Calcium mobilizations in response to changes in the gravity vector in<i>Arabidopsis</i>seedlings

Tatsumi, Hitoshi; Toyota, Masatsugu; Furuichi, Teiichi; Sokabe, Masahiro

doi:10.4161/psb.29099

Cited by 21 publications

(17 citation statements)

References 54 publications

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“…Perhaps, in time, responses will be made evident at the cellular or intracellular levels through changes in their respective biophysical properties (Tatsumi et al . ).…”

Section: Discussionmentioning

confidence: 97%

The effect of lunisolar tidal acceleration on stem elongation growth, nutations and leaf movements in peppermint (Mentha × piperita L.)

Zajączkowska

Barlow

2017

Plant Biol J

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Orbital movement of the Moon generates a system of gravitational fields that periodically alter the gravitational force on Earth. This lunar tidal acceleration (Etide) is known to act as an external environmental factor affecting many growth and developmental phenomena in plants. Our study focused on the lunar tidal influence on stem elongation growth, nutations and leaf movements of peppermint. Plants were continuously recorded with time-lapse photography under constant illumination as well in constant illumination following 5 days of alternating dark-light cycles. Time courses of shoot movements were correlated with contemporaneous time courses of the Etide estimates. Optical microscopy and SEM were used in anatomical studies. All plant shoot movements were synchronised with changes in the lunisolar acceleration. Using a periodogram, wavelet analysis and local correlation index, a convergence was found between the rhythms of lunisolar acceleration and the rhythms of shoot growth. Also observed were cyclical changes in the direction of rotation of stem apices when gravitational dynamics were at their greatest. After contrasting dark-light cycle experiments, nutational rhythms converged to an identical phase relationship with the Etide and almost immediately their renewed movements commenced. Amplitudes of leaf movements decreased during leaf growth up to the stage when the leaf was fully developed; the periodicity of leaf movements correlated with the Etide rhythms. For the fist time, it was documented that lunisolar acceleration is an independent rhythmic environmental signal capable of influencing the dynamics of plant stem elongation. This phenomenon is synchronised with the known effects of Etide on nutations and leaf movements.

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“…Perhaps, in time, responses will be made evident at the cellular or intracellular levels through changes in their respective biophysical properties (Tatsumi et al . ).…”

Section: Discussionmentioning

confidence: 97%

The effect of lunisolar tidal acceleration on stem elongation growth, nutations and leaf movements in peppermint (Mentha × piperita L.)

Zajączkowska

Barlow

2017

Plant Biol J

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“…The first model suggests a role for mechanosensitive ion channels in gravity sensing. However, none of the plant mechanosensitive ion channels identified so far have been shown to contribute to gravitropism [24,25]. On the other hand, experiments involving the transgenic AEQUORIN Ca 2+ -reporter system demonstrated the existence of biphasic spikes in cytosolic Ca 2+ within seconds of gravity sensing [26,27].…”

Section: Gravity Sensing In the Statocytes Involves Unknown Receptorsmentioning

confidence: 99%

Molecular Mechanisms of Root Gravitropism

et al. 2017

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Plant shoots typically grow against the gravity vector to access light, whereas roots grow downward into the soil to take up water and nutrients. These gravitropic responses can be altered by developmental and environmental cues. In this review, we discuss the molecular mechanisms that govern the gravitropism of angiosperm roots, where a physical separation between sites for gravity sensing and curvature response has facilitated discovery. Gravity sensing takes place in the columella cells of the root cap, where sedimentation of starch-filled plastids (amyloplasts) triggers a pathway that results in a relocalization to the lower side of the cell of PIN proteins, which facilitate efflux of the plant hormone auxin efflux. Consequently, auxin accumulates in the lower half of the root, triggering bending of the root tip at the elongation zone. We review our understanding of the molecular mechanisms that control this process in primary roots, and discuss recent insights into the regulation of oblique growth in lateral roots and its impact on root-system architecture and soil exploration.

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“…To our knowledge, mainly positive stimulus-dependent Ca 2+ responses are reported in the literature, even for centrifugation experiments (Toyota et al 2007;Tatsumi et al 2014). In the latter studies, however, whole seedlings were centrifuged for up to 300 g for extended periods.…”

Section: Discussionmentioning

confidence: 97%

Intracellular Calcium Decreases Upon Hyper Gravity-Treatment of Arabidopsis Thaliana Cell Cultures

Neef

Denn

Ecke

et al. 2015

Microgravity Sci. Technol.

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Cell cultures of Arabidopsis thaliana (A.t.) respond to changes in the gravitational field strength with fluctuations of the amount of cytosolic calcium (Ca 2+ ). In parabolic flight experiments, where hyper-and μg phases follow each other, μg clearly increased Ca 2+ , while hyperg caused a slight reduction. Since the latter observation had not been reported before, we studied this effect in more detail. Using a special centrifuge for heavy items (ZARM, Bremen, Germany), we determined the hyper-gdependent intracellular Ca 2+ level with transgenic cell lines expressing the Ca 2+ sensor, cameleon. This sensor exhibits a shift in fluorescence from 480 to 530 nm in response to Ca 2+ binding. The data show a drop in the intracellular Ca 2+ concentration with a threshold gravity of around 3 g. This is above hypergravity levels achieved during parabolic flights (1.8 g). The use of mutants with different sub-cellular targets of cameleon expression (nucleus, tonoplast, plasma membrane) gave the same results, i.e. Ca 2+ is obviously exported from several intracellular compartments.

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Calcium mobilizations in response to changes in the gravity vector inArabidopsisseedlings

Cited by 21 publications

References 54 publications

The effect of lunisolar tidal acceleration on stem elongation growth, nutations and leaf movements in peppermint (Mentha × piperita L.)

The effect of lunisolar tidal acceleration on stem elongation growth, nutations and leaf movements in peppermint (Mentha × piperita L.)

Molecular Mechanisms of Root Gravitropism

Intracellular Calcium Decreases Upon Hyper Gravity-Treatment of Arabidopsis Thaliana Cell Cultures

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