Can fish oil supplementation and physical training improve oxidative metabolism in aged rat hearts?

Lopes, Andréia; Mendes-da-Silva, Rosângela Figueiredo; Nascimento, Luciano; Ferreira, Diorginis José Soares; Amâncio-dos-Santos, Ângela; Batista-de-Oliveira-Hornsby, Manuella; Lagranha, Cláudia Jacques

doi:10.1016/j.lfs.2015.07.021

Cited by 9 publications

(6 citation statements)

References 46 publications

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“…These findings are in agreement with other studies supporting, firstly, that age increases oxidative stress, and secondly that during aging, extra virgin olive oil treatment increases the activity of antioxidant enzymes in the liver [60]. Also, catalase activity in aged hearts of Wistar rats supplemented with fish oil has been reported to be higher compared with its control group [61]. Catalase and Se-GPX, involved in the removal of peroxides, it is well known that their activities increase with age [62].…”

Section: Discussionsupporting

confidence: 92%

Heart Histopathology and Mitochondrial Ultrastructure in Aged Rats Fed for 24 Months on Different Unsaturated Fats (Virgin Olive Oil, Sunflower Oil or Fish Oil) and Affected by Different Longevity

et al. 2019

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Diet plays a decisive role in heart physiology, with lipids having especial importance in pathology prevention and development. This study aimed to investigate how dietary lipids varying in lipid profile (virgin olive oil, sunflower oil or fish oil) affected the heart of rats during aging. Heart histopathology, mitochondrial morphometry, and oxidative status were assessed. Typical histopathological features associated with aging, such as valvular lesions, endomyocardical hyperplasia, or papillary muscle calcification, were found at a low extent in all the experimental groups. The most relevant finding was that inflammation registered by fish oil group was lower compared to the other treatments. At the ultrastructural level, heart mitochondrial area, perimeter, and aspect ratio were higher in fish oil-fed rats than in those fed on sunflower oil. Concerning oxidative stress markers, there were differences only in coenzyme Q levels and catalase activity, lower in sunflower oil-fed animals compared with those fed on fish oil. In summary, dietary intake for a long period on dietary fats with different fatty acids profile led to differences in some aspects associated with the aging process at the heart. Fish oil seems to be the fat most protective of heart during aging.

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Section: Discussionsupporting

confidence: 92%

Heart Histopathology and Mitochondrial Ultrastructure in Aged Rats Fed for 24 Months on Different Unsaturated Fats (Virgin Olive Oil, Sunflower Oil or Fish Oil) and Affected by Different Longevity

et al. 2019

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“…The capsules of fish oil (Sundown®) containing PUFAs [docosahexaenoic (DHA; 85 mg/L g) and eicosapentaenoic (EPA; 128 mg/1 g)] were dissolved in Cremophor (Sigma ®) 0.009% and distilled water. The FO group had the PUFAs solution administered via gavage, while the control group received a vehicle solution (same amount of Cremophor and distilled water used to prepare PUFAs solution) 45,46 .…”

Section: Fish Oil Supplementationmentioning

confidence: 99%

“…Firstly, animals were familiarized with the exercise apparatus for 5 days, then, an incremental running test was performed to provide the maximal running capacity of the animals throughout the training regimen. The physical exercise consisted of a 30 min continuous running on a treadmill (Insight EP-131, 0°inclination), 5 days/week, for 4 weeks, at 50% of the maximal running capacity, which was weekly assessed to readjust the training intensity 43,45 . The animals from sedentary groups were placed on the treadmill for the same period as the exercised animals, but the apparatus remained off.…”

Section: Treadmill Exercisementioning

confidence: 99%

Fish oil supplementation and physical exercise during the development period increase cardiac antioxidant capacity in Wistar rats

Melo,

Macêdo,

Costa

et al. 2022

Motriz: rev. educ. fis.

