Calcium transport in and out of brain nerve endings in vitro—the role of synaptosomal plasma membrane Ca2+-ATPase in Ca2+-extrusion

Lin, Shih-Chia C.; Way, E. Leong

doi:10.1016/0006-8993(84)91422-7

Cited by 13 publications

(3 citation statements)

References 38 publications

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“…The growth cone currents described here might participate in this process in two ways: by supplying Ca2+ needed for contraction, and by aligning actin and myosin fibers under the influence of an axial voltage gradient set up by the currents. Other possible effects of calcium currents include effects on tubulin polymerization and microtubule formation [Tsui et al, 19831 and protein phosphorylation [Lin and Way, 1984;Zurgil and Zisapel, 19841. Axial lntracellular Voltage Gradients Generated by Steady Currents, and the…”

Section: Possible Cellular Role Of Growth Cone Currentsmentioning

confidence: 99%

Steady growth cone currents revealed by a novel circularly vibrating probe: A possible mechanism underlying neurite growth

Freeman

Manis

Snipes

et al. 1985

J of Neuroscience Research

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The rate and direction of neurite growth have been shown in a number of studies to be determined by the distribution of adhesive sites on the growth cone. Recent evidence showing that the application of extrinsic electric fields can redistribute membrane molecules and alter both the rate and direction of neurite growth have raised the question whether endogenous electric fields might be produced by steady currents in growth cones. To investigate this question, we have devised a novel circularly vibrating microprobe capable of measuring current densities in the range of 5 nA/cm2 (near the theorectical limit of sensitivity), with a spatial resolution of 2 micron. The design of this device and the development of a novel algorithm for computing current vectors on-line is described. Using this probe we have found that cultured goldfish retinal ganglion cell growth cones generate steady inward currents at their tips. The measured currents, in the range of 10-100 nA/cm2, appear to flow into the filopodia at their tips and back outward near the junctures of the filopodia and the growth cone. The currents appear to be produced only during active growth. Ion substitution experiments support the conclusion that the majority of this current is carried by Ca2+ ions, which we postulate flow through a population of activated voltage-sensitive Ca2+ channels located on the filopodial tips. Calculation of the transmembrane current density (4 X 10(-6) nA/cm2) leads to an estimate of channel density (10 channels/micron2) in close agreement with the measured density of Ca2+ channels in other systems. The assumption that calcium channel proteins are conveyed to nerve terminals by active transport, whereas sodium channel proteins are conveyed passively by a slower somatofugal diffusion process [Strichartz et al, 1984], would explain why developing neurons tend to display Ca2+-sensitive electrogenesis at their growing tips, and Na+-sensitive action potentials later in development. In order to gain some insight into the possible role of these steady growth currents, we estimated the membrane depolarization and axial voltage gradient they produce. It is likely that the currents produce sufficient membrane depolarization (approximately equal to 4 mV) to cause autogenous activation of ion channel permeabilities. Similarly, the axial voltage gradient (approximately equal to 4 mV/cm) would be expected to move intracytoplasmic vesicles by electrophoresis at a rate (20-40 microns/hr) very close to that at which the filopodia are observed to grow.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Possible Cellular Role Of Growth Cone Currentsmentioning

confidence: 99%

Steady growth cone currents revealed by a novel circularly vibrating probe: A possible mechanism underlying neurite growth

Freeman

Manis

Snipes

et al. 1985

J of Neuroscience Research

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“…The plasma membrane of excitable cells possesses two systems for transporting calcium out of the cell towards the calcium concentration gradienta Na+/Ca2+-exchange system and a calcium translocating ATPase (Lin & Way 1984;Snelling & Nicholls 1985). Incubation of isolated rat brain synaptosomes with anaesthetics or with ethanol, leads to a decrease in the (Ca'+/Mg+)-ATPase activity and an increase in fluidity (Harris 1983;Garrett & Ross 1983;Leslie et al 1983;Harris & Bruno 1985).…”

mentioning

confidence: 99%

The Effect of Toluene Exposure for up to 18 Months (78 Weeks) on the (Ca²⁺/Mg²⁺)ATPase and Fluidity of Synaptosomal Membranes Isolated from Rat Brain

Edelfors

Ravn-Jonsen

1989

Pharmacology & Toxicology

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The effect of toluene on the central nerve system was studied by using rat brain synaptosomal membranes as in vitro and in vivo models. The activity of Ca2+/Mg2+ ATPase and the membrane fluidity were determined. Short-term exposure in vivo to 500 p.p.m. of toluene had an inhibitory effect on the enzyme studied whereas long-term exposure to toluene caused an increased activity. Exposure to toluene had no effect at all on the membrane fluidity. The in vitro experiment showed an effect of toluene on both parameters. The alteration in the enzyme activity and membrane fluidity was parallel in the exposed animals as well as those of control. Our results show that long-term exposure to toluene affects nerve cell membranes by other mechanisms than those observed under in vitro conditions.

