Measurement of Survival Time in &lt;em&gt;Brachionus &lt;/em&gt;Rotifers: Synchronization of Maternal Conditions

Kaneko, Gen; Yoshinaga, Tatsuki; Gribble, Kristin E.; Welch, David B. Mark; Ushio, Hideki

doi:10.3791/54126

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…We have developed straightforward and multifaceted methods that support quantifiable in vivo toxicological testing on rotifers. While there have been previous assays developed for the lifespan and survival of rotifers (Kaneko et al, 2016), these have been on the basis of assaying large groups of aggregated animals. A caveat with this approach is that the dead animals may serve as food sources for the living ones.…”

Section: Discussionmentioning

confidence: 99%

Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer

Oláh

Bush

Aleksza

et al. 2017

Ecotoxicology and Environmental Safety

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Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid rotifer model to use in high-throughput viability and non-invasive assays. In order to identify our species Philodina acuticornis odiosa (PA), 18S rDNA-based phylogenetic analysis was carried out and their species-specific morphological markers identified. To execute the rotifer-based experiments, we developed an oil-covered water-drop methodology adapted from human in vitro fertilization techniques. This enables toxicological observations of individual one-housed rotifers in a closed and controllable micro-environment for up to several weeks. Hydrogen peroxide (HO) and sodium azide (NaN) exposures were used as well-understood toxins. The toxicity and survival lifespan (TSL), the bright light disturbance (BLD) the mastax contraction frequency (MCF) and the cellular reduction capacity (CRC), indices were recorded. These newly developed assays were used to test the effects of lethal and sublethal doses of the toxins. The results showed the expected dose-dependent decrease in indices. These four different assays can either be used independently or as an integrated system for studying rotifers. These new indices render the PA invertebrate rotifer model a quantitative system for measuring viability, toxicity and lifespan (with TSL), systemic reaction capacity (with BLD), organic functionality (with MCF) and reductive capability of rotifers (with CRC), in vivo. This novel multi-level system is a reliable, sensitive and replicable screening tool with potential application in pharmaceutical science.

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Section: Discussionmentioning

confidence: 99%

Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer

Oláh

Bush

Aleksza

et al. 2017

Ecotoxicology and Environmental Safety

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“…Rotifers were fed with 5×10 6 cell/mL Chlorella pyrenoidesa , and the medium was replaced every day. Before experiment, some rotifer with amictic egg were chosen from clone strain and synchronized (Kaneko et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Carryover effects of tire wear particle leachate threats reproduction across multiple generations

Chai

Wang

et al. 2022

Preprint

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Toxic additives leached from tire wear particle (TWP) have been linked to some collective death events of fish, also impose impacts on zooplankton as secondary consumer in aquatic food web. In addition to direct impacts of TWP leachate at the current generation, potential delayed carryover from past exposure across multi-generational lineage may augment impacts on individual reproduction, then population maintenance. We investigated the carryover effects from persistent exposure and past TWP leachate exposure along generation passaging on the individual reproduction of rotifer, Brachionus calyciflorus, a typical zooplankton. For rotifer treated with TWP leachate across continuous 7 generations, their offspring were divided into parental exposure or no exposure in each generation. And rotifer transferred into no exposure were maintained for 3 generations to eliminate indirect exposure through their parental germ. The similar response of reproduction, via carryover effects from parental exposure, also emerged in offspring without exposure. Persistent exposure across multiple generations additively impaired individual reproduction performance by transferring from its hermetic effects in original generation, even caused final population collapse. TWP leachate could impose cascading toxicity on population persistence of zooplankton via carryover and cumulative effects on reproduction for long term, which must be considered in risk assessment and management policy to alleviate the effects of TWP.

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“…The cytochrome oxidase subunit I gene sequence has been registered for detailed identification of this strain (GenBank accession number LC422762). Rotifers were cultured at 25 ˚C by a standard protocol (Kaneko et al 2016) using half-diluted Brujewicz artificial seawater and fed the algae Chlorella regularis (Nikkai Center, Tokyo, Japan) at a final concentration of approximately 7 × 10 6 cells/mL. See supplementary material 2 for details of molecular characterization of rotifer HSP70 genes.…”

Section: Culturementioning

confidence: 99%

The complex evolution of the metazoan HSP70 gene family

Yoshinaga

Jalufka

et al. 2020

Preprint

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The 70-kDa heat shock protein (HSP70) family contains several isoforms localized in different subcellular compartments. The cytosolic isoforms have been classified into stress-inducible HSP70s and constitutive heat shock cognates (HSC70s), but occasional reports of “constitutive HSP70s” and the lack of cross-phylum comparisons have been a source of confusion in the evolution of the metazoan HSP70 family. Here we provide novel insights into the evolutionary history of this important molecular chaperone. We first cloned two HSP70 genes from the rotifer Brachionus plicatilis, an emerging model in evolutionary genetics, and confirmed their stress inducibility. Subsequent phylogenetic analyses of 100 full-length HSP70 family member genes revealed an ancient duplication that gave rise to two lineages from which all metazoan cytosolic HSP70s descend. One lineage contains a relatively small number of Lophotrochozoan and Ecdysozoan genes, none of which have been shown to be constitutively expressed. The second included both inducible and constitutive genes from diverse phyla. Species-specific duplications are present in both lineages, and in the second there are well-supported phylum-specific clades for Rotifera, Nematoda, and Chordata. Some genes in this lineage have likely independently acquired stress inducibility, which may explain the sporadic distribution of genes designated as “HSP70” or “HSC70” in previous analyses. Consistent with the history of diversification within each group, stress-inducible members of the second lineage show lower purifying selection pressure compared to constitutive members. These results illustrate the evolutionary history of the HSP70 family independent from their expression patterns, encouraging the development of new nomenclature based on evolutionary history.

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Measurement of Survival Time in <em>Brachionus </em>Rotifers: Synchronization of Maternal Conditions

Cited by 4 publications

References 31 publications

Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer

Novel in vivo experimental viability assays with high sensitivity and throughput capacity using a bdelloid rotifer

Carryover effects of tire wear particle leachate threats reproduction across multiple generations

The complex evolution of the metazoan HSP70 gene family

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