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To investigate if treadmill exercise (Ex) associated with fish oil (FO) supplementation during lactation would influence the biochemical profile as well as the oxidative balance in the hearts of male juvenile rats. Methods: Fifteen days-old rats were submitted to a daily moderate Ex training (based on their maximal running capacity) and FO supplementation for 4 weeks. Forty-eight hours after the last exercise session, blood fasting glucose and lipid profile were assessed according to the manufacturer's recommendations, while the oxidative status of the hearts was evaluated via colorimetric and absorbance-based assays. Results: FO associated with Ex decreased triglycerides (TG-79.27 ± 5.75 to 60.24 ± 6.25 mg/dL) and very-low-density lipoprotein cholesterol levels (VLDL-15.85 ± 1.15 to 12.05 ± 1.25 mg/dL) when compared to sedentary animals. FO, alone, reduced atherogenic index (AI-1.14 ± 0.03 vs. 1.01 ± 0.04 a.u) while increased high-density lipoprotein cholesterol (HDL-43.90 ± 2.50 vs. 59.43 ± 3.15 mg/dL) of sedentary animals. Additionally, both Ex (67.3 ± 13.5 nmol/mg prot) and FO supplementation (56.6 ± 5.5 nmol/mg prot) decreased the oxidative damage to lipids in non-trained animals (105.8 ± 10.8 nmol/mg prot). The interventions also protected the protein content from oxidative stress (Ex-5.15 ± 0.46; FO-4.5 ± 0.5; and vehicle sedentary-7.3 ± 0.6 µmol/mg prot), while increasing the antioxidant defense and oxidative metabolism. Conclusion: Our findings suggest that intervention in juvenile rats can improve cardiac metabolism. These are the first findings to show the positive effects of the association between FO and moderate treadmill Ex during the critical period of development. We believe these results can drive early-life origins of heart disease through different avenues and, possibly, assist the development of a heart disease prevention program as well as an adjunctive therapeutic resource.

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“…It is also known that the association of (Fo) supplementation and (Ex) are characterized by enhanced antioxidant defenses (da Silva Pedroza et al 2015). However, data show that the effects of (Fo) supplementation (Pusceddu et al 2015) or (Ex) (Batista-de-Oliveira et al 2012) depend on the animal age.…”

Section: R a F Tmentioning

confidence: 99%

“…The daily supplementation was performed by gavage. Rats received a single daily dose of fish oil (Fo, 85mg/kg/d and 1ml/250g/d) or vehicle solution (V, 1 ml/250g/d) as adapted from (Rachetti et al 2013, da Silva Pedroza et al 2015. The capsules of fish oil (Sundown®) containing polyunsaturated fatty acids [docoisahexaenoico (DHA; 85mg/1g) and ecoisapentaenoico (EPA; 128mg/1g)] were dissolved in Cremophor (Sigma ®) 0.009%, then in distilled water and were D r a f t administered via gavage.…”

Section: Supplementation With Fish Oilmentioning

confidence: 99%

Fish oil and treadmill exercise have age-dependent effects on episodic memory and oxidative state of the hippocampus

Macêdo

Melo

Costa

et al. 2017

Appl. Physiol. Nutr. Metab.

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There is a growing interest to better understand how lifestyle choices can improve memory functions. Treadmill exercise and long-chain n-3 polyunsaturated fatty acids found in fish oil are able to stimulate hippocampal antioxidant defenses and improve memory. The aim was to test whether fish oil and exercise can improve rat's performance on memory tasks and optimize hippocampal antioxidant state in an age-dependent manner. Therefore, young and adult rats were exercised and received fish oil during 4 weeks. The exercise was performed for 30 min/day, with the speed gradually increasing from the first to the last week. Afterwards, episodic memory was measured by the recognition of object identity and spatial location. Hippocampal oxidative state was investigated with the levels of malondialdehyde (MDA), carbonyls content, antioxidant enzymatic activity (superoxide dismutase (SOD), catalase (CAT)), and antioxidant nonenzymatic activity (reduced glutathione, sulfhydryl content). The adult rats treated with fish oil and exercise (FO&EX) were able to recognize object's shape and placement; however, FO&EX young rats had impaired spatial recognition (p < 0.05). The FO&EX young rats did not have reduced MDA or carbonyl content, though either fish oil or exercise reduced MDA (p < 0.05) and carbonyl levels (p < 0.01). Exercise increased SOD (p < 0.001) and CAT activities (p < 0.05), and fish oil enhanced SOD activity (p < 0.05) in young rats. At adulthood, exercise increased MDA levels (p < 0.05), and FO&EX reduced MDA (p < 0.001). Finally, exercise and fish oil improved nonenzymatic antioxidant defense (p < 0.05) only in adult rats. Results support age-dependent effects of fish oil and exercise on memory and oxidative state of the hippocampus during either neurodevelopment or adulthood.

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Can fish oil supplementation and physical training improve oxidative metabolism in aged rat hearts?

Cited by 9 publications

References 46 publications

Heart Histopathology and Mitochondrial Ultrastructure in Aged Rats Fed for 24 Months on Different Unsaturated Fats (Virgin Olive Oil, Sunflower Oil or Fish Oil) and Affected by Different Longevity

Heart Histopathology and Mitochondrial Ultrastructure in Aged Rats Fed for 24 Months on Different Unsaturated Fats (Virgin Olive Oil, Sunflower Oil or Fish Oil) and Affected by Different Longevity

Fish oil supplementation and physical exercise during the development period increase cardiac antioxidant capacity in Wistar rats

Fish oil and treadmill exercise have age-dependent effects on episodic memory and oxidative state of the hippocampus

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