Callus induction and indirect regeneration of Thunbergia coccinea Wall.

Sultana, Kaniz Wahida; Chandra, Indrani; Roy, Aniruddha

doi:10.1007/s40502-020-00501-z

Cited by 5 publications

(3 citation statements)

References 17 publications

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“…2 f). The survival rate for the regenerated plantlets was increased to 70% from the previous study of T. coccinea 28 .…”

Section: Resultsmentioning

confidence: 84%

“…Inadequacy of seeds, low germination rate, and unavailability of the fruit of this plant necessitate the establishment of an efficient protocol for in vitro propagation. The previous study was about the indirect regeneration of Thunbergia coccinea through somatic embryogenesis from leaf callus 28 , while the present effort is to propagate the plant by direct multiple shoot induction from nodal segments under in vitro conditions. Since there is no report on in vitro propagation of the plant via direct shoot multiplication.…”

Section: Introductionmentioning

confidence: 99%

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Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Sultana

Das

Chandra

et al. 2022

Sci Rep

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Thunbergia coccinea Wall. ex D. Don being a rare, ornamental and medicinal plant of India, is needed to propagate for conserving the germplasm and analyzing its phytochemical compounds in the future. A reliable protocol for direct in vitro propagation using nodal shoot meristem of T. coccinea as explant was standardized. The highest number of shoots per explant (22.17 ± 0.54) with maximum shoot length (2.36 ± 0.28) in cm was obtained in Murashige and Skoog (MS) medium supplemented with 9.70 µM of 6-furfurylaminopurine (Kinetin) and 0.053 µM of α-naphthaleneacetic acid (NAA) combination, among all the different plant growth regulators (PGR’s) and concentrations tested. The aforesaid PGR’s combination was optimum for axillary shoot bud induction and multiplication in T. coccinea. The best rooting was observed on the half-strength MS medium fortified with 2.68 µM NAA with the highest number of roots per shoot (3.75 ± 0.12) and maximum length (5.22 ± 0.32) in cm. All the in vitro raised plantlets were acclimatized in sterile sand and soil mixture (1:1) with a survival rate of 70% on earthen pots under greenhouse conditions. PCR-based RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat) molecular markers were employed to determine the genetic homogeneity amongst the plantlets. Twelve (12) RAPD and nine (9) ISSR primers developed a total of 104 and 91 scorable bands, respectively. The band profiles of micropropagated plantlets were monomorphic to the mother, donor in vivo plant, and similarity values varied from 0.9542–1.000. The dendrogram generated through UPGMA (unweighted pair group method with arithmetic mean) showed 99% similarities amongst all tested plants confirming the genetic uniformity of in vitro raised plants.

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“…2 f). The survival rate for the regenerated plantlets was increased to 70% from the previous study of T. coccinea 28 .…”

Section: Resultsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Sultana

Das

Chandra

et al. 2022

Sci Rep

Self Cite

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“…Shoot tips were excised from the plant and used as the explant source. Explants were washed in Milli-Q water (Millipore system, Merck, Rahway, NJ, USA) for 5 min, then disinfected in 70% ethanol v / v for 10 s followed by washing with 0.01% v / v Tween-20 for 4 min [ 48 , 49 ]. Surface sterilization was performed using 0.1% w / v mercuric chloride for 45 s in a Biosafety Cabinet A2 (Biobase Inc., Jinan, China) under aseptic conditions.…”

Section: Methodsmentioning

confidence: 99%

Unveiling the Dual Nature of Heavy Metals: Stressors and Promoters of Phenolic Compound Biosynthesis in Basilicum polystachyon (L.) Moench In Vitro

Das,

Sultana,

Mondal

et al. 2023

Plants

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The global industrial revolution has led to a substantial rise in heavy metal levels in the environment, posing a serious threat to nature. Plants synthesize phenolic compounds under stressful conditions, which serve as protective agents against oxidative stress. Basilicum polystachyon (L.) Moench is an herbaceous plant of the Lamiaceae family. Some species within this family are recognized for their capacity to remediate sites contaminated with heavy metals. In this study, the effects of mercury (II) chloride and lead (II) nitrate on the in vitro propagation of B. polystachyon were investigated. Shoot tips from in vitro plantlets were cultured in Murashige and Skoog’s (MS) media with heavy metals ranging from 1 to 200 µM to induce abiotic stress and enhance the accumulation of phenolic compounds. After three weeks, MS medium with 1 µM of lead (II) supported the highest shoot multiplication, and the maximum number of roots per explant was found in 100 µM of lead (II), whereas a higher concentration of heavy metals inhibited shoot multiplication and root development. The plantlets were hardened in a greenhouse with a 96% field survival rate. Flame atomic absorption spectroscopy (FAAS) was used to detect heavy metal contents in plant biomass. At both 200 µM and 50 µM concentrations, the greatest accumulation of mercury (II) was observed in the roots (16.94 ± 0.44 µg/g) and shoots (17.71 ± 0.66 µg/g), respectively. Similarly, lead (II) showed the highest accumulation in roots (17.10 ± 0.54 µg/g) and shoots (7.78 ± 0.26 µg/g) at 200 µM and 50 µM exposures, respectively. Reverse-phase high-performance liquid chromatography (RP-HPLC) identified and quantified various phenolic compounds in B. polystachyon leaves, including gallic acid, caffeic acid, vanillic acid, p-coumaric acid, ellagic acid, rosmarinic acid, and trans-cinnamic acid. These compounds were found in different forms, such as free, esterified, and glycosylated. Mercury (II)-exposed plants exhibited elevated levels of vanillic acid (1959.1 ± 3.66 µg/g DW), ellagic acid (213.55 ± 2.11 µg/g DW), and rosmarinic acid (187.72 ± 1.22 µg/g DW). Conversely, lead (II)-exposed plants accumulated higher levels of caffeic acid (42.53±0.61 µg/g DW) and p-coumaric acid (8.04 ± 0.31 µg/g DW). Trans-cinnamic acid was the predominant phenolic compound in control plants, with a concentration of 207.74 ± 1.45 µg/g DW. These results suggest that sublethal doses of heavy metals can act as abiotic elicitors, enhancing the production of phenolic compounds in B. polystachyon. The present work has the potential to open up new commercial opportunities in the pharmaceutical industry.

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Adventitious rhizogenesis in Basilicum polystachyon (L.) Moench callus and HPLC analysis of phenolic acids

2021

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Callus induction and indirect regeneration of Thunbergia coccinea Wall.

Cited by 5 publications

References 17 publications

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Unveiling the Dual Nature of Heavy Metals: Stressors and Promoters of Phenolic Compound Biosynthesis in Basilicum polystachyon (L.) Moench In Vitro

Adventitious rhizogenesis in Basilicum polystachyon (L.) Moench callus and HPLC analysis of phenolic acids

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