Camellia euphlebia flower extract inhibits oleic acid-induced lipid accumulation via reduction of lipogenesis in HepG2 cells

He, Dongye; Zhang, Ping; Sai, Xuan; Li, Xiaoyu; Wang, Lili; Xu, Yongping

doi:10.1016/j.eujim.2017.11.002

Cited by 17 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intracellular mechanism of lipid metabolism involves multiple pathways, such as the AMP‐activated protein kinase, PPAR, and Srebp signaling pathways (He et al., 2018). Gene expression associated with lipid metabolism further supported the changes induced by ESA.…”

Section: Resultsmentioning

confidence: 99%

Effects of epoxy stearic acid on lipid metabolism in HepG2 cells

Liu

2020

Journal of Food Science

View full text Add to dashboard Cite

In the present study, effects of cis-9,10-epoxystearic acid (ESA) generated by the thermal oxidation of oleic acid on HepG2 cells, including intracellular lipid accumulation, fatty acid composition, and lipid metabolism, were investigated. Our results revealed that ESA increased the number and size of cellular lipid droplets. Intracellular triacylglycerol and total cholesterol content demonstrated that ESA induced lipid accumulation in HepG2 cells in a dose-and time-dependent manner. Results of fatty acid composition further indicated that ESA could lead to intracellular lipid accumulation. Our results also revealed that ESA may suppress the fatty acid oxidation in peroxisomes and mitochondria, including PPARα, Cpt1α, and Acox1, whereas the expression of genes involved in lipid synthesis, including Srebp-1c and Scd1, was enhanced. These findings provide critical information on the effects of ESA on HepG2 cells, particularly lipid accumulation and metabolism, which is important for evaluating the biosafety of the oxidative product of oleic acid.

show abstract

Section: Resultsmentioning

confidence: 99%

Effects of epoxy stearic acid on lipid metabolism in HepG2 cells

Liu

2020

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…HepG2 cells have been widely used to investigate the mechanism of metabolic diseases including diabetes and NAFLD (Fan et al., 2019; He et al., 2018). In obese individuals, elevated free fatty acids (FFAs) have been identified as a critical pathogenic factor of NAFLD and diabetes (Karpe et al., 2011).…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the insulin‐resistant HepG2 cell model was established by treating high concentration insulin (10 −6 mol/L) for 24 hr, moreover, NAFLD model was induced by oleic acid in order to investigate the therapeutic benefit of constituents from H. patulum and characterize the underlying mechanism of action. According to relevant literature (He et al., 2018), the optimal concentration was selected from three concentrations of oleic acid (100, 150, 200 μM) to establish the model of NAFLD in HepG2 cells. It was concluded that HepG2 cells treated with 150 μM OA for 24 hr was the best choice to establish the NAFLD model.…”

Section: Discussionmentioning

confidence: 99%

“…Among these compounds, compounds 1 , 2 , 3 , 5 , 6 , 9 , 10, and 11 were first isolated from this plant. A considerable amount of literature has reported that flavonoids and their derivatives could enhances glucose uptake in insulin resistant HepG2 cells and inhibit lipid accumulation in oleic acid‐induced HepG2 cells via different pathway (He et al., 2018; Janda et al., 2016; Jin et al., 2013; Mokashi et al., 2017). Phenylpropanoids have also been reported to show promising insulin resistance and glucose metabolism activity (Saadeldeen et al., 2020).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Flavonoids from Hypericum patulum enhance glucose consumption and attenuate lipid accumulation in HepG2 cells

et al. 2021

View full text Add to dashboard Cite

Hypericum patulum has been used as a folk medicine for its varied therapeutic effects including antifungal, wound‐healing, spasmolytic, stimulant, hypotensive activities. The water decoction is drank as tea could treat cold, infantile malnutrition. The present study aims to isolate the constituents of the plant and investigate their effects on the glucose consumption in insulin‐resistant HepG2 cells, furthermore, lipid metabolism in oleic acid (OA)‐treated HepG2 cells was also studied. The phytochemical investigation of the plant led to the isolation of eleven compounds, and their structures were identified by spectroscopic analysis as n‐dotriacontanol (1), shikimic acid (2), 1‐O‐caffeoylquinic acid methyl ester (3), 5‐O‐caffeoylquinic acid methyl ester (4), 5‐O‐coumaroylquinic acid methyl ester (5), 5‐O‐caffeoylquinic acid butyl ester (6), quercetin‐3‐O‐α‐L‐rhamnoside (7), quercetin (8), quercetin‐3‐O‐(4״‐methoxy)‐α‐L‐rahmnopyranosyl (9), hyperoside (10), and rutin (11). The results revealed that compounds 7, 9, and 10 could enhance glucose consumption significantly in hyperglycemia induced HepG2 cells and insulin‐resistant HepG2 cells. In addition, the western blotting analysis result exhibited that compounds 7, 9, and 10 in high concentration (5 μM, H) group could dramatically upregulate the expression of PPARγ protein, and even the effect of them had no significant difference compared with that of rosiglitazone. Furthermore, compounds 9 and 10 in middle concentration (2.5 μM, M) group and H group could dramatically promote triglyceride metabolism and decrease TG content in OA‐treated HepG2 cells, and even in H group, reactive oxygen species (ROS) level were significantly decreased compared with model group. Practical applications Hypericum patulum is a well‐known plant of the genera Hypericum for its varied preventive and therapeutic potential activities. To study the chemical constituents and their effects on glucose and lipid metabolism in vitro, we detected glucose consumption in insulin‐resistant HepG2 cells, triglyceride content and reactive oxygen species level in OA‐treated HepG2 cells. In addition, PPARγ protein was also detected by western blotting analysis in the study. Compounds 1, 2, 3, 5, 6, 9, 10, and 11 were isolated from the plant for the first time. Quercetin‐3‐O‐(4"‐methoxy)‐α‐L‐rahmnopyranosyl (9) and hyperoside (10) had potential therapeutic benefit against glucose and lipid metabolic disease. Therefore, this study might have certain guiding significance for further research and development of H. patulum.

show abstract

“…Triglyceride accumulation in hepatocyte cytoplasm is the hallmark of hepatic steatosis, and pathology examinations have demonstrated that patients with NAFLD exhibit a high level of free fatty acid in their blood serum. Oleic acid (OA), a typical free fatty acid in hepatic triglyceride, has been utilized to establish in vitro cellular models to investigate lipid uptake, triglyceride synthesis, and fatty acid oxidation in the process of hepatic steatosis (He et al, 2018;Kuo et al, 2012;Rafiei et al, 2019). These cell models reemerge the symptoms of hepatic steatosis in patients, assisting researchers in finding potential intervention drugs and therapeutic schedules (Xie et al, 2016).…”

Section: Introductionmentioning

confidence: 99%