Calcium metabolism of rats with varying degrees of insulinopenia

Locatto, M. E.; Fernández, María del Carmen; Abranzon, H.; Caferra, Digna A.; Puche, R. C.

doi:10.1016/0169-6009(90)90115-v

Cited by 11 publications

(2 citation statements)

References 40 publications

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“…The impact of insulin deficiency was greater on bone collagen than on the mineral mass. Although these rats had higher serum PTH levels than their controls, they had significantly diminished rates of bone Ca accretion and resorption, reduced areas of osteocytes lacunae and hypocalcemia after 24 h of fasting199.…”

Section: Basic Researchmentioning

confidence: 80%

An overview of Argentine contributions to diabetes research in the decade of the 1990s

Gagliardino

2000

Diabetes Metab. Res. Rev.

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Argentina has a longstanding tradition of diabetes research, beginning with the seminal work of Prof. Bernardo A. Houssay, who was awarded the first Nobel Prize in Medical Sciences for his studies on the relationship between diabetes and pituitary function. Prof. Luis F. Leloir, who was also awarded the Nobel Prize for his work in carbohydrate metabolism, also inspired younger generations of biologists to work in the field of diabetes research. The aim of this paper is to provide a review of the contributions of Argentine researchers during the 1990s. This manuscript includes only reports of Argentine researchers working on diabetes in local laboratories and quoted in Medline. Thus, important contributions not reported in journals included in Medline or produced by Argentine researchers working abroad may have been omitted. The material consists of a brief description of clinical research (epidemiology and costs, metabolic control, associated risk factors, immunological aspects, and other clinical studies) and basic research (animal model with spontaneous diabetes, islet morphology and function in normal and pathological conditions, insulin action, metabolic disorders related to diabetes, and some miscellaneous effects related to drug‐induced diabetes). Altogether, a broad idea of the continuous contribution of our national research to the international field of diabetes is provided, as well as a list of Argentine researchers and research centers devoted to the study of diabetes. Copyright © 2000 John Wiley & Sons, Ltd.

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Section: Basic Researchmentioning

confidence: 80%

An overview of Argentine contributions to diabetes research in the decade of the 1990s

Gagliardino

2000

Diabetes Metab. Res. Rev.

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“…The loss of BMC is aggravated in poorly controlled diabetes by renal calcium leak (Gregorio et al 1994). Osteoporosis is present in experimental diabetes of rats (Verhaeghe et al 1990, Jara et al 1995 but the involvement of PTH is controversial, with some (Hough et al 1981, 1982, Shires et al 1981 but not all (Glajchen et al 1988, Locatto et al 1990, Jara et al 1995, Hamilton et al 2000 studies reporting reduced circulating PTH concentrations. Bone mineralisation is also regulated by prostaglandins (Conaway et al 1986) which potentially could be a pathway for intervention in diabetes, and a target for the development of treatments.…”

Section: Introductionmentioning

confidence: 99%

gamma-Linoleic acid and ascorbic acid ameliorate the effects of experimental diabetes on electrolyte and bone homeostasis in pregnant rats

Braddock¹,

Simán²,

Hamilton³

et al. 2002

Journal of Endocrinology

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Experimental diabetes in rats is associated with excessive electrolyte loss in the urine, which is further accentuated by pregnancy, particularly of Ca. Supplementation with essential fatty acids and antioxidants has proven beneficial in treating several types of complications, including nephropathy. The present study investigated the effect of -linoleic acid (GLA; 500 mg/kg per day; group DG) and ascorbate (290 mg/kg per day; group DA), alone and in combination (group DGA), as well as ascorbyl-GLA (790 mg/kg per day; group DASG), on urinary electrolyte output and skeletal composition in pregnant streptozotocin-diabetic rats. Urine was collected in metabolism cages before and throughout pregnancy. Diabetic rats (DP) increased their urine volume as compared with control (CP) throughout the experiment, reaching an output of more than 13 times that of the control group by the end of pregnancy (CP 24 4, DP 316 21, DG 223 21, DA 221 14, DASG 163 17, DGA 220 19 ml urine/24 h). Concomitant with increased urine volume was a reduction of urinary Na (CP 47 14, DP 22 5 mmol/l), K (CP 210 34, DP 31 1 mmol/l) and Mg (CP 14 1, DP 3·8 0·2 mmol/l) concentration, but not of Ca concentration (CP 5·4 1·5, DP 6·3 0·6 mmol/l), and hence total Ca loss was relatively most severe. All the treatments reduced urine volume with no effects on electrolyte concentration as compared with DP, with no significant difference between the treatments. A reduced bone size and bone Ca content was partially ameliorated by the diet supplementation. We have concluded that GLA and ascorbate, alone or in combination, prevent urinary electrolyte loss in pregnant rats and do so by reducing urine production.

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Low Bone Density with Normal Bone Turnover in Ovariectomized and Streptozotocin-Induced Diabetic Rats

et al. 1998

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Diabetes and estrogen deficit are known causes of osteopenia, diabetes being associated with a low bone turnover and estrogen deficit with a high bone turnover. In the present work, we studied the effect of combined ovariectomy and diabetes on bone mineral content (BMC) and bone mineral density (BMD) and several bone markers in the rat. Four groups of rats were studied: control (C), ovariectomized (O), diabetic (D), and ovariectomized and diabetic (DO). Twelve weeks after starting the experiments, BMC and BMD of the first six lumbar vertebrae were measured; a bone formation marker (BGP) and a bone resorption marker (free collagen cross-links, PYD) were also analyzed. Diabetic rats showed diminished gain in bone mass, BMC (D: 0.417 +/- 0.028 g, DO: 0.422 +/- 0.020 g) and BMDs (D: 0.171 +/- 0.006 g/cm2, DO: 0.174 +/- 0.006 g/cm2) both being significantly (P < 0.001) lower than those of control (C: BMC 0.727 +/- 0.024 g and BMD 0.258 +/- 0.004 g/cm2) and ovariectomized (O: BMC 0.640 +/- 0.044 g and BMD 0.240 +/- 0.009 g/cm2) groups. Moreover, the BMC and BMD of the C group were significantly (P < 0.05) higher than that of the O group. BGP and PYD levels were significantly (P < 0.01) higher in the O group (BGP: 138.2 +/- 16.8 ng/ml, PYD: 270.2 +/- 17.8 nM/mM) than those found in the control rats (BGP: 44.7 +/- 4.8 ng/ml, PYD: 165.6 +/- 12.5 nM/mM); the D group showed significantly (P < 0.01) lower values (BGP: 27.4 +/- 14.6 ng/ml, PYD: 55.0 +/- 7.4 nM/mM) than those of the control group. The DO group showed similar levels (BGP: 43.4 +/- 5.1 ng/ml, PYD: 146.7 +/- 14.6 nM/mM) to those found in the C group. Although bone marker levels in the O and D groups were in accordance with those expected in these situations, in the DO group the corresponding levels are apparently "normal." Also, the decrease of gain in bone mass observed after combining estrogen deficit and diabetes (DO group) did not seem to be more marked than that caused by diabetes alone.

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Calcium metabolism of rats with varying degrees of insulinopenia

Cited by 11 publications

References 40 publications

An overview of Argentine contributions to diabetes research in the decade of the 1990s

An overview of Argentine contributions to diabetes research in the decade of the 1990s

gamma-Linoleic acid and ascorbic acid ameliorate the effects of experimental diabetes on electrolyte and bone homeostasis in pregnant rats

Low Bone Density with Normal Bone Turnover in Ovariectomized and Streptozotocin-Induced Diabetic Rats

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