Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Kashef, Nasim; Hamblin, Michael R.

doi:10.1016/j.drup.2017.07.003

Cited by 255 publications

(201 citation statements)

References 150 publications

(146 reference statements)

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“…It is well‐recognized that photodynamic inactivation attacks several biomolecules on bacterial cells, including proteins, nucleic acids and lipids . The damage induced by ROS in lipids diminishes the fluidity of the cell membrane causing its disruption, as a consequence of oxidative stress during aPDT . In fact, the electron micrographs obtained in the present study can reflect the morphological changes of bacterial membrane induced by photodynamic treatment and confirm the detrimental effects of photoinactivation mediated by ERY and RB.…”

Section: Resultssupporting

confidence: 91%

Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States

Silva

Santos

Trevisan

et al. 2019

Photochem & Photobiology

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This study evaluated the rose bengal‐ and erythrosine‐mediated photoinactivation against Salmonella Typhimurium and Staphylococcus aureus planktonic and sessile cells using green LED as a light source. The free‐living or 2‐day‐old biofilm cells were treated with different concentrations of the photosensitizing agents and subjected to irradiation. Only 5 min photosensitization with rose bengal at 25 nmol L−1 and 75 μmol L−1 completely eliminated S. aureus and S. Typhimurium planktonic cells, respectively. Erythrosine at 500 nmol L−1 and 5 min of light exposure also reduced S. aureus planktonic cells to undetectable levels. Eradication of S. aureus biofilms was achieved when 500 μmol L−1 of erythrosine or 250 μmol L−1 of rose bengal was combined with 30 min of irradiation. Scanning electron microscopy allowed the observation of morphological changes in planktonic cells and disruption of the biofilm architecture after photodynamic treatment. The overall data demonstrate that rose bengal and erythrosine activated by green LED may be a targeted strategy for controlling foodborne pathogens in both planktonic and sessile states.

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Section: Resultssupporting

confidence: 91%

Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States

Silva

Santos

Trevisan

et al. 2019

Photochem & Photobiology

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“…The unfortunate excessive use of antibiotics has led to a considerable and alarming increase in the rate of resistant isolates in many countries . Considering that the era of antibiotics will eventually come to an end, aPDT has been established as an effective antimicrobial strategy that does not rely on conventional antibiotic mechanisms . Despite a number of advantages, there have been very few studies in which PDT was considered as a treatment option for bladder infections, even though, in recent years, PDT of bladder cancer has found its way into widespread clinical use .…”

Section: Introductionmentioning

confidence: 97%

Gaining Access to Bacteria through (Reversible) Control of Lipophilicity

Galstyan

Putze

Dobrindt

2017

Chemistry A European J

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The development of antimicrobial photodynamic therapy (aPDT) is highly dependent on the development of suitable photosensitizers (PSs); ideally, affinity of a PS towards bacterial cells should be much higher than that towards mammalian cells. A cationic charge on a PS may lead to its selective binding to bacteria mediated through electrostatic interaction; however, the photodynamic outcome is highly dependent on the lipophilicity of the PS. Herein, we report the aPDT effect of silicon(IV) phthalocyanine derivatives bearing four positive charges and methyl, phenyl, or naphthyl substituents at the periphery of the macrocycle. We show that through modulation of lipophilicity, it is possible to find a therapeutic window in which bacteria, but not mammalian cells, are effectively killed. The photobiological activity of these PSs was significantly lower when they were deployed as host-guest complexes with cucurbit[7]uril (CB[7]). CB[7] blocks the hydrophobic part of the PS and reduces its lipophilicity, indicating that a hydrophobic interaction with the outer membrane of bacterial cells is essential for aPDT activity. The efficacies of the obtained PSs have been evaluated by using different uropathogenic E. coli isolates and human kidney epithelial carcinoma cells.

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“…Moreover, the phthalocyanine ZnPPc 4+ is also an efficient photosensitizer to kill planktonic bacterial cells . However, it is known that bacteria growing as biofilm are less susceptible to PDI compared with their equivalent planktonic form . Thereby, photosensitizer concentration and irradiation times were increased to gain similar photoinactivation.…”

Section: Discussionmentioning

confidence: 99%

Photodynamic inactivation to prevent and disrupt Staphylococcus aureus biofilm under different media conditions

Reynoso

Ferreyra

Durantini

et al. 2019

Photoderm Photoimm Photomed

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Objective The goal of this work was to investigate the photodynamic activity of 5,10,15,20‐tetrakis[4‐(3‐N,N‐dimethylaminopropoxy)phenyl]chlorin (TAPC) and zinc(II) 2,9,16,23‐tetrakis[4‐(N‐methylpyridyloxy)]phthalocyanine iodide (ZnPPc4+) as photosensitizers to inactivate Staphylococcus aureus biofilms and prevent their formations in different culture media. Methods We incubated S aureus biofilms in different culture media: tryptic soy (TS), nutrient (N), Müeller Hinton (MH) broth, TS with glucose 2 and 5% (w/v) with 5 μM ZnPPc4+ or TAPC and irradiated with visible light (350‐800 nm). Photodynamic inactivation (PDI) was determined by count of colony forming units (CFU) and crystal violet method. Furthermore, we studied PDI effect on biofilm development in TS broth. Finally, we examined the effects of PDI on the structure of S aureus biofilm. Results Greater inactivation was achieved, using TAPC or ZnPPc4+, when S aureus biofilm was grown in N or MH broths rather than in TS. Besides, glucose addition to the medium decreases the ability to develop biofilm and increase the photoinactivation capacity. Prevention of 3 log biofilm developments was obtained when S aureus cultures were treated with TAPC (10 μM) and 108 J/cm2 in TS broth and the number of CFU was counted after 24 hours. Moreover, microscopy studies demonstrated modifications in biofilm architecture. Conclusions These results indicate that TAPC and ZnPPc4+ may be promising photosensitizers for photodynamic inactivation of S aureus biofilms or to prevent their formation.

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Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Cited by 255 publications

References 150 publications

Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States

Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States

Gaining Access to Bacteria through (Reversible) Control of Lipophilicity

Photodynamic inactivation to prevent and disrupt Staphylococcus aureus biofilm under different media conditions

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