Calorimetric measurements of proton adsorption onto Pseudomonas putida

Gorman‐Lewis, Drew

doi:10.1016/j.jcis.2009.05.066

Cited by 10 publications

(5 citation statements)

References 36 publications

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“…Nearly all chemical reactions either produce or absorb heat. In terms of bacterial surface reactivity, the extent of heat produced is proportional to the extent of adsorption occurring (Gorman-Lewis et al, 2006, 2009. Calorimetric measurements are the only viable means to measure enthalpies of reaction on bacterial surfaces.…”

Section: Introductionmentioning

confidence: 99%

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Enthalpies of proton adsorption onto Bacillus licheniformis at 25, 37, 50, and 75°C

Gorman‐Lewis¹

2011

Geochimica et Cosmochimica Acta

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Section: Introductionmentioning

confidence: 99%

“…Isothermal calorimeters are also designed to be used at a wide range of temperatures, thus providing a technique to investigate temperature effects on bacterial adsorption reactions. ITC has been previously applied to adsorption reactions with mesophilic bacteria but never at temperatures other than 25°C (Gorman-Lewis et al, 2006, 2009.…”

Section: Introductionmentioning

confidence: 99%

Enthalpies of proton adsorption onto Bacillus licheniformis at 25, 37, 50, and 75°C

Gorman‐Lewis¹

2011

Geochimica et Cosmochimica Acta

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“…acidocaldarius to heterotrophs previously investigated with potentiometric and calorimetric titrations reveals surface site identities not previously inferred. Past work on heterotrophs revealed carboxylate, phosphonate, thiol, and amine reactivity similar to those of the species investigated here (Gorman‐Lewis et al ., ; Gorman‐Lewis, , ). No calorimetric surface reactivity data exist for species with S‐layers; however, Harrold and Gorman‐Lewis () determined the enthalpies of protonation of Bacillus subtilis ( B .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, application of different models to the proton uptake data might yield alternate conclusions. However, previous calorimetric investigations of microbial surface reactivity resulted in similar conclusions when testing different surface complexation models on the same dataset (Gorman‐Lewis et al ., ; Gorman‐Lewis, ). Although thermodynamic data cannot provide conclusive evidence of site identity, previous calorimetric investigations of surface reactivity are consistent with spectroscopic investigations of surface site identity, and a combined calorimetric/surface complexation modeling approach provides data that cannot be determined using other techniques (Gorman‐Lewis et al ., ; Gorman‐Lewis, ).…”

Section: Discussionmentioning

confidence: 99%

Thermodynamic characterization of proton‐ionizable functional groups on the cell surfaces of ammonia‐oxidizing bacteria and archaea

2014

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The ammonia-oxidizing archaeon Nitrosopumilus maritimus strain SCM1 (strain SCM1), a representative of the Thaumarchaeota archaeal phylum, can sustain high specific rates of ammonia oxidation at ammonia concentrations too low to sustain metabolism by ammonia-oxidizing bacteria (AOB). One structural and biochemical difference between N. maritimus and AOB that might be related to the oligotrophic adaptation of strain SCM1 is the cell surface. A proteinaceous surface layer (S-layer) comprises the outermost boundary of the strain SCM1 cell envelope, as opposed to the lipopolysaccharide coat of Gram-negative AOB. In this work, we compared the surface reactivities of two archaea having an S-layer (strain SCM1 and Sulfolobus acidocaldarius) with those of four representative AOB (Nitrosospira briensis, Nitrosomonas europaea, Nitrosolobus multiformis, and Nitrosococcus oceani) using potentiometric and calorimetric titrations to evaluate differences in proton-ionizable surface sites. Strain SCM1 and S. acidocaldarius have a wider range of proton buffering (approximately pH 10-3.5) than the AOB (approximately pH 10-4), under the conditions investigated. Thermodynamic parameters describing proton-ionizable sites (acidity constants, enthalpies, and entropies of protonation) are consistent with these archaea having proton-ionizable amino acid side chains containing carboxyl, imidazole, thiol, hydroxyl, and amine functional groups. Phosphorous-bearing acidic functional groups, which might also be present, could be masked by imidazole and thiol functional groups. Parameters for the AOB are consistent with surface structures containing anionic oxygen ligands (carboxyl- and phosphorous-bearing acidic functional groups), thiols, and amines. In addition, our results showed that strain SCM1 has more reactive surface sites than the AOB and a high concentration of sites consistent with aspartic and/or glutamic acid. Because these alternative boundary layers mediate interaction with the local external environment, these data provide the basis for further comparisons of the thermodynamic behavior of surface reactivity toward essential nutrients.

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“…A plethora of techniques have been used in studying metal adsorption onto bacteria including: calorimetry (Gorman-Lewis et al, 2006;Gorman-Lewis, 2009; Harrold and Gorman-Lewis, 2013), bulk adsorption experiments (Churchill et al, 1995;Fein et al, 1997;Ledin et al, 1997;Yee and Fein, 2001), X-ray photo-electron spectroscopy (XPS) (Kushwaha et al, 2012;Chubar et al, 2013), Fourier transform infrared spectroscopy (FTIR) (Jiang et al, 2004;Yee et al, 2004;Fang et al, 2011), molecular dynamics simulations (Johnson et al, 2006;Lins et al, 2008;Barkleit et al, 2009), and X-ray absorption spectroscopy (XAS) (Kelly et al, 2001;Boonfueng et al, 2009;Dunham-Cheatham et al, 2011). The first studies of the binding sites of metals on bacterial cell walls quantified adsorption under high metal loading conditions and identified carboxyl, phosphoryl, and amino moieties as the dominant groups responsible for metal binding (Beveridge andMurray, 1976, 1980;Guiné et al, 2006;González et al, 2014).…”

Section: Introductionmentioning

confidence: 99%