Calculation of gestational age in late second and third trimesters by ex vivo magnetic resonance spectroscopy of amniotic fluid

Cohn, Brad; Fukuchi, Emily; Joe, Bonnie N.; Swanson, Mark G.; Kurhanewicz, John; Yu, Jingwei; Caughey, Aaron B.

doi:10.1016/j.ajog.2010.01.046

Cited by 16 publications

(21 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One proteomic study specifically examining the effect of gestational age on AF failed to yield any significant information on the third trimester (19). A metabolomic study on second and third trimester AF samples identified metabolites associated with increasing gestational age (20). Neither of these studies attempted to provide any detailed biological interpretation of the differentially-expressed proteins in third compared with second trimester.…”

Section: Discussionmentioning

confidence: 99%

Global Gene Expression Analysis of Term Amniotic Fluid Cell-Free Fetal RNA

Hui

Wick

Edlow

et al. 2013

Obstetrics &Amp; Gynecology

View full text Add to dashboard Cite

Objective To identify the tissue expression patterns and biological pathways enriched in term amniotic fluid (AF) cell-free fetal RNA by comparing functional genomic analyses of term and second trimester AF supernatants. Methods This was a prospective whole genome microarray study comparing eight AF samples collected from eight women at term who underwent prelabor cesarean delivery and eight second trimester AF samples from routine amniocenteses. A functional annotation tool was used to compare tissue expression patterns in term and second trimester samples. Pathways analysis software identified physiological systems, molecular and cellular functions and upstream regulators that were significantly overrepresented in term AF. Results There were 2,871 significantly differentially regulated genes. In term AF, tissue expression analysis showed enrichment of salivary gland, tracheal, and renal transcripts, as compared with brain and embryonic neural cells in second trimester. Functional analysis of genes upregulated at term revealed pathways that were highly specific for postnatal adaptation, such as immune function, digestion, respiration, carbohydrate metabolism and adipogenesis. Inflammation and prostaglandin synthesis, two key processes involved in normal labor, were also activated in term AF. Conclusions Transcriptomic analysis of AF cell-free fetal RNA detects fetal maturation processes activated in term pregnancy. These findings further develop the concept of AF supernatant as a real-time gene expression “summary fluid” and support its potential for future studies of fetal development.

show abstract

Section: Discussionmentioning

confidence: 99%

Global Gene Expression Analysis of Term Amniotic Fluid Cell-Free Fetal RNA

Hui

Wick

Edlow

et al. 2013

Obstetrics &Amp; Gynecology

View full text Add to dashboard Cite

show abstract

“…In recent years, the analysis of biological fluids or tissues has been used successfully in identification of typical compounds of various physiologic or pathologic conditions by providing the underlying metabolic changes during the pregnancy period, and a steady inflow of research development has been reported in this context by using maternal blood and urine, amniotic fluid, and umbilical cord blood to characterized the relevant prenatal diseases such as preeclampsia (Austdal et al, 2014;Luque et al, 2014;Redman and Sargent, 2005), fetal malformations (Diaz et al, 2011), gestational diabetes mellitus (Bo et al, 2015;Hernandez et al, 2014), poor pregnancy outcome (Halscott et al, 2014;Hourrier et al, 2010), preterm delivery (Alexandre- Gouabau et al, 2013) and small for the gestational age (Cohn et al, 2010). Moreover, protein and nitrogen metabolism were found to play extremely important roles in pregnancy and the development of the fetus (Altobelli et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, the dynamic metabolic adaptations of maternal urine from healthy pregnancies has been evaluated by NMR-based metabolomics techniques, in which the levels of 21 metabolites including choline, creatinine, 4-deoxyerythronic acid, 4-deoxythreonic acid, furoylglycine, guanidoacetate, 3-hydroxybutyrate, and lactate were found to change significantly throughout pregnancy (Diaz et al, 2013). In addition, some targeted studies of amniotic fluid have shown that the levels of alanine, glutamine, valine, creatinine, glucose, and succinic acid reflect gestational age (Athanasiadis et al, 2011;Cohn et al, 2010;Ottolenghi et al, 2010). The advances in these previous studies consistently demonstrated the potential of metabolomics to reveal prenatal disorders and corresponding individual responses, thus setting the reference for the detection of deviant trajectories in biofluids in the context of the metabolic follow-up of pregnancy.…”

Section: Introductionmentioning

confidence: 99%

1H NMR-based metabolomics study on the physiological variations during the rat pregnancy process

Shen

Zhang

et al. 2016

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

a b s t r a c tIn this study, NMR-based metabolomics in combination with multivariate pattern recognition technologies were employed to evaluate the physiological variations in the Wistar rats' plasma that are induced by pregnancy on the gestational days (GDs) 11, 14, 17 and 20. Untargeted metabolomics analysis revealed some possible mechanism of physiological effects for healthy pregnancies and showed a metabolic trajectory during pregnancy process. The levels of 24 metabolites were found to change significantly throughout pregnancy in maternal plasma. These metabolite changes involved in varied kinds of metabolic pathways including synthesis of biological substances, microbial metabolism in diverse environments, protein digestion and absorption, carbohydrate metabolism, digestion and absorption, mineral absorption, and ATP (Adenosine Triphosphate)-binding cassette transporters (ABC transporters). The substantial cores of all the metabolic pathways are promoting fetal growth and development and regulating maternal physiological state. This work showed relevant metabolic pathways perturbation in the maternal plasma due to normal pregnancy and provided the physical basis of time-dependent metabolic trajectory against which disease-related maternal physiological responses may be better understood in future studies.

show abstract

“…HRMAS MRS metabolic profiles of AF were generated for second and third gestation trimesters . Using a targeted approach and measuring metabolite concentration through the electronic reference to access in vivo concentrations (ERETIC) method, HRMAS MRS results presented increases in creatinine and decreases in Glc, Cit, pyruvate, Cr, Ala, Glu, Lys, and Val with advancing GA ( p < 0.001).…”

Section: Applications Of Hrmas Mrs On Studies Of Other Non‐malignant mentioning

confidence: 99%

Applications of high‐resolution magic angle spinning MRS in biomedical studies II—Human diseases

et al. 2017

View full text Add to dashboard Cite

High-resolution magic angle spinning (HRMAS) magnetic resonance spectroscopy (MRS) is a powerful method for gaining insight into the physiological and pathological processes of cellular metabolism. Given its ability to obtain high resolution spectra of non-liquid biological samples, while preserving tissue architecture for subsequent histopathological analysis, the technique has become invaluable for biochemical and biomedical studies. Using HRMAS MRS, alterations in measured metabolites, metabolic ratios, and metabolomic profiles present the possibility to improve identification and prognostication of various diseases and decipher the metabolomic impact of drug therapies. In this review, we evaluate HRMAS MRS results on human tissue specimens from malignancies and non-localized diseases reported in the literature since the inception of the technique in 1996. We present the diverse applications of the technique in understanding pathological processes of different anatomical origins, correlations with in vivo imaging, effectiveness of therapies, and progress in the HRMAS methodology.

show abstract

Calculation of gestational age in late second and third trimesters by ex vivo magnetic resonance spectroscopy of amniotic fluid

Cited by 16 publications

References 34 publications

Global Gene Expression Analysis of Term Amniotic Fluid Cell-Free Fetal RNA

Global Gene Expression Analysis of Term Amniotic Fluid Cell-Free Fetal RNA

1H NMR-based metabolomics study on the physiological variations during the rat pregnancy process

Applications of high‐resolution magic angle spinning MRS in biomedical studies II—Human diseases

Contact Info

Product

Resources

About