Calycosin-7-O-β-D-glucoside Attenuates OGD/R-Induced Damage by Preventing Oxidative Stress and Neuronal Apoptosis via the SIRT1/FOXO1/PGC-1α Pathway in HT22 Cells

Abstract

Neuronal apoptosis induced by oxidative stress is a major pathological process that occurs after cerebral ischemia-reperfusion. Calycosin-7-O-β-D-glucoside (CG) is a representative component of isoflavones in Radix Astragali (RA). Previous studies have shown that CG has potential neuroprotective effects. However, whether CG alleviates neuronal apoptosis through antioxidant stress after ischemia-reperfusion remains unknown. To investigate the positive effects of CG on oxidative stress and apoptosis of neurons, we simulated the ischemia-reperfusion process in vitro using an immortalized hippocampal neuron cell line (HT22) and oxygen-glucose deprivation/reperfusion (OGD/R) model. CG significantly improved cell viability and reduced oxidative stress and neuronal apoptosis. In addition, CG treatment upregulated the expression of SIRT1, FOXO1, PGC-1α, and Bcl-2 and downregulated the expression of Bax. In summary, our findings indicate that CG alleviates OGD/R-induced damage via the SIRT1/FOXO1/PGC-1α signaling pathway. Thus, CG maybe a promising therapeutic candidate for brain injury associated with ischemic stroke.

Exploring the Oxidative Stress Mechanism of Buyang Huanwu Decoction in Intervention of Vascular Dementia Based on Systems Biology Strategy

“…Studies have found that its specific anti-inflammatory mechanism in the treatment of neurodegenerative diseases is related to the TLR/NF- κ B and MAPK pathways [ 45 ]. In an in vitro model of cerebral ischemia and reperfusion, calycosin can prevent oxidative stress and neuronal apoptosis of HT22 cells through the SIRT1/FOXO1/PGC-1 α pathway, thereby reducing OGD/R-induced damage [ 46 ]. As the main active substance of Hedysarum multijugum Maxim ., astragaloside IV has a variety of pharmacological effects, including anti-inflammatory, antifibrosis, antioxidative stress, antiasthma, antidiabetic, and immune regulation [ 47 ].…”

Calycosin-7-O-β-D-Glucoside Treatment Promotes Axonal Regeneration via Rho/ROCK Pathway After Ischemia/Reperfusion Injury

“…, as a medicinal ingredient of AR. Our previous researches had confirmed that CG can reduce oxidative stress and neuronal apoptosis [12], and improved expression of GAP43 in PC12 cell oxygen-glucose deprivation/reperfusion (OGD/R) model, suggesting that CG can promote axonal regeneration. [13].Nevertheless, how CG exert protection for I/R injury is unclear.…”

Ginkgolide B Maintains Calcium Homeostasis in Hypoxic Hippocampal Neurons by Inhibiting Calcium Influx and Intracellular Calcium Release

“…A hypoxic environment can severely damage organs, especially the brain. Numerous investigations have provided evidence that a hypoxic environment can induce oxidative stress (Ramanathan et al, 2005 ; Niki, 2010 ; Yan et al, 2019 ), apoptosis (Li et al, 2007 ; Leszczynska et al, 2015 ), inflammation (Yang et al, 2013 , 2015 ; Koh et al, 2015 ), autophagy (Xu and Zhang, 2011 ; Yang et al, 2015 ) and Ca 2+ overload (Zhao et al, 1999 ) among other deleterious effects. Therefore, efforts are underway to develop new strategies to attenuate or prevent the damage caused by hypoxia, and there has been recent interest in the use of alternative medicines to antagonize hypoxia-induced tissue damage, such as aminophylline (Yang et al, 2007 ) diazepam, dexamethasone (Gong et al, 2015 ), choline (Zhang et al, 2017 ) and Rhododendron simsii Planch flower (Guo et al, 2020 ).…”