Campomanesia adamantium (Myrtaceae) fruits protect HEPG2 cells against carbon tetrachloride-induced toxicity

Abstract

Campomanesia adamantium (Myrtaceae) is an antioxidant compounds-rich Brazilian fruit popularly known as gabiroba. In view of this, it was evaluated the hepatoprotective effects of pulp (GPE) or peel/seed (GPSE) hydroalcoholic extracts of gabiroba on injured liver-derived HepG2 cells by CCl4 (4 mM). The results showed the presence of total phenolic in GPSE was (60%) higher when compared to GPE, associated with interesting antioxidant activity using DPPH• assay. Additionally, HPLC chromatograms and thin layer chromatography of GPE and GPSE showed the presence of flavonoids. Pretreatment of HepG2 cells with GPE or GPSE (both at 800–1000 μg/mL) significantly (p < 0.0001) protected against cytotoxicity induced by CCl4. Additionally, the cells treated with both extracts (both at 1000 μg/mL) showed normal morphology (general and nuclear) contrasting with apoptotic characteristics in the cells only exposed to CCl4. In these experiments, GPSE also was more effective than GPE. In addition, CCl4 induced a marked increase in AST (p < 0.05) and ALT (p < 0.0001) levels, while GPE or GPSE significantly (p < 0.0001) reduced these levels, reaching values found in the control group. In conclusion, the results suggest that gabiroba fruits exert hepatoprotective effects on HepG2 cells against the CCl4-induced toxicity, probably, at least in part, associated with the presence of antioxidant compounds, especially flavonoids.

Assessment of Cytotoxic Activity of Rosemary (Rosmarinus officinalisL.), Turmeric (Curcuma longaL.), and Ginger (Zingiber officinaleR.) Essential Oils in Cervical Cancer Cells (HeLa)

“…Each cell line was cultured in complete Dulbecco's Modified Eagle's Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA), supplemented with 20% (v/v) fetal bovine serum (FBS) (GIBCO-Invitrogen, Indianapolis, IN, USA), solution containing penicillin (100 IU/mL) and streptomycin (100  μ g/mL) (Sigma-Aldrich, St. Louis, MO, USA), and 2  μ g/mL amphotericin B (Sigma-Aldrich, St. Louis, MO, USA). The cells were kept in an incubator (Panasonic®, Chicago, IL, USA) in a humidified atmosphere of 5% CO 2 in air at 37°C [14, 15]. …”

Investigation of Campomanesia Components: A Fruit of Brazilian Cerrado

“…C. adamantium (Myrtaceae) is an antioxidant fruit, and results showed the hepatoprotective effects of pulp or peel/seed hydroalcoholic extracts on injured liverderived HepG2 cells by CCl4. The results in part are associated with the presence of antioxidant compounds, especially flavonoids [16]. Researches and studies prove that the oleic and linoleic fatty acids obtained from the oil of the seeds from C. xanthocarpa Berg.…”

Campomanesia sp. flour attenuates non‐alcoholic fatty liver disease on rats fed with a hypercaloric diet

“…The fruit pulp has greater technological applicability than any other part. Its peel and seeds are considered industrial residue; on the other hand, studies have shown that this sample has great effectiveness antioxidant protection in cells, thanks to its bioactive compounds (Alves, Alves, Fernandes, Naves, & Naves, 2013; de Oliveira Fernandes, Ávila, Moura, Ribeiro, Naves, & Valadares, 2015). No evidence of toxicity was found in the fruit peel, so it is considered safe to be part of the human diet.…”