Can elevated prolidase activity predict the duration of ischemic exposure in different types of ischemia?

Abstract

Bu çalışmada farklı iskemi tiplerinde serum prolidaz aktivitesi ve iskemi süresi arasındaki ilişkinin belirlenmesi amaçlandı. Ça lış ma pla nı: Kırk erkek Sprague-Dawley cinsi sıçan beş eşit gruba bölündü. Grup 1'deki sıçanlar (kontrol grubu) bazal serum prolidaz düzeylerinin belirlenmesi için herhangi bir işlem uygulanmadan sakrifiye edildi ve kan örnekleri alındı. Grup 2 ve 3'de mezenterik iskemi oluşturmak için basit laparotomi yapılarak, superior mezenterik arterler klemplendi. Grup 4 ve 5'de periferik iskemi oluşturmak için sağ ana femoral arter klemplenerek sırasıyla 120. ve 360. dakikalarda kan örnekleri alındı. Tüm gruplardan elde edilen örneklerden serum prolidaz düzeyi ölçüldü. Bul gu lar: Sıçanlardaki bazal prolidaz düzeyleri 266.8±20.5 U/L olarak bulundu. Periferik (404.0±105.6 U/L) ve mezenterik iskeminin (317.1±121.4 U/L) iki saat sonrasında serum prolidaz aktiviteleri arttı. Ancak, periferik (346.1±104.9 U/L) ve mezenterik iskeminin (233.4±36.6 U/L) altı saat sonrasında serum prolidaz düzeyleri azaldı. Serum prolidaz düzeyleri mezenterik iskemide artmasında rağmen, bu periferik iskemide iki saat sonra elde edilen enzim düzeylerinden daha düşüktü (p=0.006). So nuç: Serum prolidaz aktivitesi, iskemi süresinin belirlenmesinde önemli bir öngördürücü biyobelirteç olabilir. Anah tar söz cük ler: Biyobelirteç; iskemi süresi; mezenterik iskemi; periferik iskemi; prolidaz düzeyi. Background:This study aims to determine the relationship between serum prolidase activity and ischemia duration in different ischemia types. Methods: Forty male Sprague Dawley rats were divided into five equal groups. The rats were sacrificed and blood samples were obtained to determine the basal serum prolidase levels in group 1 (control group) without any intervention. In groups 2 and 3, the superior mesenteric arteries were clamped with simple laparotomy to induce mesenteric ischemia. In groups 4 and 5, the right common femoral artery was clamped to induce peripheral ischemia and blood samples were taken at 120 and at 360 minutes, respectively. The serum prolidase levels were measured using the samples obtained from each group. Results: The basal prolidase level in rats was found to be 266.8±20.5 U/L. The serum prolidase levels increased after two-hours of peripheral (404.0±105.6 U/L) and mesenteric ischemia (317.1±121.4 U/L). However, the serum prolidase levels decreased after six-hours of peripheral (346.1±104.9 U/L) and mesenteric ischemia (233.4±36.6 U/L). Although the serum prolidase levels were elevated in the second hour of mesenteric ischemia, they were lower than the enzyme levels obtained after twohours of peripheral ischemia (p= 0.006). Conclusion: The serum prolidase level may be an important predictive biomarker for identifying the duration of ischemia.

Comparison of Renoprotective Effect of Dabigatran With Low-Molecular-Weight Heparin

“…In particular, previous studies in the literature have claimed that renal tissues have the highest prolidase activity in the human body. 15 Previous studies also reported increased serum prolidase activities in degenerative valve disease and lung fibrosis due to the increased collagen turnover. 16,17 Significantly elevated MDA levels have been reported in previous I/R studies.…”

Association of Serum Omentin-1 Concentrations with the Presence of Atrial Fibrillation

“…These results point to an important role for adipose tissue and adipokine in the pathophysiology of AF. Nowadays, serum biomarkers are used in the diagnosis and prediction of cardiovascular disease [12,13]. Therefore, serum omentin-1 may be utilized as a new biomarker to predict and assess the risk of developing AF.…”

Protective effects of ginseng extracts and common anti-aggregant drugs on ischaemia-reperfusion injury : cardiovascular topics

“…Prolidase is a marker for collagen metabolism that is related to increased levels of nuclear hypoxia-inducible factor-1 alpha (HIF-1). 9 , 27 Increased prolidase levels were reported in acute ischaemic events. 27 …”