Can extra‐embryonic celomic fluid be partially replaced with stem‐cell culture medium?

Santolaya-Forgas, Joaquín; DeLeon-Luis, J.; Galán, Isabel

doi:10.1002/uog.2848

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…The presence of such precursors in CF may suggest cellular trafficking between the embryo and the CC. The possibility of early access to the CC in combination with cell trafficking between embryonal compartments could open a novel strategy for in utero cell transplantation . Megaloblasts and cells with pseudopodia are the main cellular components used in our center for prenatal diagnosis by celocentesis because they are frequently present and easily identifiable because of their morphology.…”

Section: Discussionmentioning

confidence: 99%

“…The possibility of early access to the CC in combination with cell trafficking between embryonal compartments could open a novel strategy for in utero cell transplantation. [32][33][34] Megaloblasts and cells with pseudopodia are the main cellular components used in our center for prenatal diagnosis by celocentesis because they are frequently present and easily identifiable because of their morphology. The robustness and reproducibility of this methodology to classify these cells in relation to their morphology were demonstrated by the correct association of their morphology to their origin in all pooled cells analyzed.…”

Section: Discussionmentioning

confidence: 99%

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Identification of embryo–fetal cells in celomic fluid using morphological and short‐tandem repeats analysis

Giambona¹,

Leto²,

Damiani

et al. 2016

Prenatal Diagnosis

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of embryo–fetal cells in celomic fluid using morphological and short‐tandem repeats analysis

Giambona¹,

Leto²,

Damiani

et al. 2016

Prenatal Diagnosis

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“…The authors have also reported on (1) some of the biological roles of celomic fluid during early development, 31,32 (2) the feasibility of prenatal diagnosis using celomic fluid, 33 (3) the survival to term after injection of human umbilical cord stem cells via celocentesis at 40 days from fertilization (baboon animal model term $ 180 days), 34 and (4) the fact that baboon celomic fluid can be partially replaced in vivo by stem cell culture medium without causing abortion or any obvious congenital malformations. 35 Although this last study was performed in three animals, it is important because the celomic fluid could potentially serve as a bioreactor for prolonging the survival and expansion of a limited number of stem cells injected into the celomic cavity. However, despite these reports, the therapeutic application of stem cell transplantation via celocentesis in primates remains to be determined.…”

Section: Is the Timed-pregnant Baboon Animal Model An Appropriate Surmentioning

confidence: 99%

Celocentesis for In Utero Stem Cell Therapy: Where We Now Stand and Future Directions

2007

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As the number of inborn errors of metabolism that can be diagnosed prenatally by direct DNA analysis increases, so will the number of patients interested in having prenatal diagnosis for these conditions. The phenotypic expression of these inherited inborn errors of metabolism in theory could be prevented by the in utero transplantation of donor stem cells. It is the authors' opinion that ultrasound-guided celocentesis performed in the timed-pregnant baboon animal model could have an important role for determining if stem cell transplantation in the nonfunctional immunocompetent embryo has any clinical potential.

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Acute changes in the embryonic heart rate: a response to environmental challenges?

Santolaya

Stefano

Luis

et al. 2012

Ultrasound in Obstet & Gyne

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Ultrasound detection of hyaloid artery in the third trimester of pregnancy: a pathological findingThe hyaloid artery is part of the embryonic vasculature of the eye and normally regresses during the second trimester of pregnancy 1 . Failure of regression of the hyaloid artery is a sign of abnormal eye development and is usually associated with a persistent hyperplastic primary vitreous (PHPV) 2 . The hyaloid artery disappears between 23 and 28 weeks, and is normally not visible on ultrasound after 29 weeks of gestation 3 . One report has linked the observation of cataracts and thickened hyaloid artery-lens junction on ultrasound at 23 weeks with the diagnosis of PHPV 4 . Persistence of the hyaloid artery on ultrasound examination during the third trimester is a rare pathological finding. We report a case of persistence of the hyaloid artery in the third trimester of pregnancy associated with other anomalies.A 31-year-old woman, gravida 2 para 1, was referred to our prenatal diagnosis center at 27 weeks' gestation for intrauterine growth restriction (IUGR). Her obstetric history consisted of one normal pregnancy with term delivery. The parents were not consanguineous. The firsttrimester screening ultrasound scan had found a normal nuchal translucency measurement, but maternal serum markers had not been obtained. Follow-up ultrasound confirmed IUGR at 30 weeks, with all fetometric measurements below the 3 rd centile but a normal umbilical Doppler. Ultrasound examination also showed hyperechogenic kidneys and a persistent hyaloid artery visible on a transverse view of the right fetal orbit (Figure 1a). Amniocentesis was performed revealing a normal karyotype, and no deletion of the chromosomal region 4p was found on fluorescence in-situ hybridization (FISH) analysis. Polymerase chain reaction (PCR) analysis of amniotic fluid was negative for cytomegalovirus. Fetal skeletal three-dimensional helical computer tomography and cerebral magnetic resonance imaging were performed and were found to be normal. The hyaloid artery was still present on ultrasound examination at 32 weeks. The morphological abnormalities observed, the uncertain prognosis and the suspicion of syndromic association were explained to the couple by a multidisciplinary team involving geneticists and pediatric ophthalmologists. In accordance with French legislation, termination of pregnancy was performed at the request of the parents. The woman delivered a 1175-g (< 3 rd centile) male baby at 34 weeks.Postmortem examination confirmed IUGR and revealed renal microcysts. Macroscopic and microscopic examination of the eyes also confirmed the presence of the right hyaloid artery (Figure 1b). Hyperplastic primary vitreous was not visible on histological examination, but lens histology revealed a developing cataract (Figure 2).Despite the histological absence of PHPV in our case, the persistence of the hyaloid artery belongs to the same spectrum of ocular malformations and our histological examination showed a unilateral right cataract, which

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Can extra‐embryonic celomic fluid be partially replaced with stem‐cell culture medium?

Cited by 5 publications

References 9 publications

Identification of embryo–fetal cells in celomic fluid using morphological and short‐tandem repeats analysis

Identification of embryo–fetal cells in celomic fluid using morphological and short‐tandem repeats analysis

Celocentesis for In Utero Stem Cell Therapy: Where We Now Stand and Future Directions

Acute changes in the embryonic heart rate: a response to environmental challenges?

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