Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus

Horák, Daniel; Plichta, Zdeněk; Starykovych, M. O.; Myronovskij, Severyn; Kit, Yu. Ya.; Chopyak, Valentyna; Stoika, Rostyslav

doi:10.1039/c5ra09280a

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…The starting nonmagnetic, as well as mgt.PGMA-NH 2 , mgt.PGMA-D1, mgt.PGMA-D2, and mgt.PGMA-D3 microspheres, had similar morphology with size increasing from 4.5 to 4.8 μm due to chemical modification (Figure a–d,f and Table ). The low value of the polydispersity index (PDI < 1.05) documented uniformity of the particle size, which is very important for maintaining unique controlled and reproducible physicochemical and biochemical properties. , Iron oxide was embedded inside the porous structure of the microspheres and close to their surface (Figure e), providing them with superparamagnetic properties …”

Section: Resultsmentioning

confidence: 99%

Antifouling Peptide Dendrimer Surface of Monodisperse Magnetic Poly(glycidyl methacrylate) Microspheres

et al. 2017

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Antifouling properties and stability in tissue fluids are crucial for the successful application of micro-and nanoparticles in biomedicine. In this study, we prepared monodisperse magnetic poly(glycidyl methacrylate) microspheres with amino groups (mgt.PGMA-NH 2 ) by a multistep swelling polymerization of glycidyl methacrylate (GMA). This was followed by ammonolysis of oxirane groups and precipitation of iron oxides inside the particle pores to make the microspheres magnetic. To suppress nonspecific protein adsorption from biological media, the microspheres were covered by three generations of a compact amino acid dendritic network (Ser-Lys-Ser/Lys-Ser/Lys-Ser) using peptide chemistry. The resulting particles did not aggregate under physiological conditions and contained ∼1 mmol of NH 2 /g that was available for further modifications. Alkyne groups accessible for click chemistry were introduced to the dendrimer-coated particles by a reaction with 4-pentynoic acid. The external particle surface and internal bulk were characterized by scanning (SEM) and transmission electron microscopy (TEM), atomic absorption (AAS), FTIR, X-ray photoelectron spectroscopy (XPS), and elemental analysis. Antifouling properties of the dendrimer and linear Ser-Ala-Ser/Ala-Ser/Ala-Ser peptide-modified mgt.PGMA-NH 2 microspheres were challenged with solutions of proteins, such as bovine serum albumin (BSA), γ-globulin (γ-Gl), fibrinogen (Fg), and a mixture of them. Finally, a model azide−alkyne cycloaddition reaction with 125 I-radiolabeled azidopentanoyl-GGGRGDSGGGY( 125 I)-NH 2 ( 125 I-N 3 -RGDS) peptide demonstrated that the dendrimer-modified particles are suitable for potential applications, including the separation of peptides and other biomolecules, diagnostics, mimetics, vaccine synthesis, etc.

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Section: Resultsmentioning

confidence: 99%

Antifouling Peptide Dendrimer Surface of Monodisperse Magnetic Poly(glycidyl methacrylate) Microspheres

et al. 2017

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“…British pharmaceutical giant GSK recently (April 2019) announced that the US Food and Drug Administration (US FDA) has approved the lupus drug benlysta for children aged 5 years and above with systemic lupus erythematosus (SLE) through priority review. SLE is the most common type of lupus, which will lead to serious long-term organ damage . In this contribution, we tried to load the new drug benlysta into sodium alginate hydrogels and systematically evaluated the effect of benlysta-loaded hydrogels on the growth of macrophages (inflammation evaluation), epidermal cells, fibroblasts, endothelial cells, etc.…”