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“…We also identified the male-enhanced antigen 1 (mea1) in the brain of medaka. Notably, the mea1 protein was reported to be expressed specifically in elongated spermatids and residual bodies, which suggests a functional role in Pan et al, 2014;Hussain and Macklin, 2017 Tight junction signaling MYH6, MYL2, MYL4, PPP2R3A Luissint et al, 2012;Tietz and Engelhardt, 2015 Calcium signaling MYH6, MYL2, MYL4, TNNT3 Heizmann, 1993;Kawamoto et al, 2012;Bading, 2013 RhoA signaling MYL2, MYL4, TTN Govek et al, 2005;Gross et al, 2007;Stankiewicz and Linseman, 2014 CXCR4 signaling MYL2, MYL4, PRKD1 Jiang et al, 2013;Cheng et al, 2017;Bonham et al, 2018 Thrombin signaling MYL2, MYL4, PRKD1 Wang and Reiser, 2003;Ben Shimon et al, 2015;Krenzlin et al, 2016 mTOR signaling PPP2R3A, PRKD1, RPS27 Graber et al, 2013;Lee, 2015;Ryskalin et al, 2017 Calcium transport I ANXA5 Lin and Way, 1984;Heizmann, 1993;Bading, 2013 Protein kinase A signaling MYL2, MYL4, PRKD1, TTN Gervasi et al, 2007;Huang et al, 2011;Dagda and Das Banerjee, 2015 PAK signaling MYL2, MYL4 Kreis and Barnier, 2009; Civiero and Greggio, 2018 Castillo et al, 2012;Augustin and Lovinger, 2018 Endoplasmic reticulum stress pathway CASP3 Raghubir et al, 2011;Hashimoto and Saido, 2018;Tan et al, 2018 spermiogenesis (Ohinata et al, 2002). Spindlin, a maternal effect factor associated with the meiotic spindle during the meiosis of oocytes (Zhang et al, 2015), is responsible for the oocyte-to-embryo transition in carp fish (Sun et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Proteomic Response of the Brain to Hypoxic Stress in Marine Medaka Fish (Oryzias melastigma)

et al. 2021

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Hypoxia is a serious issue that affects the marine environment, with a growing number of hypoxic “dead zones” occurring each year. Reports have indicated that hypoxia is detrimental to the reproductive function and sexual development of fish via the disruption of endocrine signaling in organs involved in the hypothalamus–pituitary–gonad axis, including the brain. While we previously reported that hypoxia induces transcriptome-wide alterations in the brain of marine medaka (Oryzias melastigma), whether these effects were reflected at the protein level remains unclear. Therefore, the present study used high-throughput proteomic sequencing along with bioinformatics analysis to assess the short-term and multi-generational effects of hypoxia on the brain proteome of O. melastigma. We identified 36,567 peptides and 7,599 proteins (1% false discovery rate in brain samples), with functions involved in cellular and metabolic processes such as signaling and reproductive processes as well as energy production and conversion. Furthermore, we determined that hypoxia resulted in the significant differential expressions of 33 upregulated and 69 downregulated proteins in the short-term exposure group and 24 upregulated and 52 downregulated proteins in the multi-generational exposure group. Pathway enrichment analysis of the deregulated proteins indicated that hypoxia could impair brain function by altering arachidonic acid metabolism, tight junctions, and adrenergic signaling under short-term hypoxic exposure and by altering p53 and PI3K–Akt signaling under multi-generational hypoxic exposure, which may lead to the onset of neurodegenerative disorders including Alzheimer’s disease and amyotrophic lateral sclerosis. Ingenuity pathway analysis of the deregulated proteins showed that hypoxia affected common signaling pathways in the brain (e.g., integrin, paxillin, and epithelial adherens junction signaling) under both short-term and multi-generational exposures. Hypoxia also deregulated pathways specific to short-term exposure (including integrin-linked kinase, calcium, and integrin signaling) and multi-generational exposure (including sphingosine-1-phosphate signaling, endocannabinoid neuronal synapse pathway, and endoplasmic reticulum stress pathway). Overall, our results provide additional insights into the mechanisms of hypoxia disrupting neuronal function at the protein level in marine medaka.

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Calcium transport in and out of brain nerve endings in vitro—the role of synaptosomal plasma membrane Ca2+-ATPase in Ca2+-extrusion

Cited by 13 publications

References 38 publications

Steady growth cone currents revealed by a novel circularly vibrating probe: A possible mechanism underlying neurite growth

Steady growth cone currents revealed by a novel circularly vibrating probe: A possible mechanism underlying neurite growth

The Effect of Toluene Exposure for up to 18 Months (78 Weeks) on the (Ca²⁺/Mg²⁺)ATPase and Fluidity of Synaptosomal Membranes Isolated from Rat Brain

Proteomic Response of the Brain to Hypoxic Stress in Marine Medaka Fish (Oryzias melastigma)

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Calcium transport in and out of brain nerve endings in vitro—the role of synaptosomal plasma membrane Ca2+-ATPase in Ca2+-extrusion

Cited by 13 publications

References 38 publications

Steady growth cone currents revealed by a novel circularly vibrating probe: A possible mechanism underlying neurite growth

Steady growth cone currents revealed by a novel circularly vibrating probe: A possible mechanism underlying neurite growth

The Effect of Toluene Exposure for up to 18 Months (78 Weeks) on the (Ca2+/Mg2+)ATPase and Fluidity of Synaptosomal Membranes Isolated from Rat Brain

Proteomic Response of the Brain to Hypoxic Stress in Marine Medaka Fish (Oryzias melastigma)

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The Effect of Toluene Exposure for up to 18 Months (78 Weeks) on the (Ca²⁺/Mg²⁺)ATPase and Fluidity of Synaptosomal Membranes Isolated from Rat Brain