Wounding of trees by debarking has been reported to form a callus tissue. This work aims to investigate macroscopic and microscopic modifications of wood after the removal of barks in Alstonia boonei, Scorodophloeus zenkeri, and Garcinia lucida. Species that are not yet barking were wounded to conducting experimental debarking. The wound was rectangular with 30 cm vertically with a lateral extent of 10 cm. Every three months, there was a follow-up for fifteen months during which the macroscopic and microscopic phenomena were observed and recorded. Microsections of 15–20 μm thickness were taken on a piece of wood from the regenerated and intact wood, with a sledge microtome. Staining of cell walls was done with safranin and fast green to increase contrast in an anatomical slide. Analyzes of the variability of xylem anatomical features were done by semi-automatic measurements using the SpectrumSee digital image analysis software. The speed of recovery of the bark of these three species is 9.04 cm/year for A. boonei, 5.9 cm/year for S. zenkeri, and 3.85 cm/year for G. lucida. The recovery of A. boonei’s bark is the fastest, and it just takes 15 months to heal its wound. Densities of vessels were 8, 38, and 17 per mm², respectively, for the wood of A. boonei, S. zenkeri, and G. lucida before barking. These values increased for A. boonei (26) and G. lucida (20) except for S. zenkeri (25). In all species, the diameter of the vessels has decreased in the regenerated wood. Management practices that enhance the monitoring of sustainable harvesting levels of species and promote alternative plants for the same uses should be considered as part of conservation strategies.