Can sibling species of the Drosophila willistoni subgroup be recognized through combined microscopy techniques?

Zanini, Rebeca; Deprá, Maríndia; Valente, Vera Lúcia da Silva

doi:10.1016/j.rbe.2015.09.006

Cited by 10 publications

(14 citation statements)

References 26 publications

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“…Although monomorphic non-melanic tergite color was the most common phenotype among saltans species (Supplementary Figure S3) (De Magalhaes, 1956), we observed male-limited pigmentation phenotypes for D. sturtevanti (Figure 2) and D. emarginata (Supplementary Figure S3). Within the willistoni species group, all species analyzed (Supplementary Figure S4) or reported in the literature (Zanini et al, 2015) exhibit a monomorphic nonmelanic tergite color.…”

Section: Sexually Dimorphic Pigmentation Is Widespread Within the Sopmentioning

confidence: 99%

Gene Regulatory Network Homoplasy Underlies Recurrent Sexually Dimorphic Fruit Fly Pigmentation

et al. 2020

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Traits that appear discontinuously along phylogenies may be explained by independent origins (homoplasy) or repeated loss (homology). While discriminating between these models is difficult, the dissection of gene regulatory networks (GRNs) which drive the development of such repeatedly occurring traits can offer a mechanistic window on this fundamental problem. The GRN responsible for the male-specific pattern of Drosophila (D.) melanogaster melanic tergite pigmentation has received considerable attention. In this system, a metabolic pathway of pigmentation enzyme genes is expressed in spatial and sex-specific (i.e., dimorphic) patterns. The dimorphic expression of several genes is regulated by the Bab transcription factors, which suppress pigmentation enzyme expression in females, by virtue of their high expression in this sex. Here, we analyzed the phylogenetic distribution of species with male-specific pigmentation and show that this dimorphism is phylogenetically widespread among fruit flies. The analysis of pigmentation enzyme gene expression in distantly related dimorphic and monomorphic species shows that dimorphism is driven by the similar deployment of a conserved metabolic pathway. However, sexually dimorphic Bab expression was found only in D. melanogaster and its close relatives. These results suggest that dimorphism evolved by parallel deployment of differentiation genes, but was derived through distinct architectures at the level of regulatory genes. This work demonstrates the interplay of constraint and flexibility within evolving GRNs, findings that may foretell the mechanisms of homoplasy more broadly.

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Section: Sexually Dimorphic Pigmentation Is Widespread Within the Sopmentioning

confidence: 99%

Gene Regulatory Network Homoplasy Underlies Recurrent Sexually Dimorphic Fruit Fly Pigmentation

et al. 2020

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“…In the preceding studies, some relationships were incongruent between data partitions and barely supported. Additionally, despite morphological studies [15][16][17][18][19][20][21], there is no phylogenetic reconstruction of the willistoni subgroup based on morphological characters or a strong time estimation of the cladistic events. Although past studies tried to differentiate D. paulistorum semispecies, some authors believe that there are no sufficient differences in the [19,22].…”

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confidence: 99%

Combining morphology and molecular data to improveDrosophila paulistorum(Diptera, Drosophilidae) taxonomic status

Zanini

Müller

Vieira

et al. 2018

Fly

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The willistoni species subgroup has been the subject of several studies since the latter half of the past century and is considered a Neotropical model for evolutionary studies, given the many levels of reproductive isolation and different evolutionary stages occurring within them. Here we present for the first time a phylogenetic reconstruction combining morphological characters and molecular data obtained from 8 gene fragments (COI, COII, Cytb, Adh, Ddc, Hb, kl-3 and per). Some relationships were incongruent when comparing morphological and molecular data. Also, morphological data presented some unresolved polytomies, which could reflect the very recent divergence of the subgroup. The total evidence phylogenetic reconstruction presented well-supported relationships and summarized the results of all analyses. The diversification of the willistoni subgroup began about 7.3 Ma with the split of D. insularis while D.paulistorum complex has a much more recent diversification history, which began about 2.1 Ma and apparently has not completed the speciation process, since the average time to sister species separation is one million years, and some entities of the D. paulistorum complex diverge between 0.3 and 1 Ma. Based on the obtained data, we propose the categorization of the former "semispecies" of D. paulistorum as a subspecies and describe the subspecies D. paulistorum amazonian, D. paulistorum andeanbrazilian, D. paulistorum centroamerican, D. paulistorum interior, D. paulistorum orinocan and D. paulistorum transitional.

