Cancer-Selective Treatment of Cancerous and Non-Cancerous Human Cervical Cell Models by a Non-Thermally Operated Electrosurgical Argon Plasma Device

Feil, Lukas; Koch, Andrè; Utz, Raphael; Ackermann, Michael; Barz, Jakob; Stope, Matthias B.; Krämer, B.; Wallwiener, D.; Brucker, Sara Y.; Weiß, Martin

doi:10.3390/cancers12041037

Cited by 24 publications

(22 citation statements)

References 35 publications

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“…With regard to cancer treatment, various strategies using different types of CAPs have recently shown promising effects in cellular tumor models of breast cancer 12 – 14 , cervical cancer 13 – 15 , liver cancer 16 , lung cancer 17 , skin cancer 18 – 22 , and other cancers 6 , 8 . Numerous therapeutic advantages of CAPs over treatments with conventional chemotherapeutic agents have been identified as a result of previous studies, the most important being probably the selective effect of cold plasma on normal and carcinoma cells 22 – 24 .…”

Section: Introductionmentioning

confidence: 99%

Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Pefani-Antimisiari

Athanasopoulos

Marazioti

et al. 2021

Sci Rep

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The aim of the present study was to investigate combined effects of cold atmospheric plasma (CAP) and the chemotherapeutic drug doxorubicin (DOX) on murine and human melanoma cells, and normal cells. In addition to free drug, the combination of CAP with a liposomal drug (DOX-LIP) was also studied for the first time. Thiazolyl blue tetrazolium bromide (MTT) and Trypan Blue exclusion assays were used to evaluate cell viability; the mechanism of cell death was evaluated by flow cytometry. Combined treatment effects on the clonogenic capability of melanoma cells, was also tested with soft agar colony formation assay. Furthermore the effect of CAP on the cellular uptake of DOX or DOX-LIP was examined. Results showed a strong synergistic effect of CAP and DOX or DOX-LIP on selectively decreasing cell viability of melanoma cells. CAP accelerated the apoptotic effect of DOX (or DOX-LIP) and dramatically reduced the aggressiveness of melanoma cells, as the combination treatment significantly decreased their anchorage independent growth. Moreover, CAP did not result in increased cellular uptake of DOX under the present experimental conditions. In conclusion, CAP facilitates DOX cytotoxic effects on melanoma cells, and affects their metastatic potential by reducing their clonogenicity, as shown for the first time.

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Section: Introductionmentioning

confidence: 99%

Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Pefani-Antimisiari

Athanasopoulos

Marazioti

et al. 2021

Sci Rep

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“…New therapy options are needed to treat the cancer and to reduce damage to normal tissues. For cancer-selective treatment, Feil et al [ 4 ] reported the effects of a non-thermally operated Martin Argon Plasma Beamer System (MABS) on cervical cancer cell lines and non-cancerous cervical tissue cells. Weiss et al [ 5 ] presented an electron-spin resonance spectroscopy on human tissue samples and treated with different cold atmospheric plasma (CAP) doses, allowing the distribution and the measurement of consequential radicals in the tissues.…”

Section: Introductionmentioning

confidence: 99%

Non-Thermal Biocompatible Plasma Jet Induction of Apoptosis in Brain Cancer Cells

Akter

Lim

Choi

et al. 2021

Cells

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Glioblastoma multiforme (GBM) is a highly malignant and rapidly advancing astrocytic brain tumor in adults. Current therapy possibilities are chemotherapy, surgical resection, and radiation. The complexity of drug release through the blood-brain barrier, tumor reaction to chemotherapy, and the inherent resistance of tumor cells present challenges. New therapies are needed for individual use or combination with conventional methods for more effective treatment and improved survival for patients. GBM is difficult to treat because it grows quickly, spreads finger-shaped tentacles, and creates an irregular margin of normal tissue surrounding the tumor. Non-thermal biocompatible plasma (NBP) has recently been shown to selectively target cancer cells with minimal effects on regular cells, acting by generating reactive oxygen species (ROS) and reactive nitrogen species (RNS). We applied a soft jet plasma device with a syringe shape to U87 MG cells and astrocytes. Our results show that NBP-J significantly inhibits cell proliferation and changes morphology, induces cell cycle arrest, inhibits the survival pathway, and induces apoptosis. Our results indicate that NBP-J may be an efficient and safe clinical device for brain cancer therapy.

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“…CAP applications in oncology have shown remarkable anticancer effects in vitro cell-lines, including, for example, melanoma [30], cutaneous squamous carcinoma [31], pancreatic [32], liver [33], gastric [34], colon [35], prostate or urinary bladder [36,37], breast [38][39][40][41][42][43][44], head and neck cancer [45], osteosarcoma [46,47], glioblastoma [48], lymphoma [49], acute myeloid leukaemia [50], multiple myeloma [51], human fibrosarcoma [52], or lung cancer [53], as well as in vivo solid tumour types in animal (mice) models, e.g., colon [54], breast [55,56], prostate cancer [57], cholangiocarcinoma [58], schwannoma [59], glioblastoma [60], or melanoma [61]. A limited number of studies have been published in oncogynaecology, however, mostly restricted to in vitro cell lines, e.g., cervical [12,[62][63][64][65][66][67][68][69], endometrial [70][71][72], or ovarian…”

Section: Introductionmentioning

confidence: 99%

Cold Atmospheric Pressure Plasma (CAP) as a New Tool for the Management of Vulva Cancer and Vulvar Premalignant Lesions in Gynaecological Oncology

Žúbor

Wang

Líšková

et al. 2020

IJMS

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Vulvar cancer (VC) is a specific form of malignancy accounting for 5–6% of all gynaecologic malignancies. Although VC occurs most commonly in women after 60 years of age, disease incidence has risen progressively in premenopausal women in recent decades. VC demonstrates particular features requiring well-adapted therapeutic approaches to avoid potential treatment-related complications. Significant improvements in disease-free survival and overall survival rates for patients diagnosed with post-stage I disease have been achieved by implementing a combination therapy consisting of radical surgical resection, systemic chemotherapy and/or radiotherapy. Achieving local control remains challenging. However, mostly due to specific anatomical conditions, the need for comprehensive surgical reconstruction and frequent post-operative healing complications. Novel therapeutic tools better adapted to VC particularities are essential for improving individual outcomes. To this end, cold atmospheric plasma (CAP) treatment is a promising option for VC, and is particularly appropriate for the local treatment of dysplastic lesions, early intraepithelial cancer, and invasive tumours. In addition, CAP also helps reduce inflammatory complications and improve wound healing. The application of CAP may realise either directly or indirectly utilising nanoparticle technologies. CAP has demonstrated remarkable treatment benefits for several malignant conditions, and has created new medical fields, such as “plasma medicine” and “plasma oncology”. This article highlights the benefits of CAP for the treatment of VC, VC pre-stages, and postsurgical wound complications. There has not yet been a published report of CAP on vulvar cancer cells, and so this review summarises the progress made in gynaecological oncology and in other cancers, and promotes an important, understudied area for future research. The paradigm shift from reactive to predictive, preventive and personalised medical approaches in overall VC management is also considered.

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Cancer-Selective Treatment of Cancerous and Non-Cancerous Human Cervical Cell Models by a Non-Thermally Operated Electrosurgical Argon Plasma Device

Cited by 24 publications

References 35 publications

Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells

Non-Thermal Biocompatible Plasma Jet Induction of Apoptosis in Brain Cancer Cells

Cold Atmospheric Pressure Plasma (CAP) as a New Tool for the Management of Vulva Cancer and Vulvar Premalignant Lesions in Gynaecological Oncology

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