Calorie restriction‐induced maternal longevity is transmitted to their daughters in a rotifer

Kaneko, Gen; Yoshinaga, Tatsuki; Yanagawa, Yoshiko; Ozaki, Yori; Tsukamoto, Katsumi; Watabe, Shugo

doi:10.1111/j.1365-2435.2010.01773.x

Cited by 54 publications

(55 citation statements)

References 37 publications

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“…As a natural stress, moderate starvation has been reported to enhance the expression of many genes, including SOD (Kaneko et al 2005;Kaneko et al 2011), catalase (CAT) , hsp70, and GRP94 (Kaneko et al 2002). DR is the most common starvation method for prolonging life in the laboratory, and it has been reported to extend the lifespan of different organisms (Fanestil and Barrows 1965;Klass 1977;Lin et al 2002;Walker et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

Yang

Dong

et al. 2014

Cell Stress and Chaperones

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Heat shock proteins (HSPs) are molecular chaperones and have an important role in the refolding and degradation of misfolded proteins, and these functions are related to aging. Rotifer is a useful model organism in aging research, owing to small body size (0.1-1 mm), short lifespan (6-14 days), and senescence phenotypes that can be measured relatively easily. Therefore, we used rotifer as a model to determine the role of four typical hsp genes on the aging process in order to provide a better understanding of rotifer aging. We cloned cDNA encoding hsp genes (hsp40, hsp60, hsp70, and hsp90) from the rotifer Brachionus calyciflorus Pallas, analyzed their molecular characteristics, determined its modulatory response under different temperatures and H 2 O 2 concentrations and investigated the changes in expression of these genes during the aging process. We found that Bchsp70 mRNA expression significantly decreased with aging. In addition, we also studied the effects of dietary restriction (DR) and vitamin E on rotifer lifespan and reproduction and analyzed the changes in expression of these four Bchsp genes in rotifers treated with DR and vitamin E. The results showed that DR extended the lifespan of rotifers and reduced their fecundity, whereas vitamin E had no significant effect on rotifer lifespan or reproduction. Real-time PCR indicated that DR increased the expression of these four Bchsps. However, vitamin E only improved the expression of Bchsp60, and reduced the expression of Bchsp40, Bchsp70, and Bchsp90. DR pretreatment also increased rotifer survival rate under paraquat-induced oxidative stress. These results indicated that hsp genes had an important role in the anti-aging process.

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Section: Discussionmentioning

confidence: 99%

Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

Yang

Dong

et al. 2014

Cell Stress and Chaperones

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“…Details of the procedure for immunocytochemical staining are described in Kaneko et al [16]. Rotifers treated with 1% ethanol were used as controls.…”

Section: Statistical Analysesmentioning

confidence: 99%

“…The synthesized cDNAs were diluted 50 times with sterilized, distilled water and used as the template for quantitative real-time PCR. Two sets of the gene-specific primers of RT-Mn-F (5 0 -CC GCGTGTGCTAACCAAGA-3 0 )/RT-Mn-R (5 0 -TTCCCAA ACATCAATGCCAA-3 0 ) and rCAT-qRT-F1 (5 0 -CAACGT GATGGCCCACAAT-3 0 )/rCAT-qRT-R1 (5 0 -GGTCCAGA GAAAGAATTCGGATAA-3 0 ) were used for quantitative real-time PCR for the Mn SOD and catalase genes, respectively [16,20]. Quantitative real-time PCR was carried out using SYBR Premix Ex Taq (Takara, Otsu, Japan) and the ABI PRISM 7300 real-time PCR system (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

“…CR induces resistance to oxidative stress evoked by paraquat (1,1-dimethyl-4,4-bipyrimidyl chloride), an exogenous ROS generator [15], whereas the relative mRNA levels of Mn SOD and catalase are enhanced in the rotifer under normal physiological conditions without oxidative stress [16]. However, it is still not known whether CR affects the expressions of these antioxidant enzymes under oxidative stress conditions.…”