Section: Introductionmentioning

confidence: 99%

Benlysta-Loaded Sodium Alginate Hydrogel and Its Selective Functions in Promoting Skin Cell Growth and Inhibiting Inflammation

et al. 2020

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Benlysta is a new drug approved by the US Food and Drug Administration (US FDA) in 2019 for the treatment of systemic lupus erythematosus. In this study, we loaded the benlysta in the traditional sodium alginate (SA) hydrogel to investigate the potential application of the drug-loaded hydrogel for skin dressing or hypodermic drug. Live/dead staining images and the CCK-8 results showed that the benlysta-loaded hydrogel could promote the growth of human epidermal cells (HaCat), fibroblasts (L929), and endothelial cells while inhibiting the aggregation of inflammatory cells (macrophages). In addition, the hydrogel degradation and drug release are slow and controllable, and the gel time of drug-loaded hydrogel can be adjusted by adding sodium alginate ratios according to the requirement. In summary, we prepared a time-dependent drug-loaded hydrogel for potential application in the treatment of skin injury that may be caused by other diseases.

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“…Enrichment of specific proteins in biological samples helps identify disease specific biomarkers at early stages [ 24 , 25 ]. Our previous studies were focused on identification of protein markers in the blood serum of patients suffering from multiple sclerosis (MS), rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) [ 26 , 27 ]. The original precipitation/extraction method and MALDI TOF/TOF mass spectrometry was therefore developed for isolation of the human unconventional myosin IC isoform b (p46/Myo1C) fragment ( M r ∼46 kDa) as a potential marker of the listed autoimmune diseases [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, target antigens are often contaminated by other proteins and a lot of effort, not always successful, is required to separate them. To solve these problems, magnetic microspheres containing specific protein antigens are needed to allow easy isolation of the monospecific antibodies [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Magnetic poly(2-hydroxyethyl methacrylate) microspheres for affinity purification of monospecific anti-p46 kDa/Myo1C antibodies for early diagnosis of multiple sclerosis patients

et al. 2017

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The aim of the present study is to develop new magnetic polymer microspheres with functional groups available for easy protein and antibody binding. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA-COOH) microspheres ~4 µm in diameter and containing ∼1 mmol COOH/g were synthesized by multistep swelling polymerization of 2-hydroxyethyl methacrylate (HEMA), ethylene dimethacrylate (EDMA), and 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA), which was followed by MCMEMA hydrolysis. The microspheres were rendered magnetic by precipitation of iron oxide inside the pores, which made them easily separable in a magnetic field. Properties of the resulting magnetic poly(2-hydroxyethyl methacrylate) (mgt.PHEMA) particles with COOH functionality were examined by scanning and transmission electron microscopy (SEM and TEM), static volumetric adsorption of helium and nitrogen, mercury porosimetry, Fourier transform infrared (FTIR) and atomic absorption spectroscopy (AAS), and elemental analysis. Mgt.PHEMA microspheres were coupled with p46/Myo1C protein purified from blood serum of multiple sclerosis (MS) patients, which enabled easy isolation of monospecific anti-p46/Myo1C immunoglobulin G (IgG) antibodies from crude antibody preparations of mouse blood serum. High efficiency of this approach was confirmed by SDS/PAGE, Western blot, and dot blot analyses. The newly developed mgt.PHEMA microspheres conjugated with a potential disease biomarker, p46/Myo1C protein, are thus a promising tool for affinity purification of antibodies, which can improve diagnosis and treatment of MS patients.

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Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus

Abstract: Affinity isolation of anti-histone immunoglobulins from blood serum of systemic lupus erythematosus patients using histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres (IO-iron oxide, HIS-histone).

Cited by 5 publications

References 31 publications

Antifouling Peptide Dendrimer Surface of Monodisperse Magnetic Poly(glycidyl methacrylate) Microspheres

Antifouling Peptide Dendrimer Surface of Monodisperse Magnetic Poly(glycidyl methacrylate) Microspheres

Benlysta-Loaded Sodium Alginate Hydrogel and Its Selective Functions in Promoting Skin Cell Growth and Inhibiting Inflammation

Magnetic poly(2-hydroxyethyl methacrylate) microspheres for affinity purification of monospecific anti-p46 kDa/Myo1C antibodies for early diagnosis of multiple sclerosis patients

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