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“…D. willistoni can be subdivided into three subspecies: D. w. willistoni , D. w. winge , and D. w. quechua ( Ayala and Tracey, 1973 ; Mardiros et al, 2016 ) , that have different geographic distributions. As shown in Figure 1 , D. willistoni has a predominantly neotropical distribution, from Mexico and south Florida to the southernmost part of South America and from the Pacific to the Atlantic oceans ( Spassky et al, 1971 ; Zanini et al, 2015 ). The strains used in the in-situ and in-silico analyses represent populations arranged along the geographic distribution of the different subspecies ( Figure 1 ): D. willistoni -Gd-H4-1 (Guadeloupe Island - willistoni subspecies), D. willistoni -WIP-4 (Bahia, Brazil - winge subspecies), and D. willistoni -SG12.00 (Montevideo, Uruguay - winge subspecies), used in in-situ and in-silico analyses; and D. willistoni-L17 (Uruguay - winge subspecies) and D. willistoni-00 (Santa Maria de Ostuna, Nicaragua - willistoni subspecies) used only in in-silico analyses.…”

Section: Discussionmentioning

confidence: 99%

“…, 2007 ). Strain Gd-H4-1 lacks the high degree of polymorphism and variability found in natural populations of this widely distributed tropical species (review by Zanini et al, 2015 ). Two additional strains of D. willistoni were recently sequenced by Kim et al (2021 ), who found considerable differences between these strains in the number of repetitive sequences such as transposons and microsatellite elements.…”

Section: Introductionmentioning

confidence: 99%

Interpopulation variation of transposable elements of the hAT superfamily in Drosophila willistoni (Diptera: Drosophilidae): in-situ approach

et al. 2022

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Transposable elements are abundant and dynamic part of the genome, influencing organisms in different ways through their presence or mobilization, or by acting directly on pre- and post-transcriptional regulatory regions. We compared and evaluated the presence, structure, and copy number of three hAT superfamily transposons ( hobo, BuT2 , and mar ) in five strains of Drosophila willistoni species . These D. willistoni strains are of different geographical origins, sampled across the north-south occurrence of this species. We used sequenced clones of the hAT elements in fluorescence in-situ hybridizations in the polytene chromosomes of three strains of D. willistoni . We also analyzed the structural characteristics and number of copies of these hAT elements in the 10 currently available sequenced genomes of the willistoni group. We found that hobo, BuT2, and mar were widely distributed in D. willistoni polytene chromosomes and sequenced genomes of the willistoni group, except for mar , which is restricted to the subgroup willistoni. Furthermore, the elements hobo, BuT2 , and mar have different evolutionary histories. The transposon differences among D. willistoni strains, such as variation in the number, structure, and chromosomal distribution of hAT transposons, could reflect the genomic and chromosomal plasticity of D. willistoni species in adapting to highly variable environments.

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Can sibling species of the Drosophila willistoni subgroup be recognized through combined microscopy techniques?

Cited by 10 publications

References 26 publications

Gene Regulatory Network Homoplasy Underlies Recurrent Sexually Dimorphic Fruit Fly Pigmentation

Gene Regulatory Network Homoplasy Underlies Recurrent Sexually Dimorphic Fruit Fly Pigmentation

Combining morphology and molecular data to improveDrosophila paulistorum(Diptera, Drosophilidae) taxonomic status

Interpopulation variation of transposable elements of the hAT superfamily in Drosophila willistoni (Diptera: Drosophilidae): in-situ approach

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