Callus Cultures

Neumann, K.–H.; Imani, Jafargholi

doi:10.1007/978-3-030-49098-0_3

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Several researches have been conducted to improve the production of secondary metabolites. Callus culture and cell suspension remain the foundation of the majority of these investigations [32]. Secondary metabolites have a wide range of uses and carry out several processes.…”

Section: Callus Culture and Secondary Metabolitesmentioning

confidence: 99%

Plant Tissue Culture and Formation of Secondary Metabolites - A Review

Ikram,

Sehar,

Khurshid

et al. 2023

BioSci Rev.

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Many copies of a single plant can be grown using the plant tissue culture technology. These copies have the right characteristics to satisfy medical and nutritional demands. Secondary metabolites are purposefully synthesized by using the in vitro technique. These metabolites act as protectors for plants during stressful conditions and offer resistance against different organisms and factors, ultimately helping the plant to survive. With the passage of time, the development of new instruments for the improved synthesis of secondary metabolites via the genetic control of biosynthetic pathways has been aided by the speedy development of recombinant DNA technology. Plants generate a wide range of secondary metabolites that have various biological functions, such as fungicide, herbicide, anti-parasitic, and anti- microbial functions. Nanotechnology has the potential to drastically alter conventional plant growing methods and bring about the synthesis of flavonoids, anthocyanin, and diosgenin by using silver nanoparticles and cadmium oxide nanoparticles (CdONPs). The technique of callus cultures is increasingly utilized to produce secondary metabolites. Hence, the main objective of the current review is to increase the synthesis of secondary metabolites.

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Section: Callus Culture and Secondary Metabolitesmentioning

confidence: 99%

Plant Tissue Culture and Formation of Secondary Metabolites - A Review

Ikram,

Sehar,

Khurshid

et al. 2023

BioSci Rev.

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show abstract

“…Comparison to Alternative Technology: Given a typical product value of less than a dollar per plant, achieving economic feasibility for axenic tissue culture methods is a tremendous challenge. Since the rst report of a Temporary Immersion System, the Auxophyton by Cornell's Steward Lab in 1952, signi cant productivity gains, and in turn scal costs (per plant), have been realized; an analysis of sugarcane in vitro production was reported to reduce production costs by 46% in 2002 (Neumann et al, 2020a(Neumann et al, , 2020b. Nonetheless, in vitro production of pineapple in a Periodic Immersion Bioreactor (PIB) reported in 2003 was still cost-prohibitive with a 500-fold increase in production despite a mere 35% cost increase.…”

Section: Signi Cance Of Bioreactor Operational Improvementsmentioning

confidence: 99%

“…roller bottles (Shetty, 2005)), mist bioreactors or nutrient sprinkle reactors (Steingroewer et al, 2013), and Temporary Immersion Bioreactors (TIBs). Existing TIBs include Temporary Root zone Immersion (TRI) bioreactors (Neumann et al, 2020a), Periodic Immersion Bioreactor (PIB), Plantform™ (Ruta et al, 2020), and the twin ask bioreactor system commercialized as the RITA® / SETIS™ (Georgiev et al, 2013). However, due to design constraints that limit economic feasibility (Eibl et al, 2018) these solutions have generally been relegated to research environments (Balogun et al, 2017) and commercial production of high-margin plant medicinal products and luxury crops (Ducos et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor

Tauger

Hile

Sreenivas

et al. 2021

Preprint

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In-vitro plant propagation systems such as Temporary Immersion Bioreactors (TIBs) are valuable tools that enable production of disease-free plants with improved traits. However, TIB systems can be expensive, difficult to implement, and prone to contamination due to sugar rich propagation media. Using rapidly growing chicory root cultures to expedite design-build-test cycles, we report here an improved, low-cost version of a previously reported Hydrostatically-driven TIB (Hy-TIB) that facilitates economical use of gas mixtures. Bioreactor improvements include decreased material costs, expanded modes of operation, and a horizontal orientation of a plastic film plant growth chambers that increase propagule light exposure. To take advantage of these improvements, we describe here experiments that evaluate the impacts of elevated CO2 on propagation of cacao (Theobroma cacao) secondary embryos and nodal cultures of yam (Dioscorea spp.) during both phototrophic and photomixotrophic growth. Our experiments show that elevated CO2 during plant propagation significantly improved both cacao and yam propagule development and eliminated the need for supplemental sugars in tissue culture growth media. Thus, our improved Hy-TIB shows potential as a simple, low-cost, and scalable propagation platform with cost-effective gas composition control and reduced risk of contamination overgrowth. We provide detailed instructions for assembly of this Hy-TIB design and discuss the implications of its adoption in food-insecure regions of the world.

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CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor

Trauger

Hile

Sreenivas

et al. 2022

Plant Cell Tiss Organ Cult

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In vitro plant propagation systems such as temporary immersion bioreactors (TIBs) are valuable tools that enable production of disease-free plants with improved traits. However, TIB systems can be expensive, difficult to implement, and prone to contamination due to sugar rich propagation media. Using rapidly growing chicory root cultures to expedite design-build-test cycles, we report here an improved, low-cost version of a previously reported Hydrostatically-driven TIB (Hy-TIB) that facilitates economical use of gas mixtures. Bioreactor improvements include decreased material costs, expanded modes of operation, and a horizontal orientation of a plastic film plant growth chambers that increase propagule light exposure. To take advantage of these improvements, we describe here experiments that evaluate the impacts of elevated CO2 on propagation of cacao (Theobroma cacao) secondary embryos and nodal cultures of yam (Dioscorea spp.) during both phototrophic and photomixotrophic growth. Our experiments show that elevated CO2 during plant propagation significantly improved both cacao and yam propagule development and eliminated the need for supplemental sugars in tissue culture growth media. Thus, our improved Hy-TIB shows potential as a simple, low-cost, and scalable propagation platform with cost-effective gas composition control and reduced risk of contamination overgrowth. We provide detailed instructions for assembly of this Hy-TIB design and discuss the implications of its adoption in food-insecure regions of the world.

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Callus Cultures

Cited by 4 publications

References 25 publications

Plant Tissue Culture and Formation of Secondary Metabolites - A Review

Plant Tissue Culture and Formation of Secondary Metabolites - A Review

CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor

CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor

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