Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

Yang, Jianghua; Mu, Yawen; Dong, Siming; Jiang, Qichen; Yang, Jiaxin

doi:10.1007/s12192-013-0432-0

Cited by 26 publications

(16 citation statements)

References 84 publications

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“…The critical step is the synchronization of rotifer conditions over several generations. When experimental rotifers are well synchronized, a typical type I survival curve is observed with very little early mortality as reported in several previous studies 18,24,37,38 . Standard deviations of their survival time therefore become smaller compared to poorly synchronized rotifers, resulting in high statistical power.…”

Section: Discussionsupporting

confidence: 77%

Measurement of Survival Time in <em>Brachionus </em>Rotifers: Synchronization of Maternal Conditions

Kaneko

Yoshinaga

Gribble

et al. 2016

JoVE

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Rotifers are microscopic cosmopolitan zooplankton used as models in ecotoxicological and aging studies due to their several advantages such as short lifespan, ease of culture, and parthenogenesis that enables clonal culture. However, caution is required when measuring their survival time as it is affected by maternal age and maternal feeding conditions. Here we provide a protocol for powerful and reproducible measurement of the survival time in Brachionus rotifers following a careful synchronization of culture conditions over several generations. Empirically, poor synchronization results in early mortality and a gradual decrease in survival rate, thus resulting in weak statistical power. Indeed, under such conditions, calorie restriction (CR) failed to significantly extend the lifespan of B. plicatilis although CR-induced longevity has been demonstrated with well-synchronized rotifer samples in past and present studies. This protocol is probably useful for other invertebrate models, including the fruitfly Drosophila melanogaster and the nematode Caenorhabditis elegans, because maternal age effects have also been reported in these species.

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Section: Discussionsupporting

confidence: 77%

Measurement of Survival Time in <em>Brachionus </em>Rotifers: Synchronization of Maternal Conditions

Kaneko

Yoshinaga

Gribble

et al. 2016

JoVE

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“…This observation suggests an interesting hypothesis that treatments shifting metabolism from aerobic to anaerobic pathways could be as effective for life extension as CR. Extending this CR-stress resistance link to B. calyciflorus, Yang et al (2013) showed that CR increased expression of four heat shock genes (hsp 40, 60, 70, 90) and increased rotifer resistance to paraquat-induced oxidative stress.…”

Section: Discussionmentioning

confidence: 97%

Joint inhibition of TOR and JNK pathways interacts to extend the lifespan of Brachionus manjavacas (Rotifera)

Snell

Johnston

Rabeneck

et al. 2014

Experimental Gerontology

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The TOR kinase pathway is central in modulating aging in a variety of animal models. The target of rapamycin (TOR) integrates a complex network of signals from growth conditions, nutrient availability, energy status, and physiological stresses and matches an organism’s growth rate to the resource environment. Important problems remaining are to identify the pathways that interact with TOR and characterize them as additive or synergistic. One of the most versatile stress sensors in metazoans is the Jun-N-terminal Kinase (JNK) signalling pathway. JNK is an evolutionarily conserved stress-activated protein kinase that is induced by a range of stressors, including UV irradiation, reactive oxygen species, DNA damage, heat, and bacterial antigens. JNK is thought to interact with the TOR pathway, but its effects on TOR are poorly understood. We used the rotifer Brachionus manjavacas as a model animal to probe the regulation of TOR and JNK pathways and explore their interaction. The effect of various chemical inhibitors was examined in life table and stressor challenge experiments. A survey of 12 inhibitors revealed two, rapamycin and JNK inhibitor, that significantly extended lifespan of B. manjavacas. At 1 μM concentration, exposure to rapamycin or JNK inhibitor extended mean rotifer lifespan by 35% and maximum lifespan by 37%. Exposure to both rapamycin and JNK inhibitor simultaneously extended mean rotifer lifespan 65% more than either alone. Exposure to a combination of rapamycin and JNK inhibitors conveyed greater protection to starvation, UV and osmotic stress than either inhibitor alone. RNAi knockdown of TOR and JNK gene expression was investigated for its ability to extend rotifer lifespan. RNAi knockdown of the TOR gene resulted in 29% extension of mean lifespan compared to control and knockdown of the JNK gene resulted in 51% mean lifespan extension. In addition to lifespan, we quantified mitochondria activity using the fluorescent marker Mitotracker and lysosome activity using Lysotracker. Treatment of rotifers with JNK inhibitor enhanced mitochondria activity nearly 3-fold, whereas rapamycin treatment had no significant effect. Treatment of rotifers with rapamycin or JNK inhibitor reduced lysosome activity in 1, 3 and 8 day old animals, but treatment with both inhibitors did not produce any additive effect. We conclude that inhibition of TOR and JNK pathways significantly extends the lifespan of B. manjavacas. These pathways interact so that inhibition of both simultaneously acts additively to extend rotifer lifespan more than inhibition of either alone.

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“…is a freshwater planarian, characterized by more than a hundred eyes in the head (Aikawa and Shimozawa 1991). Compared with the two most commonly studied species of freshwater planarians, Dugesia japonica and Schmidtea mediterranea, it is more susceptible to various environmental stresses such as temperature variation, heavy metals, and toxic substance pollution; therefore, it is more suitable to be used as test organisms in water environmental toxicology (Li 2008;Knakievicz and Ferreira 2008;Gleixner et al 2014;Yang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of a heat-shock protein 70 gene in Polycelis sp.

Cheng

Dong

et al. 2015

Cell Stress and Chaperones

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Heat-shock protein 70 (HSP70) is ubiquitously found in a variety of organisms and plays an important role in cytoprotection, environmental monitoring, and disease resistance. In this study, the full-length complementary DNA (cDNA) of hsp70 from planarian Polycelis sp. was first cloned using rapid amplification of cDNA ends (RACE). The expression levels of Pyhsp70 were analyzed in the presence of various stressors by real-time PCR, and its temporal-spatial expression patterns were also examined in both intact and regenerative animals by whole-mount in situ hybridization. The results show that (1) the deduced amino acid sequence of Pyhsp70 includes three typical HSP70 family signature motifs and is highly conserved during evolution; (2) Pyhsp70 expression is induced by prolonged starvation, tissue damage, and ionic liquid but inhibited by high or low temperatures; and (3) Pyhsp70 mRNA is mainly expressed in the head peripheral region and in the regenerating blastema during regeneration. These results suggest that the highly expressed Pyhsp70 gene may contribute to enhance cytoprotection and tolerance against stress-induced molecular damage, and the migration of neoblasts to the wound, which might also be involved in the proliferation and differentiation of neoblasts. Our work provides basic data for the study of stress responses and regenerative mechanism in freshwater planarians.

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Changes in the expression of four heat shock proteins during the aging process in Brachionus calyciflorus (rotifera)

Cited by 26 publications

References 84 publications

Measurement of Survival Time in <em>Brachionus </em>Rotifers: Synchronization of Maternal Conditions

Measurement of Survival Time in <em>Brachionus </em>Rotifers: Synchronization of Maternal Conditions

Joint inhibition of TOR and JNK pathways interacts to extend the lifespan of Brachionus manjavacas (Rotifera)

Identification and expression analysis of a heat-shock protein 70 gene in Polycelis sp.

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