Callus formation and cucurbitacin B accumulation in Ecballium elaterium callus cultures

Toker, Gülnur; Memişoğlu, Merve; Toker, MC; Yeşilada, Erdem

doi:10.1016/s0367-326x(03)00165-5

Cited by 19 publications

(14 citation statements)

References 6 publications

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“…E. elaterium is well known as a medicinal plant in Tunisia, frequently consumed in infusion, mixture of fruit or even in aerosol in cases of fever or flu (Bizid et al, 2014). This plant has been widely used in traditional medicine to treat various health disorders and diseases, including constipation, rheumatism, and jaundice (Toker et al, 2003;Attard et al, 2005;Bohlooli et al, 2012), hepatoprotective and proapoptotic effect (El Naggar et al, 2015). The fresh juice fruit has also been applied into the nostrils for the treatment of sinusitis, chronic jaundice and rheumatism (Jaradat et al, 2012;Sargin et al, 2013;Salhab, 2013).…”

Section: Introductionmentioning

confidence: 99%

Nutritional, phytochemical and antioxidant evaluation and FT-IR analysis of freeze dried extracts of Ecballium elaterium fruit juice from three localities

et al. 2016

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This study was designed to investigate chemical composition, the total phenolic content, flavonoid content, antioxidant activity and to analyze through FT-IR spectroscopy method the freeze-dried extract of Ecballium elaterium fruit from three different localities. The highest level of phenolic and flavonoid contents was recorded for the fruit juice from the Cap-Bon region, with 106.4 ± 0.4 mg GAE/g and 6.5 ± 0.2 mg QE/g, respectively. Antioxidant activity varied in dose-dependent manner with IC 50 values for DPPH scavenging of the freeze-dried fruit juice extracts from Cap-Bon, Kef and Sidi Bouzid were 38.6 ± 0.2, 50.1 ± 0.7, and 50.7 ± 0.2 µg/mL, respectively. The results from the FRAP test showed that the freeze-dried extracts of Cap-Bon exhibited potent activity, followed by those from Kef and Sidi Bouzid. Similar trend were revealed for ABTS•+ test, with the fruit juice extract from Cap-Bon (IC 50 = 0.6 ± 0.0 mg/mL). Furthermore, a good positive correlation was observed between the total phenols and three assays, especially DPPH. The freeze-dried extracts of fruit juice from Cap-Bon showed strong ability to act as antioxidants and can be considered as promising natural source of bioactive compounds. FT-IR analysis of each freeze-dried extract confirmed its richness on polyphenols and biological active functional groups.

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Section: Introductionmentioning

confidence: 99%

Nutritional, phytochemical and antioxidant evaluation and FT-IR analysis of freeze dried extracts of Ecballium elaterium fruit juice from three localities

et al. 2016

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“…The ratio and type of growth regulators used are very crucial factors for the stimulation of bio-synthetic pathways (Matkowski 2008). For example, the use of high concentration of 6-Benzylaminopurine (BAP, 1.0 mg/L) over 1-Naphthaleneacetic acid (0.1 mg/L) for the stem node callus culture of Ecballium elaterium was found to enhance the production of cucurbitacin B compared with original plant material (Toker et al 2003). In spite of these numerous researches, there is no available study about the influence of using plant growth regulators especially 2,4-dichlorophenoxyacetic acid (2,4-D) and BAP on antioxidant activities and production of total phenols and ascorbic acid of hawthorn callus cultures.…”

Section: Introductionmentioning

confidence: 99%

Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia

et al. 2015

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The present research work describes the effects of 2,4-dichlorophenoxyacetic acid (2,4-D)/Ben-zylaminopurine (BAP) ratio on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leafderived calli of Crataegus azarolus (hawthorn). The supplementation of 1.0 mg/L 2,4-D and 1.0 mg/L BAP to MS medium was found to be the most efficient for callus induction (as percentage and fresh weigh). The results of biochemical analysis showed that the highest total phenolic contents were obtained in callus cultured on MS medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP (52 ± 0.56 mg GAE/g DM) and were significantly lower than those of intact leaves (76±1.72mg GAE/g DM). However, the highest ascorbic acid contents were found in callus cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 0.5 mg/L BAP (0.96 ± 0.13 mg AAE/g DM) and these amounts were statistically similar to those found in leaf tissues (0.74 ± 0.07 mg AAE/g DM). Antioxidant activities of callus extracts were determined using two TEAC assays and results showed that extract of callus cultured on MS medium supplemented with 2.0 mg/L 2,4-D and 1.0 mg/L BAP have the greatest antiradical activities against DPPH (124 ± 2.92 mg TE/g DM) and ABTS (0.19 ± 0.02 mg TE/g DM) compared to the leaves of field-grown plant. Thus, the use of high level of 2,4-D over BAP can be suitable to enhance the quality more than the quantity of bioactive compounds in leaf callus culture of hawthorn.

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“…, insecticidal 5,12 and inhibiting cancer cell growth 13,14 . However, thus so far there is no phytochemistry reported from K. prolifera.…”

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confidence: 99%

Flavonoid Compounds from the Leaves ofKalanchoe proliferaand Their Cytotoxic Activity against P-388 Murine Leukimia Cells

et al. 2017

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− Seven flavonoid compounds, kaempferol (1), quercetin (2), quercetin (6) and quercetin-3-O-rutinoside (7), were isolated from the methanolic extract of leaves of Kalanchoe prolifera. Compounds 1-7 were isolated for first time from this plant. These compounds were evaluated their cytotoxic activity against P-388 murine leukimia cells in vitro. Among those compounds kaempferol (1) and quercetin (2) showed strongest cytotoxic activity with IC 50 values of 4.45 ± 0.05 and 6.28 ± 0.02 μg/mL, respectively.

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Callus formation and cucurbitacin B accumulation in Ecballium elaterium callus cultures

Cited by 19 publications

References 6 publications

Nutritional, phytochemical and antioxidant evaluation and FT-IR analysis of freeze dried extracts of Ecballium elaterium fruit juice from three localities

Nutritional, phytochemical and antioxidant evaluation and FT-IR analysis of freeze dried extracts of Ecballium elaterium fruit juice from three localities

Effects of 2,4-dichlorophenoxyacetic acid combined to 6-Benzylaminopurine on callus induction, total phenolic and ascorbic acid production, and antioxidant activities in leaf tissue cultures of Crataegus azarolus L. var. aronia

Flavonoid Compounds from the Leaves ofKalanchoe proliferaand Their Cytotoxic Activity against P-388 Murine Leukimia Cells

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