Campylobacter upsaliensis, C. sputorum sputorum and C. concisus as Common Causes of Diarrhoea in Swedish Children

Lindblom, Gun-Britt; Sjögren, Eva; Hansson-Westerberg, Jane; Kaijser, B

doi:10.3109/00365549509019006

Cited by 86 publications

(68 citation statements)

References 4 publications

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“…Taken together, these findings suggest that a pathogenic role exists for at least certain subtypes of C. concisus strains. The need to establish whether C. concisus genomospecies 2 is an emerging human pathogen is evident in light of its high isolation rates in selected hospital settings (1,10,15,18,30).…”

Section: Discussionmentioning

confidence: 99%

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Delineation of Campylobacter concisus Genomospecies by Amplified Fragment Length Polymorphism Analysis and Correlation of Results with Clinical Data

Aabenhus

Siemer

et al. 2005

J Clin Microbiol

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Campylobacter concisus has been as frequently isolated from human diarrhea as the important enteropathogen Campylobacter jejuni, but it also occurs in the feces of healthy individuals. The role of C. concisus in human disease has been difficult to determine, since the species comprises at least two phenotypically indistinguishable but genetically distinct taxa (i.e., genomospecies) that may vary in pathogenicity. We examined 62 C. concisus strains by amplified fragment length polymorphism (AFLP) profiling and correlated the results with clinical data. All C. concisus strains gave unique AFLP profiles, and numerical analysis of these data distributed the strains among four clusters. The clustering was of taxonomic significance: two clusters contained, respectively, the type strain (of oral origin) and a reference strain (from diarrhea) of each of the known genomospecies. Genomospecies 2 strains were more frequently isolated from immunocompetent patients and/or patients without concomitant infections that presented with fever, chronic diarrhea, and gut inflammation than was genomospecies 1, clustering with the type strain of oral origin. Bloody diarrhea was recorded only with C. concisus genomospecies 2 infections. We identified two additional C. concisus genomospecies: genomospecies 3 comprised a single strain from an immunocompetent patient, and genomospecies 4 contained five isolates from severely immunodeficient patients, i.e., organ transplantation recipients or those with hematological malignancies. All genomospecies 4 strains were of the same protein profile group and failed to react with a C. concisus species-specific PCR assay based on 23S rRNA gene sequences: the taxonomic position of this group requires closer investigation. Campylobacter concisus is genetically and taxonomically diverse and contains at least four distinct genomospecies that may exhibit differences in their spectra of virulence potential.

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Section: Discussionmentioning

confidence: 99%

“…However, it has also been detected in fecal samples from both healthy (9,10,30) and diarrhetic patients (1,9,10,15,18,20,22,29,30). As a consequence, its primary pathogenic potential is uncertain.…”

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confidence: 99%

Delineation of Campylobacter concisus Genomospecies by Amplified Fragment Length Polymorphism Analysis and Correlation of Results with Clinical Data

Aabenhus

Siemer

et al. 2005

J Clin Microbiol

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“…IBD is a chronic inflammation of the gastrointestinal tract, and the two major clinical forms are Crohn's disease (CD) and ulcerative colitis (UC). In addition to IBD, C. concisus has been associated with diarrhoeal disease; several studies have reported the isolation of C. concisus from diarrhoeal faecal samples (Lastovica, 2006;Lindblom et al, 1995;Nielsen et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Delineation of genetic relatedness and population structure of oral and enteric Campylobacter concisus strains by analysis of housekeeping genes

et al. 2015

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Campylobacter concisus is an oral bacterium that has been shown to be associated with inflammatory bowel disease (IBD). In this study we examined clusters of oral C. concisus strains isolated from patients with IBD and healthy controls by analysing six housekeeping genes. In addition, we investigated the population structure of C. concisus strains. Whether oral and enteric strains form distinct clusters based on the sequences of these housekeeping genes was also investigated. The oral C. concisus strains were found to contain two genomospecies, which belong to the two genomospecies previously found in enteric C. concisus strains. C. concisus clusters formed based on the sequences of a single aspA gene were the same as that formed by using previously reported MLST schemes. The analysis of combined oral and enteric C. concisus strains found that enteric C. concisus strains did not form distinct clusters. Genetic structure analysis identified five subpopulations of C. concisus and showed that genetic recombination between C. concisus strains was common. However, genetic recombination was significantly less in oral strains isolated from patients with IBD than from healthy individuals. Previously reported oral and enteric intestinal epithelial invasive C. concisus strains were in cluster II and subpopulation III. Furthermore, this study shows that there are no distinct enteric C. concisus strain clusters or subpopulations.

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“…concisus strains were isolated from children with diarrhoea in different countries (Russell, 1995;Lindblom et al, 1995;le Roux & Lastovica, 1998), and are known to be most common in some patient subgroups, mainly children under 24 months of age and immunocompromised patients (Engberg et al, 2000;Lastovica & le Roux, 2000;Aabenhus et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a haemolytic phospholipase A2 activity in clinical isolates of Campylobacter concisus

Istivan

Coloe

Fry

et al. 2004

Journal of Medical Microbiology

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A membrane-bound, haemolytic phospholipase A 2 (PLA 2 ) activity was detected in clinical strains of Campylobacter concisus isolated from children with gastroenteritis. The clinical strains were assigned into two molecular groups (genomospecies) based on PCR amplification of their 23S rDNA. This calcium-dependent, heat-stable, haemolytic PLA 2 activity was detected in strains from both genomospecies. A crude haemolysin extract (CHE) was initially prepared from cellular outermembrane proteins of these isolates and was further fractionated by ultrafiltration. The haemolytic activity of the extracted fraction (R30) was retained by ultrafiltration using a 30 kDa molecular mass cut-off filter, and was designated haemolysin extract (HE). Both CHE and HE had PLA 2 activity and caused stable vacuolating and cytolytic effects on Chinese hamster ovary cells in tissue culture. Primers for the conserved region of pldA gene (phospholipase A gene) from Campylobacter coli amplified a gene region of 460 bp in all tested isolates, confirming the presence of a homologous PLA gene sequence in C. concisus. The detection of haemolytic PLA 2 activity in C. concisus indicates the presence of a potential virulence factor in this species and supports the hypothesis that C. concisus is a possible opportunistic pathogen.

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Campylobacter upsaliensis, C. sputorum sputorum and C. concisus as Common Causes of Diarrhoea in Swedish Children

Abstract: Scand J Infect Dis Downloaded from informahealthcare.com by Kungliga Tekniska Hogskolan on 08/10/15For personal use only.

Cited by 86 publications

References 4 publications

Delineation of Campylobacter concisus Genomospecies by Amplified Fragment Length Polymorphism Analysis and Correlation of Results with Clinical Data

Delineation of Campylobacter concisus Genomospecies by Amplified Fragment Length Polymorphism Analysis and Correlation of Results with Clinical Data

Delineation of genetic relatedness and population structure of oral and enteric Campylobacter concisus strains by analysis of housekeeping genes

Characterization of a haemolytic phospholipase A2 activity in clinical isolates of Campylobacter concisus

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