations (Sr +2 , Zn +2 , Ag + ) and anion (F − ) was prepared by high-energy ball milling. Sintered HA samples were evaluated for their in vitro cytocompatibility, ion release, and bioactivity. The composition of multi-ion doped HA was optimized usingDesign of experiments (DOE). Our analysis showed that the contribution of each dopant on cell proliferation changes with culture duration. During first 3 days, F − exhibited strongest influence and during 7-day proliferation Sr +2 and Ag + had maximum influence. Binary ion doping found to have strong interaction on cell proliferation, while the ternary and quaternary ion doping did not show any interactions. In general, up to twofold increase in the cell viability was achieved with ternary and quaternary ion doping consisting of Sr +2 , Zn +2 , Ag + and F − .Although large number of compositions has been identified to exhibit better in vitro cell viability than pure HA, for enhanced long-term cytocompatibility the compositions of multi-ion doped HA would be 2.5Sr-2.5Zn-2.5Ag, 2.5Sr-5Zn-2.5Ag, and 5Sr-2.5Zn-2.5Ag with up to 5 wt% F. K E Y W O R D Sbioactivity, doped HA, in vitro, ion release, multi-ion

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Section: Resultsmentioning

confidence: 81%

In vitro cytotoxicity and ion release of multi‐ion doped hydroxyapatite

Reger

Kundu

Balla

et al. 2018

Int J Applied Ceramic Tech

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“…Most of the antibacterial compounds are of bulk complex materials having high toxicological side effects [1][2][3][4][5]. Recent studies on antibacterial activity using nanoparticles (NPs) have shown to reduce the side effects, although the data are both limited and controversial [6][7][8][9]. The antibacterial activity of NPs is attributed to their nano scale dimensions, changed electronic/optical transitions and high surface to volume ratio which leads to improved surface chemistry and quantum confinement effects [10,11].…”

Section: Introductionmentioning

confidence: 99%

Cinnamomum verum Bark Extract Mediated Green Synthesis of ZnO Nanoparticles and Their Antibacterial Potentiality

et al. 2020

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Cinnamomum verum plant extract mediated propellant chemistry route was used for the green synthesis of zinc oxide nanoparticles. Prepared samples were confirmed for their nano regime using advanced characterization techniques such as powder X-ray diffraction and microscopic techniques such as scanning electron microscopy and transmission electron microscopy. The energy band gap of the green synthesized zinc oxide (ZnO)-nanoparticles (NPs) were found between 3.25–3.28 eV. Fourier transmission infrared spectroscopy shows the presence of Zn-O bond within the wave number of 500 cm−1. SEM images show the specific agglomeration of particles which was also confirmed by TEM studies. The green synthesized ZnO-NPs inhibited the growth of Escherichia coli and Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 125 µg mL−1 and 62.5 µg mL−1, respectively. The results indicate the prepared ZnO-NPs can be used as a potential antimicrobial agent against harmful pathogens.

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“…As well, when injected ZnO NPs intraperitoneally (20–30 nm, 25 mg/kg), there are no significant effects in Zn, Na+, and K+ level in the brain, whereas Fe and Ca2+ level were clearly reduced (Amara et al, ). Additionally, in a previous study using human lung epithelial cells, ZnO NPs‐induced cytotoicity was mitigated by supplementing the culture medium with Ca2+ (Hanagata and Morita, ). On the other hand, in other studies, ZnO NPs have been shown to disrupt intracellular calcium homeostasis by elevating intracellular Ca2+ level (Wang et al, ; Guo et al, ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of distribution and toxicity of different types of zinc‐based nanoparticles

Park

Jeong

Yoon

et al. 2016

Environmental Toxicology

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Zinc-based nanoparticles (Zn-NPs), mainly zinc oxide (ZnO) NPs, have promising application in a wide area, but their potential harmful effects on environment and human health have been continuously raised together with their high dissolution rate. In this study, we coated the surface of ZnO NPs with phosphate (ZnP NPs) and sulfide (ZnS NPs) which have very low solubility in water, administered orally (0.5 and 1 mg/kg) to mice for 28 days, and then compared their biodistribution and toxicity. As expected, ZnO NPs were rapidly ionized in an artificial gastric fluid. On the other hand, ZnO NPs were more particlized in an artificial intestinal fluid than ZnP and ZnS NPs. After repeated dosing, all three types of Zn-NPs the most distributed in the spleen and thymus and altered the level of redox reaction-related metal ions in the tissues. We also found that three types of Zn-NPs clearly disturb tissue ion homeostasis and influence immune regulation function. However, there were no remarkable difference in distribution and toxicity following repeated exposure of three types of Zn-NPs, although Na+ and K+ level in the spleen and thymus were notably higher in mice exposed to ZnO NPs compared to ZnP and ZnS NPs. Taken together, we suggest that all three types of Zn-NPs may influence human health by disrupting homeostasis of trace elements and ions in the tissues. In addition, the surface transformation of ZnO NPs with phosphate and sulfide may not attenuate toxicity due to the higher particlization rate of ZnO NPs in the intestine, at least in part. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1363-1374, 2017.

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Calcium ions rescue human lung epithelial cells from the toxicity of zinc oxide nanoparticles

Cited by 6 publications

References 47 publications

In vitro cytotoxicity and ion release of multi‐ion doped hydroxyapatite

In vitro cytotoxicity and ion release of multi‐ion doped hydroxyapatite

Cinnamomum verum Bark Extract Mediated Green Synthesis of ZnO Nanoparticles and Their Antibacterial Potentiality

Comparison of distribution and toxicity of different types of zinc‐based nanoparticles

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