Campomanesia velutina leaves extracts exert hypouricemic effects through inhibition of xanthine oxidase and ameliorate inflammatory response triggered by MSU crystals

Araújo, Marcela C.P.M.; Ferraz-Filha, Zilma Schimith; Ferrari, Fernanda Cristina; Saúde-Guimarães, Dênia Antunes

doi:10.1016/j.bjp.2016.05.016

Cited by 14 publications

(6 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The blood is enabled to clot for around 1 h at room temperature before being centrifuged for 10 min at 2500 rpm (revolutions per minute). The serum is isolated and deposited at 20 °C before the uric acid assay is performed [ 26 ].…”

Section: Experimental In-vivo Models For Goutmentioning

confidence: 99%

See 1 more Smart Citation

A brief review on in vivo models for Gouty Arthritis

Patil¹,

Soni²,

Acharya³

2021

Metabolism Open

View full text Add to dashboard Cite

Gout is more common in men than in women, by a factor of 3.1–10.1. Gout prevalence and incidence have increased in recent decades, with prevalence reaching 11–13% and incidence reaching 0.4% in people over the age of 80. Age-related renal impairment, altered drug distribution, and increased prevalence of comorbidities have significant consequences for safe and effective gout pharmacotherapy. The Discovery of Fruitful in-vivo animal models needs the effective screening of drugs or formulations used in the treatment of gout. In vivo animal models of Gouty arthritis are extensively used to investigate pathogenic mechanisms governing inflammation-driven bone and cartilage damage. Four commonly utilized models include the Potassium oxonate induced hyperuricemic model, MSU crystals induced gouty arthritis animal model, Animal Model of Acute Gouty Arthritis with Hyperuricemia, and Diet-induced hyperuricemia. These offer unique advantages for correlating different aspects of gouty arthritis with human disease. In-vivo animal models served as testing beds for novel biological therapies, including cytokine blockers and small molecule inhibitors of intracellular signaling that have revolutionized gouty arthritis treatment. This review highlights a brief overview of in vivo experimental models for assessment of hypouricemic, anti-inflammatory, as well as renal protective effects of test compounds with some evaluation parameters in detail.

show abstract

Section: Experimental In-vivo Models For Goutmentioning

confidence: 99%

“…After systematically removing the lipid layer, the supernatant would be centrifuged at 10,000 rpm for 60 min at 4 °C. Enzyme assays would be performed on the final supernatant [ 26 ]. Procedure: …”

Section: Evaluation Parameters For In-vivo Modelsmentioning

confidence: 99%

A brief review on in vivo models for Gouty Arthritis

Patil¹,

Soni²,

Acharya³

2021

Metabolism Open

View full text Add to dashboard Cite

show abstract

“…Xanthine oxidase (XO) is a pivotal enzyme involved in the conversion of hypoxanthine to xanthine and then xanthine to uric acid as well as oxygen species (free radicals). If XO is highly expressed, which results in overproduction of uric acid (hyperuricemia) and precipitate as monosodium urate (MSU) and subsequently deposit in the articular joints (especially knees) via triggering inflammation and eventually end up in gout (Araújo, Ferraz‐Filha, Ferrari, & Saúde‐Guimarães, ). Moreover, hyperuricemia is highly associated with various diseases like metabolic syndrome, pulmonary and renal disorders owing to excessive free radical generation (Hayden & Tyagi, ; Puig & Martinez, ).…”

Section: Introductionmentioning

confidence: 99%

Anti-mutagenicity, hypouricemic and antioxidant activities of alkaloids from vinegar and mei vinegar

Chiu

Cheng

et al. 2017

J Food Biochem

View full text Add to dashboard Cite

The objective of the present experiment was to assess the protective activity of sorghum vinegar (V) and mei vinegar (MV) and its alkaloids by evaluating its antioxidant, anti‐mutagenic, and hypouricemic activities. The acetic acid concentration, metallic ions like calcium (Ca), copper (Cu), iron (Fe), sodium (Na), potassium (K), zinc (Zn), magnesium (Mg), manganese (Mn), and alkaloid contents (β‐carbolines) were much higher in MV than V. MV exhibited better xanthine oxidase (XO) inhibitory activity to endorse its antihyperuricemic effect. Moreover, the antioxidant capacity and solubility of urate were substantially ameliorated in MV than V. Both V and MV effectively reduced the mutagenicity induced by mutagenic agents like N‐methyl‐N′‐nitro‐N‐nitroso‐guanidine, tert‐butyl hydroperoxide, and 2‐amino‐3‐methylimidazo‐[4,5‐f]‐quinoline on various strains of Salmonella typhimurium namely TA100 and TA102. Based on the outcome of the current study, it indicated that MV might be recommended for treating the uric acid related disorder (gout) along with standard hypouricemic drugs. Practical applications For many year mei vinegar (plum) has been used as a physiological alkaline food and hence recommended for treating various ailments like an ulcer, gastritis, and arthritis (gout). However, the scientific evidence behind vinegar for treating arthritis (gout) was unknown and hence the current study was undertaken to showcase the beneficial activity by checking the antioxidant, antimutagenic and hypouricemic activities of MV and V. Ample amount of studies proved that alkaloids (β‐carboline) are the major phytocomponents of fruit or grain vinegar with numerous health promoting properties and hence, we speculate that alkaloids in MV might largely contribute to antioxidant, antimutagenic, and hypouricemic activities. Our results confirm our hypothesis and indicating that MV with higher content of alkaloids showed better beneficial property than V. Therefore, MV can be used as a functional food for curbing uric acid related disorder.

show abstract

“…Nos extratos, aquoso e etanólico, das folhas da C. velutina, foram encontrados dois flavonoides prevalentes a rutina e miricetrina (Araújo et al, 2016), os quais possuem relatos em várias atividades biológicas. Estudos recentes encontraram concentrações maiores de flavonoides totais (2507 µg de equivalentes de Rutina/g de extrato) em extratos aquosos de folhas da Campomanesia xanthocarpa em comparação ao presente trabalho, levando a crer que o método extrativo e tipo de solvente influenciem consideravelmente na concentração de compostos bioativos da mesma espécie de planta (Sant'Anna et al, 2017).…”

Section: Resultsunclassified