Callus induction and plant regeneration from immature zygotic embryos of various maize genotypes (Zea mays L.)

Abstract

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract We investigated the callus induction and plant regeneration ability of 16 maize genotypes, including the Korean inbred lines, using 9 to 15 day-old immature zygotic embryos from maize grown in pots and from field cultures.

A New Protocol to Mitigate Damage to Germination Caused by Black Layers in Maize (Zea mays L.) Seeds

“…In this study, the KS140 and KS141 varieties were obtained from the NICS (National Institute of Crop Science, Rural Development Administration, 181 Hyeoksin-ro, Iseo-myeon, Wanju-Gun, Jeollabuk-do, Republic of Korea), the HW3 variety was procured from the MES (Maize Experiment Station, Gangwondo Agricultural Research and Extension Services, Republic of Korea), the A188, B73, B98, B104, H99, Hi IIA, and Hi IIA × Hi IIB varieties were acquired from the USDA (US Department of Agriculture) [33], and two F1 hybrid varieties (Hi IIA(♂) × B73(♀) and B73(♂) × Hi IIA(♀)) were produced through Hi IIA and B73 hybridization [32], with the 12 total varieties used for seed sterilization.…”

“…In addition, germination in the 50-hole pot was based on the appearance of seedlings of more than 5 mm, and germination was investigated from the second day to the seventh after transfer to the soil. Pollination was carried out at 18-22 • C between 8:00 and 10:00 in the morning, which is a timeframe known to have an optimal pollination rate in field conditions (Weather Spark, https://weatherspark.com/y/142288/Average-Weather-in-Jeonju-South-Korea-Year-Round, accessed on 2 January 2021) between June and July in Jeonju, Republic of Korea [33]. Although there are slight differences depending on the variety and environment, mature seeds were generally harvested from July to August (Figure S1).…”

Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

“…One of the directions in the development of plant biotechnologies using calli is the identification of the relationship between the content of endogenous (in explant or callus) and the concentration of exogenous (in a culture medium) hormones for regulating morphogenesis pathways in vitro. Such studies are not numerous and they were carried mainly on cereals [12][13][14]. Based on the available literature, studies of endogenous hormones accumulation in order to identify their participation in the callusogenesis and the regulation of morphogenesis in callus cultures in vitro for lavender have not been previously conducted.…”