Callus Culture for the Production of Therapeutic Compounds

Benjamin, Emmanuel Dabuwar; Ishaku, Gali Adamu; Peingurta, Fartisincha Andrew; Afolabi, A.

doi:10.11648/j.ajpb.20190404.14

Cited by 29 publications

(9 citation statements)

References 61 publications

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“…In callus culture, organogenesis occurs in two stages. first stage, formation of meristems takes place and in second stage, active growth of stem buds and roots takes place [21].…”

Section: Callus Culturementioning

confidence: 99%

Tissue Culture: Aeon of Micro Propagation in Vegetable Crops

Chaudhari

2023

IJECC

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Tissue culture in vegetable crops is a technique that has revolutionized the way we produce and propagate plants. It involves the growth of plant cells, tissues or organs in an artificial nutrient medium under sterile conditions. This method offers numerous advantages, including the production of disease-free plants, rapid multiplication and the ability to grow plants year-round. One of the key benefits of tissue culture in vegetable crops is the production of disease-free plants. By starting with a small piece of healthy tissue such as a leaf or stem, it is possible to grow an entire plant that is free from any pathogens. This is particularly important in vegetable crops, as diseases can significantly reduce yields and quality. By using tissue culture, farmers can ensure that the plants they grow are healthy and resistant to common diseases. Another advantage of tissue culture is the rapid multiplication of plants. Through a process called micropropagation, a single piece of tissue can be used to produce hundreds or even thousands of identical plants within a short period. This is particularly useful for vegetable crops that have a high demand or are difficult to propagate through traditional methods. By using tissue culture, farmers can quickly and efficiently produce large quantities of plants to meet market demands. Furthermore, tissue culture allows for year-round plant production. Unlike traditional methods that are limited by seasonal variations, tissue culture can be done in controlled environments such as laboratories or greenhouses. This means that farmers can grow vegetable crops regardless of the weather conditions outside. This is particularly advantageous for regions with harsh climates or limited growing seasons.

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“…In callus culture, organogenesis occurs in two stages. first stage, formation of meristems takes place and in second stage, active growth of stem buds and roots takes place [21].…”

Section: Callus Culturementioning

confidence: 99%

Tissue Culture: Aeon of Micro Propagation in Vegetable Crops

Chaudhari

2023

IJECC

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“…Kultur in vitro merupakan salah satu teknik mikropropagasi yang dapat digunakan untuk budidaya secara masal dan cepat (Benjamin et al, 2019). Salah satu teknik dalam kultur in vitro yang sering digunakan untuk mikropropagasi adalah induksi kalus yang mampu menghasilkan sel-sel yang bersifat meristematik (Efferth, 2019).…”

Section: Pendahuluanunclassified

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Marisa

Hidayati

Sasongko

et al. 2021

JBT

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Gyrinops versteegii is an endemic plant in eastern Indonesia that produced agarwood with high quality and economic value. This plant has been threatened by overexploitation which leads on decreasing in the natural population. This research aimed to induce in vitro callus formation to support the sustainable utilization of G. versteegii, which in the end, may support plant productivity. Callus induction was investigated using cotyledon and cultured on MS medium supplemented with several combination of plant growth regulators. The observations were done for the number of explants turned into callus and the duration for the first callus formation. The results showed that combination of 3 mg/L NAA + 0.5 mg/L BAP was recorded as the best combination for callus induction (63.63%). Callus with friable structure and bright color are obtained within nine days of incubation and showed the characteristic of embryonic callus. This result is expected to give a significant opportunity to conserve the natural population of G. versteegii.

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“…To overcome this problem, callus cultures can be used for the industrial production of rare or endangered plants, as well as those that are difficult to secure because of their small size or small number [10]. Callus cultures can produce specialized metabolites at a faster Plants 2024, 13, 167 2 of 15 rate than that of traditional cultivation and are performed under controlled conditions without being affected by various environmental factors (seasonal changes, microbial diseases, pests, and geographical constraints); therefore, their functional substances are continuously produced with consistent quality [11].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Reveals Rubiadin Accumulation and the Effects of Methyl Jasmonate Elicitation in Damnacanthus major Calli

Hyeon,

Jang,

Kim

et al. 2024

Plants

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Callus suspension techniques have been considered attractive for improving bioactive metabolite productivity; methyl jasmonate (MeJA) is a widely used elicitor for stimulating synthetic pathways. In this study, a multivariate analysis-based metabolomics approach was employed to investigate the primary and specialized metabolites in the leaves, unelicited calli, and 100 or 200 μM MeJA elicited calli of Damnacanthus major. Rubiadin, a powerful anthraquinone with various therapeutic properties, was only identified in D. major calli, accumulating in a MeJA elicitation concentration-dependent manner. Callus cultures also contained high levels of amino acids, sugars, and phenolic compounds, indicating energy metabolism and metabolic adaptation responses for proliferation and stabilization. Regarding MeJA application, elicited calli contained higher amounts of quinic acid, kaempferol, and glucose with lower amounts of sucrose and raffinose than those in the unelicited control, which were closely related to protective mechanisms against MeJA. Moreover, excessive elicitation increased the asparagine, fructose, and raffinose levels and decreased the glucose and sucrose levels, which was ascribed to increased activation of the aminoacyl-tRNA biosynthesis pathway and wider utilization of glucose than of fructose after sucrose degradation. These results will be useful for optimizing plant cell culture techniques to achieve high production rates for valuable specialized metabolites.

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Callus Culture for the Production of Therapeutic Compounds

Cited by 29 publications

References 61 publications

Tissue Culture: Aeon of Micro Propagation in Vegetable Crops

Tissue Culture: Aeon of Micro Propagation in Vegetable Crops

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Metabolomics Reveals Rubiadin Accumulation and the Effects of Methyl Jasmonate Elicitation in Damnacanthus major Calli

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