1977
DOI: 10.1094/phyto-67-808
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Calcofluor: An Optical Brightener for Fluorescence Microscopy of Fungal Plant Parasites in Leaves

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Cited by 130 publications
(65 citation statements)
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“…Three replications were carried out. Leaf segments were prepared for fluorescence microscopy (ROHRINGER et al 1977), but instead of Calcofluor we used Uvitex 2B (Ciba-Geigy). The preparations were examined at 1OOx with a Leica epifluorescence microscope (DM LB, 330 to 380 nm wave length transmission).…”
Section: Microscopic Observationsmentioning
confidence: 99%
“…Three replications were carried out. Leaf segments were prepared for fluorescence microscopy (ROHRINGER et al 1977), but instead of Calcofluor we used Uvitex 2B (Ciba-Geigy). The preparations were examined at 1OOx with a Leica epifluorescence microscope (DM LB, 330 to 380 nm wave length transmission).…”
Section: Microscopic Observationsmentioning
confidence: 99%
“…and 10 leaf sections 10 d.p.i. These were prepared, using Uvitex 2B (Ciba-Geigy Corp., Ardsley, NY, USA) (Niks & Dekens, 1991), as whole mounts for fluorescence microscopy (Rohringer et al, 1977;Kuck et al, 1981). Observations of infection sites on leaf segments were carried out at ×100 or ×400 magnification with a Nikon Optiphot epifluorescence microscope, using the filter combinations UV-1A for fungal structures and B-2A for autofluorescence measurements.…”
Section: Microscopic Components Of Resistancementioning
confidence: 99%
“…Light microscopy Leaf pieces of approximately 2 cm length were cleared and stained according to Rohringer et al [30], except that Uvitex 2B (Ciba-Geigy) rather than calcofluor was used . The fungal infection structures were observed using fluorescence microscopy with a 365 nm excitation filter (incident light) and a 420 nm barrier filter .…”
Section: Plant Inoculation and Treatmentsmentioning
confidence: 99%