Section: Introductionmentioning

confidence: 99%

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Effects of calorie restriction on the expression of manganese superoxide dismutase and catalase under oxidative stress conditions in the rotifer Brachionus plicatilis

Kailasam

Kaneko

et al. 2011

Fish Sci

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Calorie restriction (CR) in the rotifer Brachionus plicatilis extends its lifespan, as it enhances the expression of antioxidant enzymes such as manganese superoxide dismutase (Mn SOD) and catalase. Here we show that CR also increased the mRNA levels of these antioxidant enzymes upon exposure to oxidative stress. Rotifers cultured under CR showed a higher survival rate than those fed ad libitum (AL) upon exposure to 0.05-0.2 lM juglone, an oxidative stress inducer. The relative mRNA levels of Mn SOD and catalase before exposure to juglone were slightly higher in the CR rotifers than in their AL counterparts, although these differences were not statistically significant. AL rotifers showed no apparent upregulation of the mRNA levels of these antioxidant enzymes upon exposure to 0.025 and 0.05 lM juglone. In contrast, the CR rotifers increased the mRNA levels of Mn SOD and catalase by up to 5.4-fold and 4.2-fold, respectively, resulting in significant differences between their levels in AL and CR rotifers under oxidative stress conditions. Furthermore, the protein level of catalase was clearly higher in CR than in AL rotifers 6 h after exposure to oxidative stress. These results suggest that the upregulation of Mn SOD and catalase genes is involved in CR-induced resistance to oxidative stress in the rotifer.

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“…They shift their growth from an early exponential to exponential growth phase and subsequently to a stationary phase, finally declining their population (senescent phase). Various studies have been conducted to disclose the molecular mechanisms involved in the growth of rotifers partly because the efficient culture of rotifers has been eagerly required at the industrial levels (Yu & Hirayama, 1986;Yamasaki et al, 1987;Yoshinaga et al, 1999;Koiso & Hino, 2002;Kaneko et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A novel growth-promoting protein in the conditioned media from the rotifer Brachionus plicatilis at an early exponential growth phase

et al. 2011

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We confirmed the existence of growthpromoting substances in the conditioned media (CM) from the rotifer Brachionus plicatilis at an early exponential growth phase and isolated a novel protein with a growth-promoting activity from the crude extract (CE) of rotifer cells. CM was prepared from the culture media where rotifers had been cultured at an early exponential growth phase and filtered through a 0.22-lm filter membrane. The growth-promoting activity was determined using rotifers in CM for 5 days. As a result, the increase of rotifers added with CM was significantly higher than that of the control in artificial seawater (P \ 0.001). Moreover, the growthpromoting activity of CM was dose-dependent and inactivated by heat treatment at 80°C for 60 min. Meanwhile, CM filtered through a \10 kDa ultrafiltration membrane showed a low activity, whereas proteinase K treatment resulted in a complete inactivation. These results suggest that the rotifer secrets growth-promoting proteins into CM. CE also contained a protein with the activity and properties similar to those found in CM. Then, CE was subjected to purification of a growth-promoting protein for convenience using various types of chromatography after fractionation with 30-80% saturated ammonium sulfate. Subsequently, a protein with an approximate molecular weight of 25000 was isolated, and its N-terminal amino acid sequence was determined to be PAVVDFTAVWFGPLQMIKP. An orthologue was found in the EST database of B. plicatilis, the full sequence of which showed about 50% identity to the corresponding regions of thioredoxins from other organisms.

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Calorie restriction‐induced maternal longevity is transmitted to their daughters in a rotifer

Cited by 54 publications

References 37 publications

Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

Effects of calorie restriction on the expression of manganese superoxide dismutase and catalase under oxidative stress conditions in the rotifer Brachionus plicatilis

A novel growth-promoting protein in the conditioned media from the rotifer Brachionus plicatilis at an early exponential growth phase

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